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Metabolism PPAR Daidzein

Daidzein

Catalog No. T1238   CAS 486-66-8
Synonyms: Isoflavone

Daidzein is an isoflavone extract from soy, which is an inactive analog of the tyrosine kinase inhibitor genistein. It has antioxidant and phytoestrogenic properties.

Daidzein, CAS 486-66-8
Pack Size Availability Price/USD Quantity
5 mg In stock 16.00
10 mg In stock 20.00
25 mg In stock 30.00
50 mg In stock 38.00
100 mg In stock 43.00
200 mg In stock 50.00
500 mg In stock 60.00
1 g In stock 75.00
1 mL * 10 mM (in DMSO) In stock 54.00
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Biological Description
Chemical Properties
Storage & Solubility Information
Description Daidzein is an isoflavone extract from soy, which is an inactive analog of the tyrosine kinase inhibitor genistein. It has antioxidant and phytoestrogenic properties.
Targets&IC50 PPAR,  
In vivo Daidzein results in reduced body weight, a fact that may be explained by reduced feed consumption in female rats. Daidzein results in slight, but not significant, decreases in ovarian and uterine weights, and mammary gland size in female rats. [5]
Cell Research
Daidzein is dissolved in DMSO and then diluted with appropriate media[1]. HEK293T cells are plated on 24-well plates at a cell density of approximately 2.5×104 cells/well and are grown to 70-80% confluence. Cells are then transiently transfected with a PPAR-α or PPAR-γ expression plasmid, and a plasmid containing the luciferase gene under the control of three tandem PPAR response elements (PPRE × 3 TK-luciferase) using an X-treme GENE HP DNA Transfection Reagent. Renilla luciferase control vectors are co-transfected to control for transfection efficiency. After transfection, cells are cultured for another 24 h in medium containing DMSO or various concentrations (6.25, 12.5, 25 μM) of Daidzein. Cells are lysed, and luciferase activity is measured and expressed as fold induction, that is normalized to the activity of the renilla luciferase control plasmid[1].
Animal Research
Animal Model: C57 (C57 bl/6) and Apoe KO (C57 background) mice
Synonyms Isoflavone
Purity 98.84%
Molecular Weight 254.24
Formula C15H10O4
CAS No. 486-66-8

Storage

0-4℃ for short term (days to weeks), or -20℃ for long term (months).

Solubility Information

DMSO: 25.4 mg/mL (100 mM)

( < 1 mg/ml refers to the product slightly soluble or insoluble )

Citations

References and Literature
1. Hämäläinen M, et al. Mediators Inflamm. 2007;22007:45673. 2. Sugimoto E, et al. Biochem Pharmacol,2000, 59(5), 471-475. 3. Jia TL, et al. Biochem Pharmacol,2003, 65(5), 709-715. 4. Gao YH, et al. Mol Cell Biochem, 1999, 194(1-2), 93-97. 5. Lamartiniere CA, et al. Toxicol Sci,2002, 65(2), 228-238. 6. Kim E, et al. Daidzein Augments Cholesterol Homeostasis via ApoE to Promote Functional Recovery in Chronic Stroke. J Neurosci. 2015 Nov 11;35(45):15113-26. 7. Sakamoto Y1, et al. The Dietary Isoflavone Daidzein Reduces Expression of Pro-Inflammatory Genes through PPARα/γ and JNK Pathways in Adipocyte and Macrophage Co-Cultures. PLoS One. 2016 Feb 22;11(2):e0149676.

Related compound libraries

This product is contained In the following compound libraries:
Bioactive Compound Library Inhibitor Library Natural Compound Library for HTS Anti-cancer Compound Library Clinical Compound Library Anti-diabetic Compound Library Stem cell Differentiation Compound Library Neuronal Signaling Compound Library Endocrinology-Hormones Library Selected Plant-sourced Compound Library DNA Damage & Repair Compound Library GPCR Compound Library Anti-Metabolism Disease Compound Library Anti-Cardiovascular Disease Compound Library NF-κB Signaling Compound Library CNS-Penetrant Compound Library

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