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Anti-Phospho-YAP1 (Ser127) Polyclonal Antibody

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Catalog No. TMAB-01530
Alias YAP1 (p-Ser127), YAP1 (p-S127), p-YAP1 (Ser127), p-YAP1 (S127), Phospho-YAP1 (S127)

Anti-Phospho-YAP1 (Ser127) Polyclonal Antibody is a Rabbit antibody targeting Phospho-YAP1 (Ser127). Anti-Phospho-YAP1 (Ser127) Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.

Anti-Phospho-YAP1 (Ser127) Polyclonal Antibody

Anti-Phospho-YAP1 (Ser127) Polyclonal Antibody

😃Good
Catalog No. TMAB-01530Alias YAP1 (p-Ser127), YAP1 (p-S127), p-YAP1 (Ser127), p-YAP1 (S127), Phospho-YAP1 (S127)
Anti-Phospho-YAP1 (Ser127) Polyclonal Antibody is a Rabbit antibody targeting Phospho-YAP1 (Ser127). Anti-Phospho-YAP1 (Ser127) Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.
Pack SizePriceAvailabilityQuantity
50 μL$2217-10 days
100 μL$3737-10 days
200 μL$5277-10 days
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Product Introduction

Bioactivity
Description
Anti-Phospho-YAP1 (Ser127) Polyclonal Antibody is a Rabbit antibody targeting Phospho-YAP1 (Ser127). Anti-Phospho-YAP1 (Ser127) Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.
AliasYAP1 (p-Ser127), YAP1 (p-S127), p-YAP1 (Ser127), p-YAP1 (S127), Phospho-YAP1 (S127)
Ig Type
IgG
Reactivity
Human (predicted:Mouse,Rat,Chicken,Dog,Horse,Rabbit)
Verified Activity
1. Tissue/cell: human bladder carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-Phospho-YAP1 (Ser127) Polyclonal Antibody, Unconjugated (TMAB-01530) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
2. Tissue/cell: human lung cancer; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-Phospho-YAP1 (Ser127) Polyclonal Antibody, Unconjugated (TMAB-01530) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
Application
IHC-P,IHC-Fr,IF
Recommended Dose
IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationCytoplasm. Nucleus.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundThis gene encodes the human ortholog of chicken YAP protein which binds to the SH3 domain of the Yes proto-oncogene product. This protein contains a WW domain that is found in various structural, regulatory and signaling molecules in yeast, nematode, and mammals, and may be involved in protein-protein interaction. [provided by RefSeq].
Antigen Details
Immunogen
KLH conjugated Synthesised phosphopeptide: human YAP around the phosphorylation site of Ser127
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
Signal transduction,Proto-oncogenes,Transcription Factors,Cytoplasmic,Src Family
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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