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Ac-DEVD-AMC

Catalog No. T18909 CAS 169332-61-0

Synonyms: AC-ASP-MET-GLN-ASP-AMC, AC-ASP-MET-GLN-ASP-7-AMINO-4-METHYLCOUMARIN

Ac-DEVD-AMC (AC-ASP-MET-GLN-ASP-7-AMINO-4-METHYLCOUMARIN) is the substrate of Caspase-3 .

All products from TargetMol are for Research Use Only. Not for Human or Veterinary or Therapeutic Use.

Ac-DEVD-AMC, CAS 169332-61-0

Pack Size	Availability	Price/USD
1 mg	In stock	$ 43.00
5 mg	In stock	$ 126.00
10 mg	In stock	$ 198.00
25 mg	In stock	$ 347.00
50 mg	In stock	$ 516.00
100 mg	In stock	$ 736.00

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Purity: 98.20%

Biological Description

Chemical Properties

Storage & Solubility Information

Description	Ac-DEVD-AMC (AC-ASP-MET-GLN-ASP-7-AMINO-4-METHYLCOUMARIN) is the substrate of Caspase-3 .
In vitro	The AMC moiety is highest at 2 h with an area?of 5.6±1.8, the AMC peak area for Ac-DEVD-AMC incubated without a lung specimen (substrate alone) for 6 h is relatively small [1]. Ac-DEVD-AMC can be used to monitor intracellular caspase-3 activity[2].
Cell Research	Lung fragments (about 20 mg each) are collected from rats and mice. The samples are incubated at 37°C in 50 mL KH buffer or MEM (continuously gass with 95% O2: 5% CO2) for up to 6 h. At specific time points, samples are incubated in oxygenated KH buffer or MEM with 32 μM zVAD-fmk or 15 μL DMSO for 20 min (f/v=1.0 mL). Ac-DEVD-AMC (37 μM) is then added and the incubation continues for an additional 20 min. At the end of incubation, the tissue is disrupted by vigorous homogenization for 2 min, sonication for 3 min, and 10 passages through a 27-G needle. This disruption procedure quenches the Ac-DEVD-AMC cleavage reaction due to dilution. The supernatants are collected by centrifugation (~6,300g for 90 min) through a microcentrifuge filter, separated on HPLC, and analyzed for the fluorogenic AMC moiety [2].

Synonyms	AC-ASP-MET-GLN-ASP-AMC, AC-ASP-MET-GLN-ASP-7-AMINO-4-METHYLCOUMARIN
Molecular Weight	675.64
Formula	C30H37N5O13
CAS No.	169332-61-0

Storage

keep away from moisture

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: ≤ 10 mg/mL (14.80 mM)

H2O: < 0.1 mg/mL (insoluble)

References and Literature

1. Ahmed R Alsuwaidi, et al. Lung tissue bioenergetics and caspase activity in rodents. BMC Res Notes. 2013; 6: 12. 2. Ahmed R Alsuwaidi, et al. Bioenergetics of murine lungs infected with respiratory syncytial virus. Virol J. 2013; 10: 22.

Related compound libraries

This product is contained In the following compound libraries：

Peptide Compound Library Anti-Neurodegenerative Disease Compound Library Apoptosis Compound Library Bioactive Compounds Library Max Pyroptosis Compound Library Bioactive Compound Library

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

Ac-DEVD-AMC 169332-61-0 Apoptosis Proteases/Proteasome Caspase Ac DEVD AMC AcDEVDAMC Inhibitor inhibit AC-ASP-MET-GLN-ASP-AMC AC-ASP-MET-GLN-ASP-7-AMINO-4-METHYLCOUMARIN inhibitor

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