Shopping Cart

Your shopping cart is currently empty

IWR-1

Name: IWR-1
Brand: TargetMol
SKU: T2651
Price: 50 USD
Availability: InStock
Rating: 5 (10 reviews)

(Synonyms: IWR-1-endo, endo-IWR 1) Copy Product Info

😃Good

Synonyms: IWR-1-endo, endo-IWR 1

Catalog No. T2651 Copy Product Info

Purity: 99.82%

😃Good

IWR-1 (IWR-1-endo) is a tankyrase inhibitor that targets the Wnt/β-catenin pathway, with an IC₅₀ of 180 nM. IWR-1 contributes to the activation of TCF/LEF and the expression of downstream targets of the Wnt/β-catenin pathway. IWR-1 promotes β-catenin phosphorylation by enhancing the stability of the Axin-mediated disassembly complex. IWR-1 can be used in research on diseases such as cancer and psoriasis.

Cas No. 1127442-82-3

Pack Size	Price	USA Stock	Global Stock
5 mg	$50	In Stock	In Stock
10 mg	$76	In Stock	In Stock
25 mg	$168	In Stock	In Stock
50 mg	$306	In Stock	In Stock
100 mg	$438	In Stock	In Stock
1 mL x 10 mM (in DMSO)	$50	In Stock	In Stock

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

Add to Cart

Add to Quotation

With extensive experience in compound synthesis, we can provide rapid custom synthesis services for this product according to your research needs.

For research use only—not for human use. No sales to individuals. Use as intended only.

Questions

Batch Information

Select Batch

Purity:99.82%

Color:White

Resource Download

COA HPLC HNMR

Product Introduction

Bioactivity

Description	IWR-1 (IWR-1-endo) is a tankyrase inhibitor that targets the Wnt/β-catenin pathway, with an IC₅₀ of 180 nM. IWR-1 contributes to the activation of TCF/LEF and the expression of downstream targets of the Wnt/β-catenin pathway. IWR-1 promotes β-catenin phosphorylation by enhancing the stability of the Axin-mediated disassembly complex. IWR-1 can be used in research on diseases such as cancer and psoriasis.
Targets & IC50	HT29 cells:24.4 μM, SW48 cells:2.5 μM (EC50), SW48 cells:0.25 μM, Wnt:0.2 μM (EC50), HEK293T cells:26 nM
In vitro	Methods: Human colorectal cancer cell lines HCT116 and HT29 were treated with IWR-1 at concentrations of 5, 10, 20, and 50 μM for 24 and 48 hours. Cell proliferation was assessed using the MTT/CCK-8 assay. Results: IWR-1 exhibited concentration- and time-dependent inhibition of cell proliferation. [1]
In vivo	Methods: Psoriasiform skin inflammation was induced in Balb/c mice by applying imiquimod cream to the dorsal skin daily for 5 consecutive days. On days 1, 3, and 5, mice received subcutaneous injections of IWR-1 (10 mg/kg) or IL-36γ (2 μg/mouse). After the fifth day of treatment, mice were euthanized, and dorsal skin tissue was harvested for subsequent analysis. Results: IL-36γ treatment significantly exacerbated IMQ-induced PASI scores, while IWR-1 treatment significantly suppressed IL-36γ-induced increases in PASI scores. [2]
Synonyms	IWR-1-endo, endo-IWR 1

Chemical Properties

Molecular Weight	409.44
Formula	C₂₅H₁₉N₃O₃
Cas No.	1127442-82-3
Smiles	O=C(Nc1cccc2cccnc12)c1ccc(cc1)N1C(=O)C2C3CC(C=C3)C2C1=O
Relative Density.	1.425 g/cm3 (Predicted)

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 85.8 mg/mL (209.55 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 4.1 mg/mL (10.01 mM), Suspension.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.4424 mL	12.2118 mL	24.4236 mL	122.1180 mL
5 mM	0.4885 mL	2.4424 mL	4.8847 mL	24.4236 mL
10 mM	0.2442 mL	1.2212 mL	2.4424 mL	12.2118 mL
20 mM	0.1221 mL	0.6106 mL	1.2212 mL	6.1059 mL
50 mM	0.0488 mL	0.2442 mL	0.4885 mL	2.4424 mL
100 mM	0.0244 mL	0.1221 mL	0.2442 mL	1.2212 mL

Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density.

Calculator

Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Molecular Weight Calculator

In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.