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ML-7 hydrochloride

Catalog No. T3050 CAS 110448-33-4

Synonyms: ML-7 HCl

ML-7 hydrochloride (ML-7 HCl) is a cell-permeable, reversible, effective, ATP-competitive, and specific inhibitor of myosin light chain kinase (Ki: 300 nM); also inhibits smooth-muscle myosin light chain kinase, PKA, and PKC.

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ML-7 hydrochloride, CAS 110448-33-4

Pack Size	Availability	Price/USD
5 mg	In stock	$ 42.00
10 mg	In stock	$ 68.00
25 mg	In stock	$ 152.00
50 mg	In stock	$ 286.00
100 mg	In stock	$ 371.00
200 mg	In stock	$ 482.00
1 mL * 10 mM (in DMSO)	In stock	$ 46.00

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Purity: 99.61%

Purity: 99%

Biological Description

Chemical Properties

Storage & Solubility Information

Description	ML-7 hydrochloride (ML-7 HCl) is a cell-permeable, reversible, effective, ATP-competitive, and specific inhibitor of myosin light chain kinase (Ki: 300 nM); also inhibits smooth-muscle myosin light chain kinase, PKA, and PKC.
Targets&IC50	PKC:42 μM, PKA:21 μM, MLCK:0.3 μM(Ki)
In vitro	Inhibition of MLCK by ML-7 induces activation of caspase-3 in adherent MCF-10A and MCF-10A Ras-transformed cells[2]. Its treatment results in a dose-dependent decrease in MLC20 phosphorylation and a corresponding increase in cell death of SMC(smooth muscle cells). The inhibitory effect of ML-7 on MLCK is highly selective. The Ki of ML-7 for MLCK is 0.3 μM, while its Ki for protein kinase A is 21 μM and for protein kinase C is 42 μM. Overexpressing Bcl-2 can protect cells against apoptosis induced by ML-7[4].
In vivo	ML7 is able to improve Vascular endothelial dysfunction(VED) and atherosclerosis(AS) by regulating the expression of the tight junction (TJ) proteins zona occludens (ZO)-1 and occludin via mechanisms involving MLCK and MLC phosphorylation in high-fat diet-fed rabbits. ML7 decreases the expression of MLCK and MLC phosphorylation in the arterial wall of rabbits fed a high-fat diet and reduces lipid deposition lesions in AS rabbits[1].
Kinase Assay	IKK-2 Assay: Recombinant human IKK-2 (residues 1-756) is expressed in baculovirus as an N-terminal GST-tagged fusion protein, and its activity is assessed using a time-resolved fluorescence resonance energy transfer assay. In brief, IKK-2 (5 nM final) diluted in assay buffer (50 mM HEPES, 10 mM MgCl2, 1 mM CHAPS, pH 7.4, with 1 mM DTT and 0.01% w/v BSA) is added to wells containing various concentrations of compound or dimethyl sulfoxide (DMSO) vehicle (3% final). The reaction is initiated by the addition of GST-IκBα substrate (25 nM final)/ATP (1 μM final), in a total volume of 30 μL. The reaction is incubated for 30 min at room temperature, then terminated by the addition of 15 μL of 50 mM EDTA. Detection reagent (15 μL) in buffer (100 mM HEPES, pH 7.4, 150 mM NaCl, and 0.1% w/v BSA) containing antiphosphoserine- IκBα-32/36 monoclonal antibody 12C2, labeled with W-1024 europium chelate, and an allophycocyanin-labeled anti-GST antibody is added, and the reaction is further incubated for 60 min at room temperature. The degree of phosphorylation of GST- IκBαis measured as a ratio of specific 665-nm energy transfer signal to reference europium 620-nm signal, using a Packard Discovery plate reader.
Cell Research	Cells are treated with vehicle alone, Blebbistatin, ML-7, ML-9, latrunculin A or Y-27632 in media without serum at varying concentrations and time points before being fixed or prepared for FACS analysis. Untreated cells are also serum starved for the duration of the inhibitor treatment. Stock solutions of each agent are made in DMSO (50 μM Blebbistatin, 5 mM LA), 50% ethanol (10 mM ML-7), 70% ethanol (20 mM ML-9) or water (20 mM Y-27632) and are maintained at -20°C.(Only for Reference)

Synonyms	ML-7 HCl
Molecular Weight	452.74
Formula	C15H18ClIN2O2S
CAS No.	110448-33-4

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 22.6 mg/mL (50 mM)

References and Literature

1. Cheng X, et al. Mol Med Rep. 2015, 12(3):4109-16. 2. Connell LE, et al. J Cell Sci. 2006, 119(Pt 11):2269-81. 3. Saitoh M, et al. J Biol Chem. 1987, 262(16):7796-801. 4. Fazal F, et al. Mol Cell Biol. 2005, 25(14):6259-66. 5. Zhang H, Lin F, Huang J, et al. Anisotropic stiffness gradient-regulated mechanical guidance drives directional migration of cancer cells[J]. Acta Biomaterialia. 2020.

Citations

1. Zhang H, Lin F, Huang J, et al. Anisotropic stiffness gradient-regulated mechanical guidance drives directional migration of cancer cells. Acta Biomaterialia. 2020 2. Sun L, Sun L, Li X, et al. A Novel Tigecycline Adjuvant ML-7 Reverses the Susceptibility of Tigecycline-Resistant Klebsiella pneumoniae. Frontiers in cellular and infection microbiology. 2022: 1341.

Related compound libraries

This product is contained In the following compound libraries：

Inhibitor Library Tyrosine Kinase Inhibitor Library TGF-beta/Smad Compound Library Metabolism Compound Library Apoptosis Compound Library Bioactive Compound Library Bioactive Compounds Library Max Stem Cell Differentiation Compound Library Kinase Inhibitor Library Anti-Cancer Compound Library

Related Products

Related compounds with same targets

CID 2011756 Chymotrypsin 1-NM-PP1 CID-797718 R-IMPP BioE-1115 MLCK inhibitor peptide 18 acetate Cetraxate hydrochloride

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

ML-7 hydrochloride 110448-33-4 Cell Cycle/Checkpoint Chromatin/Epigenetic Cytoskeletal Signaling Metabolism Stem Cells Tyrosine Kinase/Adaptors Serine/threonin kinase YAP Myosin PKC PKA ML-7 Hydrochloride Inhibitor Yes-associated protein ML-7 HCl inhibit ML-7 ML7 hydrochloride ML7 ML7 Hydrochloride ML 7 ML 7 hydrochloride ML 7 Hydrochloride inhibitor

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