Home Tools
Log in
Cart

NU1025

Catalog No. T6912   CAS 90417-38-2
Synonyms: NSC 696807

NU1025 (NSC-696807) is a potent PARP inhibitor with IC50 of 400 nM.

All products from TargetMol are for Research Use Only. Not for Human or Veterinary or Therapeutic Use.
NU1025 Chemical Structure
NU1025, CAS 90417-38-2
Pack Size Availability Price/USD Quantity
2 mg In stock $ 40.00
5 mg In stock $ 65.00
10 mg In stock $ 105.00
25 mg In stock $ 198.00
50 mg In stock $ 335.00
100 mg In stock $ 528.00
500 mg In stock $ 1,180.00
1 mL * 10 mM (in DMSO) In stock $ 65.00
Bulk Inquiry
Get quote
Select Batch  
Purity: 98.00%
Contact us for more batch information
Biological Description
Chemical Properties
Storage & Solubility Information
Description NU1025 (NSC-696807) is a potent PARP inhibitor with IC50 of 400 nM.
Targets&IC50 PARP:400 nM
In vitro NU1025 (0.2 mM) treatment attenuates Water2 induced cytotoxicity. NU1025 per se does not have any effect on cell viability. NU1025 pretreatment significantly increases cell viability (82.59 4.67%) in SIN-1 (0.8 mM) exposed cells.[2] NU1025 has no detectable effect on the proliferation of D54 and U251 cells. Treatment with NU1025 markedly inhibits the enhanced activation of PARP-1 induced by TPT and RT treatment.[3] No DNA strand breakage is detected following exposure to 200 μM NU1025 alone.[4]
In vivo Treatment with NU1025 (1 and 3 mg/kg) reduces the infarction to 25% and 45% versus vehicle treated rats, respectively. NU1025 (1 and 3 mg/kg) treatment significantly reduces edema volume. NU1025 also produces significant improvement in neurological deficits.[2]
Kinase Assay PARP activation assay: Cells are suspended in hypotonic buffer (9 mM HEPES, pH 7.8, 4.5% (v/v) dextran, 4.5 mM MgCl2 and 5 mM DTT) at 1.5 × 107/mL on ice for 30 min, then 9 vol of isotonic buffer (40 mM HEPES, pH 7.8, 130 mM KCl, 4% (v/v) dextran, 2 mM EGTA, 2.3 mM MgCl2, 225 mM sucrose and 2.5 mM DTT) is added. The reaction is started by adding 300 μL cells to 100 μL 300 μM NAD+ containing [32P]-NAD+, and terminated by the addition of 2 mL ice-cold 10% (w/v) TCA +10% (w/v) sodium pyrophosphate. After 30 min on ice the precipitated 32P-labelled ADP-ribose polymers are filtered, washed five times with 1% (v/v) TCA, 1% (v/v) sodium pyrophosphate, dried and counted.
Cell Research Cells are seeded in 96-well plates at a density of 2,500 cells/well and treated with the indicated doses of NU1025. Adherent cells are irradiated in medium with 250 kVp X-rays (dose rate 0.5 Gy/min). Untreated cells are used as a control. Following an up to 5 day incubation, cell proliferation is assessed by MTT assay.(Only for Reference)
Synonyms NSC 696807
Molecular Weight 176.17
Formula C9H8N2O2
CAS No. 90417-38-2

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 17.6 mg/mL (100 mM)

1eq. NaOH: 17.6 mg/mL (100 mM)

TargetMolReferences and Literature

1. McCabe N, et al. Cancer Biol Ther. 2005, 4(9), 934-936. 2. Kaundal RK, et al. Life Sci. 2006, 79(24), 2293-2302. 3. Sabbatino F, et al. Cytometry A. 2014, 85(11), 953-961. 4. Bowman KJ, et al. Br J Cancer. 2001, 84(1), 106-112.

Related compound libraries

This product is contained In the following compound libraries:
Anti-Cancer Active Compound Library Anti-Pancreatic Cancer Compound Library DNA Damage & Repair Compound Library NO PAINS Compound Library Anti-Ovarian Cancer Compound Library Epigenetics Compound Library Anti-Cancer Compound Library Inhibitor Library Anti-Prostate Cancer Compound Library Bioactive Compounds Library Max

Related Products

Related compounds with same targets
BYK204165 E7449 Fluzoparib DR2313 Cedrusin OUL35 Niraparib tosylate Talazoparib tosylate

TargetMolDose Conversion

You can also refer to dose conversion for different animals. More

TargetMol In vivo Formulation Calculator (Clear solution)

Step One: Enter information below
Dosage
mg/kg
Average weight of animals
g
Dosing volume per animal
ul
Number of animals
Step Two: Enter the in vivo formulation
% DMSO
%
% Tween 80
% ddH2O
Calculate Reset

TargetMolCalculator

Molarity Calculator
Dilution Calculator
Reconstitution Calculation
Molecular Weight Calculator
=
X
X

Molarity Calculator allows you to calculate the

  • Mass of a compound required to prepare a solution of known volume and concentration
  • Volume of solution required to dissolve a compound of known mass to a desired concentration
  • Concentration of a solution resulting from a known mass of compound in a specific volume
See Example

An example of a molarity calculation using the molarity calculator
What is the mass of compound required to make a 10 mM stock solution in 10 ml of water given that the molecular weight of the compound is 197.13 g/mol?
Enter 197.13 into the Molecular Weight (MW) box
Enter 10 into the Concentration box and select the correct unit (millimolar)
Enter 10 into the Volume box and select the correct unit (milliliter)
Press calculate
The answer of 19.713 mg appears in the Mass box

X
=
X

Calculator the dilution required to prepare a stock solution

Calculate the dilution required to prepare a stock solution
The dilution calculator is a useful tool which allows you to calculate how to dilute a stock solution of known concentration. Enter C1, C2 & V2 to calculate V1.

See Example

An example of a dilution calculation using the Tocris dilution calculator
What volume of a given 10 mM stock solution is required to make 20ml of a 50 μM solution?
Using the equation C1V1 = C2V2, where C1=10 mM, C2=50 μM, V2=20 ml and V1 is the unknown:
Enter 10 into the Concentration (start) box and select the correct unit (millimolar)
Enter 50 into the Concentration (final) box and select the correct unit (micromolar)
Enter 20 into the Volume (final) box and select the correct unit (milliliter)
Press calculate
The answer of 100 microliter (0.1 ml) appears in the Volume (start) box

=
/

Calculate the volume of solvent required to reconstitute your vial.

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial.
Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

g/mol

Enter the chemical formula of a compound to calculate its molar mass and elemental composition

Tip: Chemical formula is case sensitive: C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:
To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.
Definitions of molecular mass, molecular weight, molar mass and molar weight:
Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed n the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

bottom

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

NU1025 90417-38-2 Chromatin/Epigenetic DNA Damage/DNA Repair PARP repair anti-cancer cytotoxicity inhibit NU 1025 DNA NSC-696807 NU-1025 Inhibitor PAR Neuroprotective NSC696807 NAD poly ADP ribose polymerase NSC 696807 inhibitor

 

TargetMol