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7-Hydroxymethotrexate

Catalog No. T20339 CAS 5939-37-7

Synonyms: NSC 380963, NSC-380963, NSC380962, NSC380963, NSC 380962, NSC-380962

7-Hydroxymethotrexate is the major metabolite of Methotrexate.Methotrexate is an inhibitor of folic acid, inhibiting dihydrofolate reductase, which prevents the conversion of folic acid to tetrahydrofolate, and inhibiting DNA synthesis.Methotrexate is also an inhibitor of metabolism.

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7-Hydroxymethotrexate Chemical Structure

7-Hydroxymethotrexate, CAS 5939-37-7

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1 mg	In stock	$ 312.00
5 mg	In stock	$ 749.00
10 mg	In stock	$ 987.00
25 mg	In stock	$ 1,530.00
50 mg	In stock	$ 2,450.00

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Biological Description

Chemical Properties

Storage & Solubility Information

Description	7-Hydroxymethotrexate is the major metabolite of Methotrexate.Methotrexate is an inhibitor of folic acid, inhibiting dihydrofolate reductase, which prevents the conversion of folic acid to tetrahydrofolate, and inhibiting DNA synthesis.Methotrexate is also an inhibitor of metabolism.
In vitro	With an affinity for DHFR markedly lower (>100-fold) than Methotrexate (MTX), 7-Hydroxymethotrexate (7-OHMTX)[1].
In vivo	Administered intravenously at a dose of 4 mg/kg, 7-Hydroxymethotrexate (7-OHMTX) exhibits a terminal half-life of 97.2 min and a total clearance value of 9.6 mL/min•mg[1].

Synonyms	NSC 380963, NSC-380963, NSC380962, NSC380963, NSC 380962, NSC-380962
Molecular Weight	470.44
Formula	C20H22N8O6
CAS No.	5939-37-7

Storage

store at low temperature

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: Soluble

References and Literature

1. L Fahrig, et al. Pharmacokinetics of methotrexate (MTX) and 7-hydroxymethotrexate (7-OH-MTX) in rats and evidence for the metabolism of MTX to 7-OH-MTX. Cancer Chemother Pharmacol. 1989;23(3):156-60. 2. Ping Guo, et al. Determination of methotrexate and its major metabolite 7-hydroxymethotrexate in mouse plasma and brain tissue by liquid chromatography-tandem mass spectrometry. J Pharm Biomed Anal. 2007 Apr 11;43(5):1789-95. 3. Białk-Bielińska A, Mulkiewicz E, Stokowski M, Stolte S, Stepnowski P. Acute aquatic toxicity assessment of six anti-cancer drugs and one metabolite using biotest battery - Biological effects and stability under test conditions. Chemosphere. 2017 Dec;189:689-698. doi: 10.1016/j.chemosphere.2017.08.174. Epub 2017 Sep 21. PubMed PMID: 28968575. 4. Patel H, Giri P, Ghoghari A, Delvadia P, Syed M, Srinivas NR. Review of the bioanalytical methods for the determination of methotrexate and its metabolites in in vitro, preclinical and clinical studies: Case studies and perspectives. Biomed Chromatogr. 2017 Jan;31(1). doi: 10.1002/bmc.3849. Epub 2016 Oct 17. Review. PubMed PMID: 27623319.

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Keywords

7-Hydroxymethotrexate 5939-37-7 Metabolism Drug Metabolite NSC 380963 NSC-380963 NSC380962 NSC380963 NSC 380962 NSC-380962 inhibitor inhibit

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