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Cisapride

Catalog No. T1291 CAS 81098-60-4

Synonyms: Pridesia, Kaudalit, Prepulsid, R 51619, 西沙比利, (±)-Cisaprid, Kinestase, 西沙必利

Cisapride is a serotonin type 4 (5-HT4) receptor agonists are potent prokinetic agents that act on serotonin receptors in the intestine and promote intestinal peristalsis, increase gastric emptying and decrease esophageal reflux.

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Cisapride, CAS 81098-60-4

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Purity: 98%

Biological Description

Chemical Properties

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Description	Cisapride is a serotonin type 4 (5-HT4) receptor agonists are potent prokinetic agents that act on serotonin receptors in the intestine and promote intestinal peristalsis, increase gastric emptying and decrease esophageal reflux.
Targets&IC50	5-HT4 Receptor:140 nM(EC50), hERG channel:9.4 nM
In vitro	Cisapride inhibits vascular Kv current independently of serotonin 5-HT4-receptor activation[2]. As HERG channel blocker, cisapride, can inhibit the growth of gastric cancer cells by altering distribution of cell cycle and inducing apoptosis so as to be of potential value in the treatment of gastric cancer. Cisapride could inhibit the growth and clonogenicity of human gastric cancer lines by specific blockage of HERG channel in a time- and dose-dependent manner while has little effects on GES cells[3].
Cell Research	Cells in the logarithmic growth phase are harvested and seeded in 96-well plates. The cell number is diluted to 5000/well in 200 microliters of RPMI1640 medium. In vitro experiments are carried out on the gastric cancer cell lines and GES cell line, using an MTT proliferation assay. MTT stains live cells, and the optical density absorbance at 490 nM wavelength correlates with the cell number. Cisapride is added to the cells at concentrations of 0, 12.5, 25, 50, 100 or 200 nM and equivalent volume of ethanol is added as control in order to eliminate the effects of solvent. Twenty microliters of MTT is added to each well of the cell culture 4 h before termination of the culture at 37?C. One hundred and fifty microliters of dimethyl sulfoxide is added to each well at the end of the culture and the plate is agitated for 10 minutes. The absorbency at 490 nm is read by a BIOHIT BP800 plate reader. Growth curve is drawn according to MTT colorimetry. The inhibition rate is calculated.(Only for Reference)

Synonyms	Pridesia, Kaudalit, Prepulsid, R 51619, 西沙比利, (±)-Cisaprid, Kinestase, 西沙必利
Molecular Weight	465.95
Formula	C23H29ClFN3O4
CAS No.	81098-60-4

Storage

Powder: -20°C for 3 years

In solvent: -80°C for 2 years

Solubility Information

DMSO: 46.6 mg/mL(100 mM)

( < 1 mg/ml refers to the product slightly soluble or insoluble )

References and Literature

1. Park JS, et al. Eur J Med Chem. 2016, 109:75-88. 2. Kim HW, et al.Biochem Biophys Res Commun. 2016, 478(3):1423-8. 3. Shao XD, et al. Cancer Biol Ther. 2005, 4(3):295-301.

Related compound libraries

This product is contained In the following compound libraries：

Anti-Lung Cancer Compound Library GPCR Compound Library Serotonin Receptor-Targeted Compound Library Neurotransmitter Receptor Compound Library Anti-Pancreatic Cancer Compound Library Ion Channel Inhibitor Library Immunology/Inflammation Compound Library Anti-Alzheimer's Disease Compound Library Fluorochemical Library Drug Repurposing Compound Library

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Keywords

Cisapride 81098-60-4 G蛋白偶联受体 JAK/STAT信号通路蛋白酪氨酸激酶离子通道神经科学血管生成 5-HT Receptor HER Potassium Channel 5-hydroxytryptamine Receptor prokinetic agent 5-HT4 agonist hERG Inhibitor R-51619 patch-clamp R 51619 (±)-Cisaprid Serotonin Receptor Kinestase inhibit Prepulsid R51619 gastrointestinal Pridesia Kaudalit inhibitor

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