Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Reparixin (Repertaxin) is a potent inhibitor of both CXCL8 receptors CXCR1/2, it inhibits weakly CXCR2-mediated cell migration (IC50=100 nM), whereas it strongly blocks CXCR1-mediated chemotaxis (IC50=1 nM).
Pack Size | Availability | Price/USD | Quantity |
---|---|---|---|
1 mg | In stock | $ 48.00 | |
2 mg | In stock | $ 72.00 | |
5 mg | In stock | $ 122.00 | |
10 mg | In stock | $ 197.00 | |
25 mg | In stock | $ 413.00 | |
50 mg | In stock | $ 618.00 | |
100 mg | In stock | $ 879.00 | |
500 mg | In stock | $ 1,750.00 | |
1 mL * 10 mM (in DMSO) | In stock | $ 113.00 |
Description | Reparixin (Repertaxin) is a potent inhibitor of both CXCL8 receptors CXCR1/2, it inhibits weakly CXCR2-mediated cell migration (IC50=100 nM), whereas it strongly blocks CXCR1-mediated chemotaxis (IC50=1 nM). |
Targets&IC50 | CXCR1:1 nM, CXCR2:100 nM |
Cell Research | L1.2 Cell suspension (1.5-3×106 cells/mL) is incubated at 37°C for 15 min in the presence of vehicle or of Reparixin (1 nM-1 μM) and next seeded in triplicates in the upper compartment of the chemotactic chamber. Different agonists are seeded in the lower compartment of the chamber at the following concentrations: 1 nM CXCL8, 0.03 nM fMLP, 10 nM CXCL1, 2.5 nM CCL2, 30 nM C5a. The chemotactic chamber is incubated at 37°C in air with 5% CO2 for 45 min (human PMNs) or 2 h (monocytes). At the end of incubation, the filter is removed, fixed, and stained and five oil immersion fields at high magnification (100×) are counted for each migration well after sample coding. L1.2 migration is evaluated using 5 μM pore size Transwell filters. |
Animal Research | C57BL/6J mice (8-10 weeks old/20-25 g) are used. The subcutaneous administration of Reparixin (30 mg/kg) is performed 60 minutes before cerebral ischemia induction. The animals are divided into the following three experimental groups: Sham (i.e., the group in which the arteries are visualized, but there is no occlusion of the middle cerebral artery), Vehicle (i.e., the group pre-treated with the vehicle, phosphate buffer solution, 60 minutes before MCAo) and Reparixin (i.e., the group pre-treated with the drug 60 minutes before MCAo). To evaluate neurological signs secondary to MCAo, the animals are assessed with the SHIRPA battery 24 h after reperfusion. |
Synonyms | DF 1681Y, Repertaxin |
Molecular Weight | 283.39 |
Formula | C14H21NO3S |
CAS No. | 266359-83-5 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 500 mg/mL
You can also refer to dose conversion for different animals. More
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Reparixin 266359-83-5 Autophagy GPCR/G Protein Immunology/Inflammation CXCR DF 1681Y Inhibitor CXC chemokine receptors inhibit Repertaxin inhibitor