Metabolism PPAR Daidzein


Catalog No. T1238   CAS 486-66-8
Synonyms: Isoflavone

Daidzein is an isoflavone extract from soy, which is an inactive analog of the tyrosine kinase inhibitor genistein. It has antioxidant and phytoestrogenic properties.

Daidzein, CAS 486-66-8
Pack Size Availability Price/USD Quantity
5 mg In stock 16.00
10 mg In stock 20.00
25 mg In stock 30.00
50 mg In stock 38.00
100 mg In stock 43.00
200 mg In stock 50.00
500 mg In stock 60.00
1 g In stock 75.00
1 mL * 10 mM (in DMSO) In stock 54.00
Bulk Inquiry
Select Batch  
Biological Description
Chemical Properties
Storage & Solubility Information
Description Daidzein is an isoflavone extract from soy, which is an inactive analog of the tyrosine kinase inhibitor genistein. It has antioxidant and phytoestrogenic properties.
Targets&IC50 PPAR,  
In vivo Daidzein results in reduced body weight, a fact that may be explained by reduced feed consumption in female rats. Daidzein results in slight, but not significant, decreases in ovarian and uterine weights, and mammary gland size in female rats. [5]
Cell Research
Daidzein is dissolved in DMSO and then diluted with appropriate media[1]. HEK293T cells are plated on 24-well plates at a cell density of approximately 2.5×104 cells/well and are grown to 70-80% confluence. Cells are then transiently transfected with a PPAR-α or PPAR-γ expression plasmid, and a plasmid containing the luciferase gene under the control of three tandem PPAR response elements (PPRE × 3 TK-luciferase) using an X-treme GENE HP DNA Transfection Reagent. Renilla luciferase control vectors are co-transfected to control for transfection efficiency. After transfection, cells are cultured for another 24 h in medium containing DMSO or various concentrations (6.25, 12.5, 25 μM) of Daidzein. Cells are lysed, and luciferase activity is measured and expressed as fold induction, that is normalized to the activity of the renilla luciferase control plasmid[1].
Animal Research
Animal Model: C57 (C57 bl/6) and Apoe KO (C57 background) mice
Synonyms Isoflavone
Purity 98.84%
Molecular Weight 254.24
Formula C15H10O4
CAS No. 486-66-8


0-4℃ for short term (days to weeks), or -20℃ for long term (months).

Solubility Information

DMSO: 25.4 mg/mL (100 mM)

( < 1 mg/ml refers to the product slightly soluble or insoluble )


References and Literature
1. Hämäläinen M, et al. Mediators Inflamm. 2007;22007:45673. 2. Sugimoto E, et al. Biochem Pharmacol,2000, 59(5), 471-475. 3. Jia TL, et al. Biochem Pharmacol,2003, 65(5), 709-715. 4. Gao YH, et al. Mol Cell Biochem, 1999, 194(1-2), 93-97. 5. Lamartiniere CA, et al. Toxicol Sci,2002, 65(2), 228-238. 6. Kim E, et al. Daidzein Augments Cholesterol Homeostasis via ApoE to Promote Functional Recovery in Chronic Stroke. J Neurosci. 2015 Nov 11;35(45):15113-26. 7. Sakamoto Y1, et al. The Dietary Isoflavone Daidzein Reduces Expression of Pro-Inflammatory Genes through PPARα/γ and JNK Pathways in Adipocyte and Macrophage Co-Cultures. PLoS One. 2016 Feb 22;11(2):e0149676.

Related compound libraries

This product is contained In the following compound libraries:
Bioactive Compound Library Inhibitor Library Natural Compound Library for HTS Anti-cancer Compound Library Clinical Compound Library Anti-diabetic Compound Library Stem cell Differentiation Compound Library Neuronal Signaling Compound Library Endocrinology-Hormones Library Selected Plant-sourced Compound Library DNA Damage & Repair Compound Library GPCR Compound Library Anti-Metabolism Disease Compound Library Anti-Cardiovascular Disease Compound Library NF-κB Signaling Compound Library CNS-Penetrant Compound Library

Related Products

Related compounds with same targets
INT-131 12-O-Methylcarnosic acid Oleoylethanolamide GW1929 GW 6471 3-Guanidinopropionic Acid Growth hormone releasing peptide Ailanthoidol

Dose Conversion

Safe and effective drug dosing is necessary, regardless of its purpose of administration. Learn More

In vivo Formulation Calculator (Clear solution)

Step One: Enter information below
Average weight of animals
Dosing volume per animal
Number of animals
Step Two: Enter the in vivo formulation
% Tween 80
% ddH2O
Calculate Reset


Molarity Calculator
Dilution Calculator
Reconstitution Calculation
Molecular Weight Calculator

Molarity Calculator allows you to calculate the

  • mass of a compound required to prepare a solution of known volume and concentration
  • volume of solution required to dissolve a compound of known mass to a desired concentration
  • concentration of a solution resulting from a known mass of compound in a specific volume
See Example

An example of a molarity calculation using the molarity calculator
What is the mass of compound required to make a 10 mM stock solution in 10 ml of water given that the molecular weight of the compound is 197.13 g/mol?
Enter 197.13 into the Molecular Weight (MW) box
Enter 10 into the Concentration box and select the correct unit (millimolar)
Enter 10 into the Volume box and select the correct unit (milliliter)
Press calculate
The answer of 19.713 mg appears in the Mass box


Calculator the dilution required to prepare a stock solution

Calculate the dilution required to prepare a stock solution
The dilution calculator is a useful tool which allows you to calculate how to dilute a stock solution of known concentration. Enter C1, C2 & V2 to calculate V1.

See Example

An example of a dilution calculation using the Tocris dilution calculator
What volume of a given 10 mM stock solution is required to make 20ml of a 50 μM solution?
Using the equation C1V1 = C2V2, where C1=10 mM, C2=50 μM, V2=20 ml and V1 is the unknown:
Enter 10 into the Concentration (start) box and select the correct unit (millimolar)
Enter 50 into the Concentration (final) box and select the correct unit (micromolar)
Enter 20 into the Volume (final) box and select the correct unit (milliliter)
Press calculate
The answer of 100 microliter (0.1 ml) appears in the Volume (start) box


Calculate the volume of solvent required to reconstitute your vial

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial.
Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Enter the chemical formula of a compound to calculate its molar mass and elemental composition

Tip: Chemical formula is case sensitive: C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:
To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.
Definitions of molecular mass, molecular weight, molar mass and molar weight:
Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.


Tech Support

Answers to questions you may have can be found in the Inhibitor Handling Instructions. Topics include how to prepare stock solutions, how to store Products, and issues that need special attention for cell-based assays and animal experiments.