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PIK-93

Catalog No. T2616 CAS 593960-11-3

Synonyms: PIK 93, PIK93

PIK-93 is the first potent, synthetic PI4K inhibitor with IC50 of 19 nM; inhibits PI3Kα with IC50 of 39 nM.

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PIK-93, CAS 593960-11-3

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Purity: 98%

Biological Description

Chemical Properties

Storage & Solubility Information

Description	PIK-93 is the first potent, synthetic PI4K inhibitor with IC50 of 19 nM; inhibits PI3Kα with IC50 of 39 nM.
Targets&IC50	PI3Kα:39 nM, PI4K:19 nM
Kinase Assay	Assay of PI3Ks: IC50 values are measured using a standard TLC assay for lipid kinase activity. Kinase reactions are performed by preparing areaction mixture containing kinase, PIK-93 (2% DMSO final concentration), buffer (25 mM HEPES, pH 7.4, 10 mM MgCl2), and freshly sonicated phosphatidylinositol (100 μg/ml). Reactions are initiated by the addition of ATP containing 10 μCi of γ-32P-ATP to a final concentration 10 or 100 μM, and allowed to proceed for 20 min at room temperature. For TLC analysis, reactions are then terminated by the addition of 105 μL 1N HCl followed by 160 μL CHCl3:MeOH (1:1). The biphasic mixture is vortexed, briefly centrifuged, and the organic phase transferred to a new tube using a gel loading pipette tip precoated with CHCl3. This extract is spotted on TLC plates and developed for 3 hours–4 hours in a 65:35 solution of n-propanol:1M acetic acid. The TLC plates are then dried, exposed to a phosphorimager screen, and quantitated. Kinase activity is typically measured at 10–12 concentrations of PIK-93 representing two-fold dilutions from the highest concentration of 100 μM.
Cell Research	For actin staining, dHL60 cells are preincubated in suspension with PIK-93 or vehicle for 40 min, centrifuged for 5 min at 2000 rpm at room temperature in a J6-B centrifuge, resuspended in mHBSS containing the respective agent at the same concentration, allowed to stick to fibronectin-covered coverslips, and subjected to stimulation with a uniform concentration of 100 nM f-Met-Leu-Phe (fMLP) for 3 min. Cellsare fixed in 3.7% PFA and stained with 10 units/mL rhodamine-phalloidin for 15 min.(Only for Reference)

Synonyms	PIK 93, PIK93
Molecular Weight	389.87
Formula	C14H16ClN3O4S2
CAS No.	593960-11-3

Storage

Powder: -20°C for 3 years

In solvent: -80°C for 2 years

Solubility Information

DMSO: 39 mg/mL (100 mM)

( < 1 mg/ml refers to the product slightly soluble or insoluble )

References and Literature

1. Knight ZA, Cell, 2006, 125(4), 733-747. 2. VanKeymeulen A, et al. Cell Biol. 2006, 174(3), 437-445. 3. Tóth B, et al. J BiolChem, 2006, 281(47), 36369-3637 4. Monet M, et al. J BiolChem, 2012, Epub ahead of print. 5. Arita M, et al. J Virol, 2011, 85(5), 2364-2372.

Related compound libraries

This product is contained In the following compound libraries：

Anti-Cancer Compound Library Autophagy Compound Library Anti-Lung Cancer Compound Library HIF-1 Signaling Pathway Compound Library Cancer Cell Differentiation Compound Library Glycometabolism Compound Library Anti-Cancer Metabolism Compound Library Anti-Obesity Compound Library Glutamine Metabolism Compound Library Anti-Pancreatic Cancer Compound Library

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL)，Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation：Take μL DMSO master liquid, next add μL PEG300， mix and clarify, next add μL Tween 80，mix and clarify, next add μL ddH₂O, mix and clarify.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

PIK-93 593960-11-3 DNA损伤和修复 PI3K/Akt/mTOR信号通路代谢微生物学 ATM/ATR Carbonic Anhydrase DNA-PK HSV mTOR PI3K PI4K Virus Protease Phosphatidylinositol 4 kinases PI4 kinases Phosphoinositide 3-kinase inhibit PIK 93 Inhibitor PIK93 inhibitor

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