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Capsaicin

Catalog No. T1062   CAS 404-86-4
Synonyms: Qutenza, 8-Methyl-N-vanillyl-trans-6-nonenamide, Vanilloid, (E)-Capsaicin, Zostrix

Capsaicin ((E)-Capsaicin) is a natural product extracted from Capsicum annuum, and is a TRPV1 agonist (EC50=0.29 μM). Capsaicin has antitumor, anti-inflammatory, antioxidant and neuroprotective activities.

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Capsaicin Chemical Structure
Capsaicin, CAS 404-86-4
Pack Size Availability Price/USD Quantity
50 mg In stock $ 50.00
100 mg In stock $ 75.00
1 mL * 10 mM (in DMSO) In stock $ 54.00
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Purity: 99.41%
Purity: 99.27%
Purity: 98.28%
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Biological Description
Chemical Properties
Storage & Solubility Information
Description Capsaicin ((E)-Capsaicin) is a natural product extracted from Capsicum annuum, and is a TRPV1 agonist (EC50=0.29 μM). Capsaicin has antitumor, anti-inflammatory, antioxidant and neuroprotective activities.
Targets&IC50 TRPV1 (HEK293 cells):0.29 μM(EC50)
In vitro METHODS: Human pharyngeal squamous carcinoma cells FaDu were treated with Capsaicin (50-300 µM) for 24-72 h. Cell viability was assayed using MTT Assay.
RESULTS: As the dose of Capsaicin increased, a decrease in enhanced cell growth was shown. Percentage of viable cells decreased with increase in incubation time. The IC50 value was about 150 µM. [1]
METHODS: Human oral epidermoid carcinoma cells KB were treated with Capsaicin (150-250 µM) for 24-48 h. Apoptosis was detected using Hoechst staining.
RESULTS: Capsaicin induced apoptosis in KB cells. [2]
In vivo METHODS: To investigate the effects on thermoregulation and locomotor activity, Capsaicin (10-20 mg/kg, saline+3% ethanol+10% Tween 80) was administered by single gavage to C57BL/6J mice with WT and TRPV1 KO.
RESULTS: Oral administration of capsaicin resulted in a long-term increase in TRPV1-dependent acute hypothermia and TRPV1-independent locomotor activity, in addition to activation of brain circuits controlling thermoregulation and metabolism. [3]
METHODS: To assay neuroprotective activity, Capsaicin (5-20 mg/kg) was administered orally to mice given scopolamine once daily for seven days.
RESULTS: Capsaicin exerted empirical neuroprotective effects through restoration of mitochondrial function, antioxidant effects and modulation of pro-inflammatory cytokines. A 10 mg/kg dose of Capsaicin for seven consecutive days was the most effective dose. [4]
Cell Research Capsaicin is dissolved in DMSO and stored, and then diluted with appropriate medium before use[3]. FaDu cells are plated at a density of 1×105 cells/well on 24-well plate. After overnight growth, the cells are treated with various concentrations of Capsaicin (0 μM, 50 μM, 100 μM, 150 μM, 200 μM, 250 μM, 300 μM, and 350 μM) for 24, 48 and 72 hours, with medium replacement every 24 hours. At the end of treatment, 30 μL of the tetrazolium compound MTT, and 270 μL of fresh medium are added. After further incubation for 4 hours at 37°C, 200 μL of 0.1 N HCl in 10% SDS is added into each well to dissolve the tetrazolium crystals. Finally, the absorbance at a wavelength of 540 nm is recorded using an ELISA plate reader[3].
Source
Synonyms Qutenza, 8-Methyl-N-vanillyl-trans-6-nonenamide, Vanilloid, (E)-Capsaicin, Zostrix
Molecular Weight 305.41
Formula C18H27NO3
CAS No. 404-86-4

Storage

keep away from direct sunlight

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 50 mg/mL (163.71 mM)

TargetMolReferences and Literature

1. Le TD, et al. Capsaicin-induced apoptosis of FaDu human pharyngeal squamous carcinoma cells. Yonsei Med J. 2012 Jul 1;53(4):834-4doi: 10.3349/ymj.2012.53.4.834. Erratum in: Yonsei Med J. 2012 Nov 1;53(6):1228. 2. Lin CH, et al. Capsaicin induces cell cycle arrest and apoptosis in human KB cancer cells. BMC Complement Altern Med. 2013 Feb 25;13:46. 3. Inagaki H, et al. Oral gavage of capsaicin causes TRPV1-dependent acute hypothermia and TRPV1-independent long-lasting increase of locomotor activity in the mouse. Physiol Behav. 2019 Jul 1;206:213-224. 4. Tyagi S, et al. Neuropharmacological Study on Capsaicin in Scopolamine-injected Mice. Curr Alzheimer Res. 2023;20(9):660-676. 5. Kagawa Y, et al. Investigation of capsaicin-induced superficial punctate keratopathy model due to reduced tear secretion in rats. Curr Eye Res. 2013 Jul;38(7):729-35. 6. Wang J, et al. Anti-inflammatory and retinal protective effects of capsaicin on ischaemia-induced injuries through the release of endogenous somatostatin. Clin Exp Pharmacol Physiol. 2017 Jul;44(7):803-814. 7. Xia J, Gu L, Guo Y, et al. Gut Microbiota Mediates the Preventive Effects of Dietary Capsaicin Against Depression-Like Behavior Induced by Lipopolysaccharide in Mice[J]. Frontiers in Cellular and Infection Microbiology. 2021, 11. 8. Tang K, Zhang X, Guo Y. Identification of the dietary supplement capsaicin as an inhibitor of Lassa virus entry[J]. Acta Pharmaceutica Sinica B. 2020.

TargetMolCitations

1. Lu J, Zhou W, Dou F, et al. TRPV1 sustains microglial metabolic reprogramming in Alzheimer's disease. EMBO reports. 2021, 22(6): e52013. 2. Lu J, Wang C, Cheng X, et al. A breakdown in microglial metabolic reprogramming causes internalization dysfunction of α-synuclein in a mouse model of Parkinson’s disease. Journal of Neuroinflammation. 2022, 19(1): 1-21 3. Wang C, Huang W, Lu J, et al. TRPV1-Mediated Microglial Autophagy Attenuates Alzheimer's Disease-Associated Pathology and Cognitive Decline. Frontiers in Pharmacology. 2021, 12: 763866-763866. 4. Wang C, Huang W, Lu J, et al. TRPV1-Mediated Microglial Autophagy Attenuates Alzheimer’s Disease-Associated Pathology and Cognitive Decline. Frontiers in Pharmacology. 2021, 12 5. Tang K, Zhang X, Guo Y. Identification of the dietary supplement capsaicin as an inhibitor of Lassa virus entry. Acta Pharmaceutica Sinica B. 2020 6. Xia J, Gu L, Guo Y, et al. Gut Microbiota Mediates the Preventive Effects of Dietary Capsaicin Against Depression-Like Behavior Induced by Lipopolysaccharide in Mice. Frontiers in Cellular and Infection Microbiology. 2021, 11. 7. Wang C, Lu J, Sha X, et al.TRPV1 regulates ApoE4-disrupted intracellular lipid homeostasis and decreases synaptic phagocytosis by microglia.Experimental & Molecular Medicine.2023: 1-17.

Related compound libraries

This product is contained In the following compound libraries:
Gut Microbial Metabolite Library Natural Product Library for HTS Anti-Cancer Approved Drug Library Anti-Cancer Clinical Compound Library Anti-Cancer Drug Library Membrane Protein-targeted Compound Library Natural Product Library Drug Repurposing Compound Library Traditional Chinese Medicine Monomer Library EMA Approved Drug Library

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Keywords

Capsaicin 404-86-4 Apoptosis Autophagy Membrane transporter/Ion channel TRP/TRPV Channel Qutenza 8-Methyl-N-vanillyl-trans-6-nonenamide Vanilloid (E)-Capsaicin Zostrix inhibitor inhibit

 

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