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PluriSIn 1

Catalog No. T1869   CAS 91396-88-2
Synonyms: NSC 14613, PluriSIn #1, PluriSln 1

PluriSIn 1 (NSC 14613) is an inhibitor of stearoyl-CoA desaturase (SCD1), the key enzyme in oleic acid biosynthesis, revealing a unique role for lipid metabolism in hPSCs.

All products from TargetMol are for Research Use Only. Not for Human or Veterinary or Therapeutic Use.
PluriSIn 1 Chemical Structure
PluriSIn 1, CAS 91396-88-2
Pack Size Availability Price/USD Quantity
5 mg In stock $ 31.00
10 mg In stock $ 52.00
25 mg In stock $ 84.00
50 mg In stock $ 122.00
100 mg In stock $ 178.00
200 mg In stock $ 253.00
500 mg In stock $ 425.00
1 mL * 10 mM (in DMSO) In stock $ 34.00
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Purity: 99.59%
Purity: 99.19%
Purity: 99%
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Biological Description
Chemical Properties
Storage & Solubility Information
Description PluriSIn 1 (NSC 14613) is an inhibitor of stearoyl-CoA desaturase (SCD1), the key enzyme in oleic acid biosynthesis, revealing a unique role for lipid metabolism in hPSCs.
Kinase Assay SCD1 activity assays: Cells are plated in 6-well plates at a density of 50k to 100k cells per well. 24 h later, 20 μM PluriSIn #1 or 0.2% DMSO-control are added to the cells. After 12 h of incubation at 37 ℃, 5% CO2, the old medium is removed, cells are washed with PBS, and new medium containing 2.3 μM of 0.75 UCi [1-14C] Stearic Acid is added. The cells are incubated for up to 4 h at 37 ℃, 5% CO2. After the incubation period, the medium is discarded and the cells are washed 3 times with 2 mL of PBS. 2 mL of the mixture n-hexane: isopropanol (3:2 v:v) are added, and the cells are incubated for 30 min at 37 ℃, 5% CO2. 2 mL Folch solution (chloroform: methanol,2:1,v:v) are subsequently added. The liquid is transferred to tubes for phase partition by adding 1 mL water. The lower organic phase is evaporated and used for lipid saponification and TLC separation of the free [1-14C] Stearic Acid (substrate) and [1-14C] Oleic Acid (formed product). Lipids extracted from the cells are applied to TLC plates previously immersed in 10% NO3 Ag and activated at 120℃x60 min. Unlabeled stearic and oleic acid are added to each application point as carriers and as internal standards for identification. The plates are run with a solvent mixture of Chloroform:MeOH:AcH:DDW (90:8:1:0.8). The free fatty acids are detected by U.V. after spraying the TLC with a 2',7',dichlorofluorescein solution. The spots corresponding to stearic and oleic acid are scraped and the radioactivity counted in a sc intillating counter. SCD1 desaturase activity is calculated from the percent conversion of substrate to product and the conversion to pmol/min/106 cells.
Cell Research Relative cell numbers are determined by fixating the cells with 0.5% glutardialdehyde and staining with methylene blue dissolved in 0.1 M boric acid (pH 8.5). Color extraction is performed using 0.1 M hydrochloric acid, and the staining (which is proportional to cell number) is quantitated by measuring absorbance at 650 nM.(Only for Reference)
Synonyms NSC 14613, PluriSIn #1, PluriSln 1
Molecular Weight 213.24
Formula C12H11N3O
CAS No. 91396-88-2

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 21.3 mg/mL (100 mM)

Ethanol: 21.3 mg/mL (100 mM)

TargetMolReferences and Literature

1. Ben-David U, et al. Cell Stem Cell, 2013, 12(2), 167-179.

TargetMolCitations

1. Zhang L, Yi Y, Wang T, et al.25-Hydroxycholesterol inhibits classical swine fever virus entry into porcine alveolar macrophages by depleting plasma membrane cholesterol.Veterinary Microbiology.2023: 109668.

Related compound libraries

This product is contained In the following compound libraries:
NO PAINS Compound Library Anti-Metabolism Disease Compound Library Apoptosis Compound Library Bioactive Compounds Library Max Anti-Obesity Compound Library Anti-Cancer Metabolism Compound Library Stem Cell Differentiation Compound Library Anti-Aging Compound Library Lipid Metabolism Compound Library Inhibitor Library

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Keywords

PluriSIn 1 91396-88-2 Apoptosis Metabolism Dehydrogenase Stearoyl-CoA Desaturase (SCD) PluriSIn-1 NSC14613 Inhibitor PluriSIn1 inhibit NSC 14613 PluriSIn #1 NSC-14613 PluriSln 1 inhibitor

 

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