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9-Methoxycamptothecin

Name: 9-Methoxycamptothecin
Brand: TargetMol
SKU: T5S1952
Price: 48 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T5S1952Cas No. 39026-92-1

9-Methoxycamptothecin (MCPT) has antitumour activities through topoisomerase inhibition.

9-Methoxycamptothecin

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Purity: 99.56%

Catalog No. T5S1952Cas No. 39026-92-1

9-Methoxycamptothecin (MCPT) has antitumour activities through topoisomerase inhibition.

Pack Size	Price	USA Warehouse	Global Warehouse	Quantity
25 mg	$48	-	In Stock
50 mg	$72	-	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Batch Information

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Purity:99.56%

Appearance:Solid

Color:White to Yellow

Resource Download

COA LCMS HNMR

Product Introduction

Bioactivity

Description

9-Methoxycamptothecin (MCPT) has antitumour activities through topoisomerase inhibition.

In vitro

9-Methoxycamptothecin(MCPT) induced cytotoxicity in seven human cancer cell lines in a dose dependent manner after 72h, with A2780 and Hela cell lines more sensitive, so the two cell lines were chosen to do further studies. MCPT induced strong G2/M arrest in both A2780 cells and Hela cells after 24h, following by substantial sub-G1 arrest (indicating apoptosis). The apoptosis was verified by staining with Annexin V-FITC and propidium iodide. ROS generation increased significantly in MCPT-induced apoptosis. Meanwhile, the apoptosis appeared to be dependent on caspase-3, -8 and -9 in A2780 cells, and caspase-3 in Hela cells. In addition, MCPT induced up-regulation expression of most of seventeen genes in both cell lines. Western blot verified that changes of TNFα, Fas, P53 and P27 protein level were consistent with their gene expression changes[1].

Chemical Properties

Molecular Weight	378.38
Formula	C₂₁H₁₈N₂O₅
Cas No.	39026-92-1
Smiles	CC[C@@]1(O)C(=O)OCc2c1cc1-c3nc4cccc(OC)c4cc3Cn1c2=O
Relative Density.	1.5g/cm3

Storage & Solubility Information

Storage

In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 50 mg/mL (132.14 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+90% Corn Oil: 1 mg/mL (2.64 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.6428 mL	13.2142 mL	26.4285 mL	132.1423 mL
5 mM	0.5286 mL	2.6428 mL	5.2857 mL	26.4285 mL
10 mM	0.2643 mL	1.3214 mL	2.6428 mL	13.2142 mL
20 mM	0.1321 mL	0.6607 mL	1.3214 mL	6.6071 mL
50 mM	0.0529 mL	0.2643 mL	0.5286 mL	2.6428 mL
100 mM	0.0264 mL	0.1321 mL	0.2643 mL	1.3214 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc