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Isosilybin

Catalog No. T3797 CAS 72581-71-6

Synonyms: Isosilybinin, Isosilibinin, Silymarin, Silybin B, Q-100795

Isosilybin (Isosilibinin) and Silybin might be suitable candidates to design potent PXR antagonists to prevent drug-drug interactions via CYP3A4 in cancer patients.

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Isosilybin, CAS 72581-71-6

Pack Size	Availability	Price/USD
1 mg	In stock	$ 30.00
5 mg	In stock	$ 68.00
10 mg	In stock	$ 105.00
25 mg	In stock	$ 228.00
50 mg	In stock	$ 425.00
100 mg	In stock	$ 628.00
1 mL * 10 mM (in DMSO)	In stock	$ 72.00

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Purity: 100%

Purity: 98%

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Description	Isosilybin (Isosilibinin) and Silybin might be suitable candidates to design potent PXR antagonists to prevent drug-drug interactions via CYP3A4 in cancer patients.
Targets&IC50	CYP3A4:74 μM.
In vitro	The reporter gene assay demonstrates that the milk thistle constituents silybin and isosilybin inhibit the PXR-mediated induction of CYP3A4, with isosilybin being a more potent inhibitor. Concentrations of 89, 133, and 200 μM of isosilybin decrease CYP3A4 induction by 64, 82, and 88%, respectively, and achieve an IC50 of 74 μM[1]. Additionally, the diastereoisomers isosilybin B and isosilybin A, derived from silymarin, exhibit anti-prostate cancer (PCA) activity through cell cycle arrest and apoptosis induction in human prostate cancer cells LNCaP and 22Rv1[2]. Isosilybin B enhances phosphorylation of Akt and Mdm2, leading to androgen receptor degradation, which is reversed by PI3K inhibitor pretreatment. This process facilitates the formation of a complex that promotes phosphorylation-dependent ubiquitination and subsequent degradation of the androgen receptor[3]. Isosilybin A significantly activates PPARγ at a 30 μM concentration, leading to a concentration-dependent transactivation of a PPARγ-dependent luciferase reporter. In silico docking studies indicate isosilybin A interacts with the receptor's ligand-binding domain through an additional hydrogen bond not found with inactive silymarin constituents[4].
Cell Research	LNCaP cells and 22Rv1 cells are plated and treated at 40–50% confluency with different doses of isosilybin B and isosilybin A (10–90 μM in medium) dissolved originally in Dimethyl sulfoxide (DMSO) for the desired time periods (24–48 h) in serum condition. An equal amount of DMSO (vehicle) is present in each treatment, including control; DMSO concentration did not exceed 0.1% (v/v) in any treatment. At the end of desired treatments, total cell number is determined by counting each sample in duplicate using a hemocytometer under an inverted microscope. Cell viability is determined using trypan blue exclusion method[2].
Source	Natural Products > Asteraceae > Silybum

Synonyms	Isosilybinin, Isosilibinin, Silymarin, Silybin B, Q-100795
Molecular Weight	482.44
Formula	C25H22O10
CAS No.	72581-71-6

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 88 mg/mL(182.4 mM)

Chloroform, Dichloromethane, Ethyl Acetate: Soluble

References and Literature

1. Mooiman KD, et al. Milk thistle's active components silybin and isosilybin: novel inhibitors of PXR-mediated CYP3A4 induction. Drug Metab Dispos. 2013 Aug;41(8):1494-504. 2. Deep G, et al. Isosilybin B and isosilybin A inhibit growth, induce G1 arrest and cause apoptosis in human prostate cancer LNCaP and 22Rv1 cells. Carcinogenesis. 2007 Jul;28(7):1533-42. 3. Deep G, et al. Isosilybin B causes androgen receptor degradation in human prostate carcinoma cells via PI3K-Akt-Mdm2-mediated pathway. Oncogene. 2008 Jun 26;27(28):31986-98. 4. Pferschy-Wenzig EM, et al. Identification of isosilybin a from milk thistle seeds as an agonist of peroxisome proliferator-activated receptor gamma. J Nat Prod. 2014 Apr 25;77(4):842-7.

Related compound libraries

This product is contained In the following compound libraries：

Inhibitor Library Selected Plant-Sourced Compound Library Ferroptosis Compound Library NO PAINS Compound Library Anti-Cancer Compound Library Anti-Aging Compound Library RO5 Drug-like Natural Product Library Terpene Natural Product Library Anti-Tumor Natural Product Library Traditional Chinese Medicine Monomer Library

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Keywords

Isosilybin 72581-71-6 Metabolism P450 Isosilybinin Cytochrome P450 Isosilibinin Inhibitor Q 100795 inhibit CYPs Silymarin Silybin B Q100795 Q-100795 inhibitor

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