Cart
Endocrinology/Hormones Aromatase Aminoglutethimide

Aminoglutethimide

Catalog No. T1103   CAS 125-84-8
Synonyms: BA-16038, NSC-330915, DL-Aminoglutethimide

Aminoglutethimide, an aromatase inhibitor, is used in the therapy of advanced BREAST Y.

Aminoglutethimide, CAS 125-84-8
Pack Size Availability Price/USD Quantity
25 mg In stock 60.00
50 mg In stock 92.00
100 mg In stock 158.00
200 mg In stock 202.00
1 mL * 10 mM (in DMSO) In stock 50.00
Bulk Inquiry
Get quote
Select Batch  
Purity 98.81%
Purity 99.95%
Biological Description
Chemical Properties
Storage & Solubility Information
Preparing Solutions
Description Aminoglutethimide, an aromatase inhibitor, is used in the therapy of advanced BREAST Y.
Targets&IC50 Aromatase
Kinase Assay Concentration–response and kinetic studies: The microsomal protein (30 μg), [1β-3H]androstenedione (6.6 × 105 dpm) and NADPH (270 μM) are used for the concentration–response experiment with an incubation time of 20 minutes. The Aminoglutethimide is initially tested at 10 μM and 100 μM concentrations, followed by a full concentration–response study with at least 8 concentrations ranging from 0.01 μM to 160 μM. For the initial velocity study the concentration of [1β-3H]androstenedione is varied from 7.5 to 100 nM and the incubation time is set to 5 minutes. The tritiated water formed during the conversion of the tritiated substrate, [1β-3H]androstenedione, to estrone is quantified by liquid scintillation counting. Each assay is performed three times in duplicate and the results are treated by nonlinear regression analysis allowing the determination of the half-maximal inhibitory concentration (IC50).
Cell Research
The NCI-h295 tumor cell line is maintained in RPMI 1640 medium supplemented with transferrin (0.1 mg/mL), insulin (5 μg/mL), selenium (5.2 μg/mL) and 2% FCS. The cells are incubated for 48 hours with Aminoglutethimide (3, 30, 300 μM). Then cells are examined by trypan blue staining for cell viability, counted with a coulter counter. For the assessment of ACTH-R mRNA, cells are harvested, and total RNA is extracted, electrophoresed, blotted and hybridized with a human ACTH-R cDNA probe.(Only for Reference)
Cell lines: Adrenocortical tumor cell line NCI-h295
Synonyms BA-16038 , NSC-330915 , DL-Aminoglutethimide
Purity 98.81%
Molecular Weight 232.28
Formula C13H16N2O2
CAS No. 125-84-8

Storage

0-4℃ for short term (days to weeks), or -20℃ for long term (months).

Solubility Information

DMSO: 16 mg/mL (68.9 mM)

Ethanol: 7 mg/mL (30.13 mM)

( < 1 mg/ml refers to the product slightly soluble or insoluble )

Solution 1

1% DMSO+30% polyethylene glycol+1% Tween 80: 8 mg/mL

Citations

References and Literature
1. Neves MA, et al. Eur J Med Chem, 2009, 44(10), 4121-4127. 2. Fassnacht M, et al. J Endocrinol, 1998, 159(1), 35-42. 3. Santen RJ, et al. Cancer Res, 1982, 42(8 Suppl), 3353s-3359s. 4. Bastida CM, et al. Biochem Biophys Res Commun, 2001, 281(1), 244-248. 5. Kuma Y, et al. J Biol Chem. 2005, 280(20), 19472-19479.

Related compound libraries

This product is contained In the following compound libraries:
Approved Drug Library Bioactive Compound Library Inhibitor Library Anti-cancer Compound Library Endocrinology-Hormones Library FDA-approved Drug Library Anti-cancer Approved drug Library Killers Collection CNS-Penetrant Compound Library Anti-cancer Active Compound library Anti-cancer Drug library Drug Repurposing Library Target-Focused Phenotypic Screening Library Drug-induced Liver Injury (DILI) Compound Library NMPA-Approved Drug Library FDA Approved & Pharmacopeial Drug Library Anti-Breast Cancer Compound Library

Related Products

Related compounds with same targets
Anastrozole Androsta-1,4,6-triene-3,17-dione Formestane Aminoglutethimide Org30958 Exemestane Letrozole 3,4-Dimethoxyhydrocinnamic acid

Dose Conversion

Safe and effective drug dosing is necessary, regardless of its purpose of administration. Learn More

In vivo Formulation Calculator (Clear solution)

Step One: Enter information below
Dosage
mg/kg
Average weight of animals
g
Dosing volume per animal
ul
Number of animals
Step Two: Enter the in vivo formulation
% DMSO
%
% Tween 80
% ddH2O
Calculate Reset

Calculator

Molarity Calculator
Dilution Calculator
Reconstitution Calculation
Molecular Weight Calculator
=
X
X

Molarity Calculator allows you to calculate the

  • Mass of a compound required to prepare a solution of known volume and concentration
  • Volume of solution required to dissolve a compound of known mass to a desired concentration
  • Concentration of a solution resulting from a known mass of compound in a specific volume
See Example

An example of a molarity calculation using the molarity calculator
What is the mass of compound required to make a 10 mM stock solution in 10 ml of water given that the molecular weight of the compound is 197.13 g/mol?
Enter 197.13 into the Molecular Weight (MW) box
Enter 10 into the Concentration box and select the correct unit (millimolar)
Enter 10 into the Volume box and select the correct unit (milliliter)
Press calculate
The answer of 19.713 mg appears in the Mass box

X
=
X

Calculator the dilution required to prepare a stock solution

Calculate the dilution required to prepare a stock solution
The dilution calculator is a useful tool which allows you to calculate how to dilute a stock solution of known concentration. Enter C1, C2 & V2 to calculate V1.

See Example

An example of a dilution calculation using the Tocris dilution calculator
What volume of a given 10 mM stock solution is required to make 20ml of a 50 μM solution?
Using the equation C1V1 = C2V2, where C1=10 mM, C2=50 μM, V2=20 ml and V1 is the unknown:
Enter 10 into the Concentration (start) box and select the correct unit (millimolar)
Enter 50 into the Concentration (final) box and select the correct unit (micromolar)
Enter 20 into the Volume (final) box and select the correct unit (milliliter)
Press calculate
The answer of 100 microliter (0.1 ml) appears in the Volume (start) box

=
/

Calculate the volume of solvent required to reconstitute your vial.

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial.
Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

g/mol

Enter the chemical formula of a compound to calculate its molar mass and elemental composition

Tip: Chemical formula is case sensitive: C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:
To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.
Definitions of molecular mass, molecular weight, molar mass and molar weight:
Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed n the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

bottom

Tech Support

Answers to questions you may have can be found in the Inhibitor Handling Instructions. Topics include how to prepare stock solutions, how to store Products, and issues that need special attention for cell-based assays and animal experiments.