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NS-398

Catalog No. T3214 CAS 123653-11-2

Synonyms: N-(2-cyclohexyloxy-4-nitrophenyl)methane sulfonamide, NS398

NS-398 (N-(2-cyclohexyloxy-4-nitrophenyl)methane sulfonamide) is a COX-2 inhibitor wih anti-inflammatory activity.

All products from TargetMol are for Research Use Only. Not for Human or Veterinary or Therapeutic Use.

NS-398, CAS 123653-11-2

Pack Size	Availability	Price/USD
2 mg	In stock	$ 30.00
5 mg	In stock	$ 46.00
10 mg	In stock	$ 61.00
25 mg	In stock	$ 97.00
50 mg	In stock	$ 187.00
100 mg	In stock	$ 288.00
200 mg	In stock	$ 374.00
1 mL * 10 mM (in DMSO)	In stock	$ 51.00

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Purity: 99.72%

Purity: 99%

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Description	NS-398 (N-(2-cyclohexyloxy-4-nitrophenyl)methane sulfonamide) is a COX-2 inhibitor wih anti-inflammatory activity.
Targets&IC50	COX-2:3.8 μM
In vitro	NS-398 inhibits COX-2 enzyme activity in a concentration dependent manner, the IC50 being 3.8 μM, whereas NS-398 at 100 μM has no effect on COX-1 activity[1]. At 10 μM, NS-398 treatment results in increased production of COX-2 and the pro-inflammatory cytokine. NS-398 (10 μM) induces apoptosis in LNCaP cells, but not in the more aggressive, androgen-unresponsive C4-2b cells. The C4-2b cells are observed to continue to proliferate when treated with NS-398 and continues to retain malignant phenotype characteristics. NS-398 treatment results in C4-2b cell differentiation into an unusual neuroendocrinelike cell. These neuroendocrine-like cells produces both epithelial (cytokeratin 18 and prostate specific antigen) and neuronal (neuron-specific enolase and chromogranin A) proteins. Furthermore, this C4-2b cellular response to NS-398 is mediated by NF-kB transcription factor activation. NS-398 induces NF-kB and down-regulates Ikβ-α protein expression in LNCaP C4-2b cells[2].
In vivo	NS398 could inhibit Cox-2 expression induced by acoustic injury and could attenuate noise-induced hearing threshold shifts and cochlear hair cell loss. The inhibition of Cox-2 by NS398 could attenuate Noise-induced hearing loss(NIHL)and related hair cell damage.[3].
Cell Research	1×106 cells are plated in six well cluster plates with 2 ml of medium for 24 h. At time point 0, medium was removed, cells were carefully washed with phosphate buffered saline (PBS) and serum free(SF), phenol-red free medium containing 0.5 μg/ml BSA was added. Incubations are continued for an additional 72 h with or without increasing NS-398 concentrations. NS-398 stocks (5 mM) are dissolved in 0.1% dimethylsulfoxide (DMSO). Cells and culture medium are harvested at 24 h time intervals. Dead cells are removed by gentle washing with PBS and cell number determined by direct counting using trypan blue dye exclusion to identify viable cells. DMSO (0.1%) is added to control cultures.(Only for Reference)

Synonyms	N-(2-cyclohexyloxy-4-nitrophenyl)methane sulfonamide, NS398
Molecular Weight	314.36
Formula	C13H18N2O5S
CAS No.	123653-11-2

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 31.4 mg/mL (100 mM)

References and Literature

1. Futaki N, et al. Prostaglandins. 1994, 47(1):55-9. 2. Meyer-Siegler K, et al. Mol Med. 2001, 7(12):850-60. 3. Sun Y, et al. Sci Rep. 2016, 6:22573. 4. Wang Z, Liu P, Hu M, et al. A Research Article Submitted to Journal of Ethnopharmacology Naoxintong restores Ischemia Injury and inhibits Thrombosis via COX2-VEGF/NFκB Signaling[J]. Journal of Ethnopharmacology. 2021: 113809. 5. Wang H, Hu Y, He F, et al. All-trans retinoic acid and COX-2 cross-talk to regulate BMP9-induced osteogenic differentiation via Wnt/β-catenin in mesenchymal stem cells[J]. Biomedicine & Pharmacotherapy. 2019, 118: 109279.

Citations

1. Wang H, Hu Y, He F, et al. All-trans retinoic acid and COX-2 cross-talk to regulate BMP9-induced osteogenic differentiation via Wnt/β-catenin in mesenchymal stem cells. Biomedicine & Pharmacotherapy. 2019, 118: 109279 2. Wang Z, Liu P, Hu M, et al. A Research Article Submitted to Journal of Ethnopharmacology Naoxintong restores Ischemia Injury and inhibits Thrombosis via COX2-VEGF/NFκB Signaling. Journal of Ethnopharmacology. 2021: 113809.

Related compound libraries

This product is contained In the following compound libraries：

Inhibitor Library Anti-Cancer Active Compound Library Bioactive Compounds Library Max Anti-Prostate Cancer Compound Library ReFRAME Related Library Mitochondria-Targeted Compound Library Anti-Cardiovascular Disease Compound Library Target-Focused Phenotypic Screening Library Anti-Colorectal Cancer Compound Library Bioactive Lipid Compound Library

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Keywords

NS-398 123653-11-2 Immunology/Inflammation Neuroscience COX Inhibitor NS 398 inhibit N-(2-cyclohexyloxy-4-nitrophenyl)methane sulfonamide NS398 Cyclooxygenase inhibitor

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