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SPD304

Catalog No. T12985L CAS 869998-49-2

SPD304 is a selective TNF-α inhibitor and blocks the interaction of TNF and its receptor. SPD304 has an IC50 of 22 µM for inhibiting in vitro TNF receptor 1 binding to TNF-α.

All products from TargetMol are for Research Use Only. Not for Human or Veterinary or Therapeutic Use.

SPD304, CAS 869998-49-2

Pack Size	Availability	Price/USD
500 μg	35 days	$ 75.00
1 mg	35 days	$ 140.00
5 mg	35 days	$ 563.00
10 mg	35 days	$ 980.00

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Biological Description

Chemical Properties

Storage & Solubility Information

Description	SPD304 is a selective TNF-α inhibitor and blocks the interaction of TNF and its receptor. SPD304 has an IC50 of 22 µM for inhibiting in vitro TNF receptor 1 binding to TNF-α.
Targets&IC50	TNF-α:22 μM
In vitro	SPD304 (2 μM) obviously rescues the survivability of aHSCs, reduces the production of lipid hydroxides, and increased intracellular GSH. The co-treatment of GA (75 μM) and SPD304 (2 μM), down-regulate TRADD almost 2-fold and p−RIP3 1.4−fold compared to GA alone and promotes caspase 8 activation [4].

Molecular Weight	547.61
Formula	C32H32F3N3O2
CAS No.	869998-49-2

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 24 mg/mL (43.83 mM), Sonication is recommended.

H2O: 18 mg/mL (32.87 mM), Sonication is recommended.

References and Literature

1. Molly M. He, et al. Small-Molecule Inhibition of TNF-α. Science 11 Nov 2005. 2. Alexiou P, et al. Rationally designed less toxic SPD-304 analogs and preliminary evaluation of their TNF inhibitory effects. Arch Pharm (Weinheim). 2014 Nov;347(11):798-805. 3. Mouhsine H, et al. Identification of an in vivo orally active dual-binding protein-protein interaction inhibitor targeting TNFα through combined in silico/in vitro/in vivo screening. Sci Rep. 2017 Jun 13;7(1):3424. 4. Gallic acid induces necroptosis via TNF-α signaling pathway in activated hepatic stellate cells. Chang YJ, et al. PLoS One. 2015 Mar 27;10(3):e0120713.

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL)，Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation：Take μL DMSO master liquid, next add μL PEG300， mix and clarify, next add μL Tween 80，mix and clarify, next add μL ddH₂O, mix and clarify.

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Be sure to add the solvent(s) in order. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

SPD304 869998-49-2 Apoptosis TNF TNF Receptor Tumor Necrosis Factor Receptor inhibit Inhibitor SPD 304 TNFR SPD-304 inhibitor

Products are chemical reagents for research use only and are not intended for human use. We do not sell to patients.