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SirReal2

Catalog No. T6984   CAS 709002-46-0

SirReal2 is a potent and selective Sirt2 inhibitor with IC50 of 140 nM.

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SirReal2 Chemical Structure
SirReal2, CAS 709002-46-0
Pack Size Availability Price/USD Quantity
1 mg In stock $ 33.00
2 mg In stock $ 46.00
5 mg In stock $ 72.00
10 mg In stock $ 109.00
25 mg In stock $ 207.00
50 mg In stock $ 328.00
100 mg In stock $ 531.00
1 mL * 10 mM (in DMSO) In stock $ 83.00
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Purity: 99.75%
Purity: 98.96%
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Biological Description
Chemical Properties
Storage & Solubility Information
Description SirReal2 is a potent and selective Sirt2 inhibitor with IC50 of 140 nM.
Targets&IC50 SIRT2:140 nM
In vitro SirReal2 selectively inhibits Sirt2 via a Sirt2-specific amino acid network, and induces a tubulin hyperacetylation and a significant depletion of BubR1 in HeLa cells. [1]
In vivo In vivo, SirReal2 inhibits Sirt2 activity without affecting the activity of the other Class-I sirtuins Sirt1 and Sirt3. [1]
Kinase Assay Wee1 Mass Screening: Wee1 mass screening is performed using Amersham's p34cdc2 kinase SPA kit with some modifications. Briefly, 45–60 nM full-length Wee1 kinase is incubated with 25 μM compounds, 20 μM ATP, and 122–441 nM Cdc2/cyclin B in a final volume of 50 μl of enzyme dilution buffer [50 mM Tris (pH 8.0), 10 mM NaCl, 10 mM MgCl2, 1 mM DTT, and 0.1 mM Na3VO4]. After 30 min incubation at 30°C, 30 μl of [33P]ATP containing kinase buffer [67 mM Tris (pH 8.0), 40 mM NaCl, 13 mM MgCl2, 1 mM DTT, and 0.13 mM Na3VO4] containing 1 μM biotinylated peptide, and 0.25 μCi of [γ-33P]ATP is added to the reaction and incubated for another 30 min at 30°C. The reaction is stopped by adding 200 μl of stop buffer [50 μM ATP, 5 mM EDTA, 0.1% Triton X-100, and 1.25 mg/ml SPA beads in PBS]. After centrifugation at 2400 rpm for 15 min, the plate is counted with Wallac's Microbeta counter.
Cell Research HeLa cells were plated in petri dishes (5?cm), incubated overnight to a confluency of 30–40% and then treated with SirReal2 dissolved in RPMI1640 medium supplemented with fresh 20% (v/v) FCS, 1% (v/v) penicillin, 1% (v/v) streptomycin, 1% (v/v), L-glutamine, 1% (v/v) DMSO for 5?h at various concentrations. Cells were then washed with prewarmed PBS (2?ml), lysed in SDS–PAGE sample buffer (70?μl, 50?mM Tris/HCl, 0.5?mM EDTA, 1 × Complete Protease Inhibitors, 2% (v/v) IGEPAL, 2% (w/v) SDS, 10% (v/v) glycerol, 50?mM NCA, 3.3?μM trichostatin A, 50?mM DTT, 0.01% (w/v) bromophenol blue, pH 6.8) and sonicated (5?min). Cell samples were then separated using SDS–PAGE (12.5% (w/v) polyacrylamide), transferred to an activated nitrocellulose membrane (Bio-Rad), blocked with non-fat dry milk (Roth, 5% (w/v), TBS, 0.1% (v/v) Tween 20) and probed with an anti-acetyl-α-tubulin antibody (1:1,000) and an anti-GAPDH antibody (1:2,000–1:5,000) as a loading control.
Molecular Weight 420.55
Formula C22H20N4OS2
CAS No. 709002-46-0

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 42.1 mg/mL (100 mM)

TargetMolReferences and Literature

1. Rumpf T, et al. Nat Commun. 2015, 6, 6263.

Related compound libraries

This product is contained In the following compound libraries:
Highly Selective Inhibitor Library Microtubule-Targeted Compound Library Target-Focused Phenotypic Screening Library Anti-Diabetic Compound Library NO PAINS Compound Library DNA Damage & Repair Compound Library Anti-Cancer Compound Library Inhibitor Library Immunology/Inflammation Compound Library HIF-1 Signaling Pathway Compound Library

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Keywords

SirReal2 709002-46-0 Chromatin/Epigenetic DNA Damage/DNA Repair Sirtuin Inhibitor inhibit SirReal 2 SirReal-2 inhibitor

 

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