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Genistein

Catalog No. T1737 CAS 446-72-0

Synonyms: NPI 031L

Genistein (NPI 031L) is an isoflavonoid derived from soy products. It inhibits protein-tyrosine kinase and topoisomerase-II (DNA TOPOISOMERASES, TYPE II), with antineoplastic and antitumor activities.

All products from TargetMol are for Research Use Only. Not for Human or Veterinary or Therapeutic Use.

Genistein, CAS 446-72-0

Pack Size	Availability	Price/USD
50 mg	In stock	$ 38.00
100 mg	In stock	$ 54.00
500 mg	In stock	$ 96.00
1 mL * 10 mM (in DMSO)	In stock	$ 57.00

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Purity: 99.69%

Purity: 98.22%

Biological Description

Chemical Properties

Storage & Solubility Information

Description	Genistein (NPI 031L) is an isoflavonoid derived from soy products. It inhibits protein-tyrosine kinase and topoisomerase-II (DNA TOPOISOMERASES, TYPE II), with antineoplastic and antitumor activities.
Targets&IC50	EGFR:0.6 μM (IC50, Cell Assay)
In vitro	Genistein exhibits a preventive effect on breast and prostate cancer, as well as other endocrine-dependent tumors, in adult animals. In mice, when combined with radiation therapy for prostate tumors, Genistein can inhibit the growth of primary tumors and control the metastasis of tumor cells to the lymph nodes adjacent to the aorta.
In vivo	Genistein acts as an agonist for the GPR30 receptor and can bind to PPARγ with a dissociation constant (Ki) of 5.7 μM. In A431 epidermoid carcinoma cells, genistein inhibits tyrosine phosphorylation stimulated by EGF. Furthermore, in NIH-3T3 cells, genistein blocks mitogenic actions mediated by EGF, insulin, and thrombin.
Kinase Assay	Purified FXa is obtained after activation with Russell's viper venom followed by affinity chromatography. The resulting FXa is > 95% pure as judged by sodium dodecylsulfate polyacrylamide gel electrophoresis. The substrate affinity values for FXa, expressed as the Michaelis-Menten-Henri constant (Km), for human, rabbit, rat and dog FXa are determined using the chromogenic substrate S-2765, and are 36, 60, 240 and 70 μM, respectively. The substrate hydrolysis is monitored by measuring absorbance at 405 nm at 25°C for up to 30 min using a SpectraMax 384 Plus plate reader and SoftMax. FXa activity for each substrate and inhibitor concentration pair is determined in duplicate. The Ki values are calculated by non-linear least-squares fitting of the steady-state substrate hydrolysis rates to the equation for competitive inhibition (Equation 1) using GRAFIT, where v equals reactions velocity in OD min?1, Vmax equals maxiumum reaction velocity, S equals substrate concentration, and I equals inhibitor concentration.
Cell Research	Genistein is dissolved in DMSO and stored, and then diluted with appropriate medium before use[1]. The IC50 values for Genistein are determined by the MTT assay. Briefly, the MTT assay is a colorimetric assay that is based on the ability of living but not dead cells to reduce a tetrazolium-based compound to a blue formazan product. The formazan crystals are solubilized in DMSO, and the absorbance is measured at 540 nm. The absorbance at 540 nm is proportional to the number of viable cells. The lC50 values obtained with the MTT assay are compared with the lC50 values obtained by counting viable cells using trypan blue dye exclusion and by tritiated thymidine incorporation into DNA[1].
Source	Natural Products > Fabaceae > Trifolium

Synonyms	NPI 031L
Molecular Weight	270.24
Formula	C15H10O5
CAS No.	446-72-0

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 54 mg/mL (199.82 mM)

H2O: < 1 mg/mL (insoluble or slightly soluble)

Ethanol: 2 mg/mL (7.4 mM)

References and Literature

1. Akiyama T, et al. J Biol Chem, 1987, 262(12), 5592-5595. 2. Linassier C, et al. Biochem Pharmacol, 1990, 39(1), 187-193. 3. Dang ZC, et al. J Biol Chem, 2003, 278(2), 962-967. 4. Lamartiniere CA, et al. J Nutr, 2002, 132(3), 552S-558S. 5. Wang Y, et al. Radiat Res, 2006, 166(1 Pt 1), 73-80. 6. Jalili C, et al. Effect of Genistein on reproductive parameter and serum nitric oxide levels in morphine-treated mice. Int J Reprod Biomed (Yazd). 2016 Feb;14(2):95-102. 7. Ding S, et al. Environmentally Relevant Dose of Bisphenol A Does Not Affect Lipid Metabolism and Has No Synergetic or Antagonistic Effects on Genistein's Beneficial Roles on Lipid Metabolism. PLoS One. 2016 May 12;11(5):e0155352. 8. Zhou H, Ning Y, Zeng G, et al. Curcumin promotes cell cycle arrest and apoptosis of acute myeloid leukemia cells by inactivating AKT[J]. Oncology Reports. 2021, 45(4): 1-1.

Citations

1. Ferreira R S B, Farias F O, de Araujo E J S, et al.Deep eutectic solvents as an alternative for extraction of flavonoids from soybean (Glycine max (L) Merrill) and okara: An experimental and computational approach based on COSMO-SAC model.Food Research International.2023: 113266. 2. Qin H, Song Z, Zhao C, et al.miR-363-3p/PTEN is involved in the regulation of lipid metabolism by genistein in HepG2 cells via ERβ.Phytomedicine.2023: 154839. 3. Cui L, Xu Q, Zhang P, et al.Biochemical reprogramming of active-targeting and redox responsive nanoparticles for enhancing selective internalization.Materials Letters.2023: 134379. 4. Zhang X, Hong K, Sun Q, et al. Bioreducible, arginine-rich polydisulfide-based siRNA nanocomplexes with excellent tumor penetration for efficient gene silencing. Biomaterials Science. 2021, 9(15): 5275-5292. 5. Zhou H, Ning Y, Zeng G, et al. Curcumin promotes cell cycle arrest and apoptosis of acute myeloid leukemia cells by inactivating AKT. Oncology Reports. 2021, 45(4): 1-1.

Related compound libraries

This product is contained In the following compound libraries：

Kinase Inhibitor Library Inhibitor Library Anti-Cancer Clinical Compound Library Antiparasitic Natural Product Library Anti-Cancer Drug Library Tobacco Monomer Library FDA-Approved Kinase Inhibitor Library Drug Repurposing Compound Library Miao medicine Compound Library Anti-Cancer Approved Drug Library

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

Genistein 446-72-0 Angiogenesis Apoptosis Autophagy JAK/STAT signaling Metabolism Tyrosine Kinase/Adaptors EGFR Endogenous Metabolite NPI 031L HER1 ErbB-1 Inhibitor Epidermal growth factor receptor inhibit inhibitor

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