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Anti-Phospho-YAP1 (Ser127) Polyclonal Antibody is a Rabbit antibody targeting Phospho-YAP1 (Ser127). Anti-Phospho-YAP1 (Ser127) Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $221 | 7-10 days | |
100 μL | $373 | 7-10 days | |
200 μL | $527 | 7-10 days |
Description | Anti-Phospho-YAP1 (Ser127) Polyclonal Antibody is a Rabbit antibody targeting Phospho-YAP1 (Ser127). Anti-Phospho-YAP1 (Ser127) Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF. |
Alias | YAP1 (p-Ser127), YAP1 (p-S127), p-YAP1 (Ser127), p-YAP1 (S127), Phospho-YAP1 (S127) |
Ig Type | IgG |
Reactivity | Human (predicted:Mouse,Rat,Chicken,Dog,Horse,Rabbit) |
Verified Activity | 1. Tissue/cell: human bladder carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-Phospho-YAP1 (Ser127) Polyclonal Antibody, Unconjugated (TMAB-01530) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 2. Tissue/cell: human lung cancer; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-Phospho-YAP1 (Ser127) Polyclonal Antibody, Unconjugated (TMAB-01530) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. |
Application | IHC-P,IHC-Fr,IF |
Recommended Dose | IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
Antibody Type | Polyclonal |
Host Species | Rabbit |
Subcellular Localization | Cytoplasm. Nucleus. |
Construction | Hybridoma Polyclonal Antibody |
Purification | Protein A purified |
Appearance | Liquid |
Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
Research Background | This gene encodes the human ortholog of chicken YAP protein which binds to the SH3 domain of the Yes proto-oncogene product. This protein contains a WW domain that is found in various structural, regulatory and signaling molecules in yeast, nematode, and mammals, and may be involved in protein-protein interaction. [provided by RefSeq]. |
Immunogen | KLH conjugated Synthesised phosphopeptide: human YAP around the phosphorylation site of Ser127 |
Antigen Species | Human |
Gene ID | |
Uniprot ID | |
Biology Area | Signal transduction,Proto-oncogenes,Transcription Factors,Cytoplasmic,Src Family |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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