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Anti-Phospho-YAP1 (Ser127) Antibody (5B8)
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Catalog No. TMAB-01531
Anti-Phospho-YAP1 (Ser127) Antibody (5B8) is a Rabbit antibody targeting Phospho-YAP1 (Ser127). Anti-Phospho-YAP1 (Ser127) Antibody (5B8) can be used in WB,IHC-P,IHC-Fr,IF.
Anti-Phospho-YAP1 (Ser127) Antibody (5B8)
Anti-Phospho-YAP1 (Ser127) Antibody (5B8)
😃Good
Catalog No. TMAB-01531
Anti-Phospho-YAP1 (Ser127) Antibody (5B8) is a Rabbit antibody targeting Phospho-YAP1 (Ser127). Anti-Phospho-YAP1 (Ser127) Antibody (5B8) can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 25 μL | $154 | 7-10 days | |
| 50 μL | $273 | 7-10 days | |
| 100 μL | $487 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Antibody Type: Recombinant Rabbit Monoclonal Application: WB,IHC-P,IHC-Fr,IF Reactivity: Human,Mouse,Rat |
| Ig Type | IgG |
| Clone | 5B8 |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded Rat Stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Phospho-YAP1 (Ser127) Monoclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Phospho-YAP1 (Ser127) Monoclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Phospho-YAP1 (Ser127) Monoclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded Human Duodenum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Phospho-YAP1 (Ser127) Monoclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 5. Paraformaldehyde-fixed, paraffin embedded Human Fundus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Phospho-YAP1 (Ser127) Monoclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 6. Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Phospho-YAP1 (Ser127) Monoclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 7. NIH/3T3 (M) cells were treated with or without Calyculin A (100ng/ml) for 30 min, Hela (H) cells were treated with or without Okadaic A+ calyculin A (100 nM) for 60 min, 25 μg total protein per Lane of cell lysates probed with Phospho-YAP1 monoclonal antibody, unconjugated (TMAB-01531) at 1:5000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at RT for 60 min. |
| Application | WB,IHC-P,IHC-Fr,IF |
| Recommended Dose | WB: 1:500-5000; IHC-P: 1:50-200; IHC-Fr: 1:50-200; IF: 1:50-200 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cytoplasm. Nucleus. |
| Construction | Recombinant Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | This gene encodes the human ortholog of chicken YAP protein which binds to the SH3 domain of the Yes proto-oncogene product. This protein contains a WW domain that is found in various structural, regulatory and signaling molecules in yeast, nematode, and mammals, and may be involved in protein-protein interaction. [provided by RefSeq]. |
| Immunogen | A synthesized peptide: human YAP1 around the phosphorylation site of S127 |
| Antigen Species | Human |
| Gene ID | 10413 |
| Uniprot ID | |
| Biology Area | Signal transduction,Proto-oncogenes,Transcription Factors,Cytoplasmic,Src Family |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
Sci Citations
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