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20(S)-Hydroxycholesterol

Name: 20(S)-Hydroxycholesterol
Brand: TargetMol
SKU: T10085
Price: 30 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T10085Cas No. 516-72-3

Alias 20α-Hydroxycholesterol

20(S)-Hydroxycholesterol (20α-Hydroxycholesterol) is an allosteric activator of the oncoprotein smoothened (Smo).

20(S)-Hydroxycholesterol

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Purity: 99.79%

Catalog No. T10085Alias 20α-HydroxycholesterolCas No. 516-72-3

20(S)-Hydroxycholesterol (20α-Hydroxycholesterol) is an allosteric activator of the oncoprotein smoothened (Smo).

Pack Size	Price	USA Warehouse	Global Warehouse
1 mg	$30	-	In Stock
5 mg	$76	-	In Stock
10 mg	$128	-	In Stock
25 mg	$278	-	In Stock
50 mg	$455	-	In Stock
100 mg	$672	-	In Stock
1 mL x 10 mM (in DMSO)	$84	-	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

This molecule is a custom-made product. TargetMol has an excellent synthesis team with the experience and capability to provide you with cost-effective products. However, due to objective factors, there is a small probability that the synthesis may not be successful during the R&D process. We appreciate your understanding. If you have any questions, please feel free to contact us. We are committed to serving you wholeheartedly.

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Batch Information

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Purity:99.79%

Resource Download

COA LCMS HNMR

Product Introduction

Bioactivity

Description	20(S)-Hydroxycholesterol (20α-Hydroxycholesterol) is an allosteric activator of the oncoprotein smoothened (Smo).
In vitro	20(S)-Hydroxycholesterol activates the hedgehog signaling pathway with an EC50 of 3 μM in NIH3T3 cells[2].
Synonyms	20α-Hydroxycholesterol

Chemical Properties

Molecular Weight	402.65
Formula	C₂₇H₄₆O₂
Cas No.	516-72-3
Smiles	C[C@@]12[C@]([C@]3([C@@]([C@]4(C)C(=CC3)C[C@@H](O)CC4)(CC1)[H])[H])(CC[C@@]2([C@@](CCCC(C)C)(C)O)[H])[H]
Relative Density.	1.03g/cm3

Storage & Solubility Information

Storage

store under nitrogen | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

Ethanol: 50 mg/mL (124.18 mM), Sonication is recommended.

DMSO: 27 mg/mL (67.06 mM), Sonication and heating to 60℃ are recommended.

In Vivo Formulation

10% DMSO+90% Corn Oil: 1 mg/mL (2.48 mM), Sonication is recommeded.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO/Ethanol

	1mg	5mg	10mg	50mg
1 mM	2.4835 mL	12.4177 mL	24.8355 mL	124.1773 mL
5 mM	0.4967 mL	2.4835 mL	4.9671 mL	24.8355 mL
10 mM	0.2484 mL	1.2418 mL	2.4835 mL	12.4177 mL
20 mM	0.1242 mL	0.6209 mL	1.2418 mL	6.2089 mL
50 mM	0.0497 mL	0.2484 mL	0.4967 mL	2.4835 mL

Ethanol

	1mg	5mg	10mg	50mg
100 mM	0.0248 mL	0.1242 mL	0.2484 mL	1.2418 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:

For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . TargetMol | Animal experiments

A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent

DMSO+30% PEG300+5% Tween 80+60% Saline/PBS/ddH₂O. So your working solution concentration is 2 mg/mL。

Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSO TargetMol | reagent

(mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.

Preparation method for in vivo formula: Take 50 μL DMSO TargetMol | reagent

main solution, add 300 μLPEG300 TargetMol | reagent

mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLSaline/PBS/ddH₂O TargetMol | reagent

mix well and clarify

For Reference Only. Please develop an appropriate dissolution method based on your laboratory animals and route of administration.

All types of co-solvents required for the protocol, such asDMSO, PEG300/ PEG400, Tween 80, SBE-β-CD, corn oil are available for purchase on the TargetMol website with a simple click.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc