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(Z)-Mirin

Catalog No. T3134 CAS 1198097-97-0

Synonyms: Mirin

(Z)-Mirin is a potent Mre11–Rad50–Nbs1 (MRN) complex inhibitor, and inhibits Mre11-associated exonuclease activity.

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(Z)-Mirin, CAS 1198097-97-0

Pack Size	Availability	Price/USD
5 mg	In stock	$ 50.00
10 mg	In stock	$ 79.00
25 mg	In stock	$ 143.00
50 mg	In stock	$ 238.00
100 mg	In stock	$ 438.00
200 mg	In stock	$ 571.00
500 mg	In stock	$ 917.00
1 mL * 10 mM (in DMSO)	In stock	$ 50.00

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Purity: 99.96%

Purity: 98.37%

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Description	(Z)-Mirin is a potent Mre11–Rad50–Nbs1 (MRN) complex inhibitor, and inhibits Mre11-associated exonuclease activity.
In vitro	Mirin inhibits DSB-induced ATM activation, the ATM-dependent phosphorylation of the downstream targets Nbs1 and Chk2 and the MRN-dependent autophosphorylation of ATM at Ser1981 in response to DSBs. Mirin also inhibits the G2 checkpoint in TOSA4 cells, and homology-dependent DNA repair in HEK293 cells. [1] In cells with integrated HPV16 (SiHa), Mirin sensitizes HPV episomes to PA25 resulting in a ～5-fold reduction of the PA25 IC50. [2] Pretreatment with mirin also decreases cell viability and inhibits proliferating cell nuclear antigen expression in cisplatin-treated human embryonic kidney 293 cells. [3]
Kinase Assay	Nuclease assay: Reactions with oligonucleotide nonhairpin substrates contains 25 mM MOPS (pH 7.0), 60 mM KCl, 0.2% Tween 20, 2 mM DTT, 1 mM or 5 MnCl2 (or 5 mM MgCl2, or 5 mM CaCl2), 0.1 pmol of DNA substrate, and 0.3 pmol of Mre11 (or an equivalent amount of Mre11 complexed with Rad50) in a volume of 10 μl, and are incubated at 37°C for 30 min. SDS, EDTA, and proteinase K are then added to final concentrations of 0.2%, 5 mM, and 0.1 mg/ml, respectively, and incubated for another 15 min. 4 μl of each reaction is mixed with 4 μl of formamide loading buffer, and then loaded onto a sequencing gel containing 10% acrylamide and 7 M urea. After the run, each gel is analyzed using a phosphorimaging system. Reactions containing hairpin substrates are identical to those with nonhairpin substrates except that 3 pmol of Mre11 is added to reactions as indicated, and the reactions are incubated at room temperature overnight. Nonhomologous end-joining reactions contains 25 mM MOPS (pH 7.0), 60 mM KCl, 0.2% Tween 20, 2 mM DTT, 4 mM MgCl2, 2 mM MnCl2, 0.5 mM ATP, 4 ng of plasmid DNA, 10% polyethylene glycol, 0.01 pmol of human DNA ligase I, and 0.06 pmol of Mre11 or 0.1 units of E. coli exonuclease III (GIBCO-BRL), in a volume of 10 μl. After incubation at 37°C for 25 min, Tween 20 is added to a final concentration of 0.5%, and a 2.5 μl aliquot is amplified by PCR using primers DAR5 and DAR147. PCR products are cloned using the TA cloning kit and sequenced using an automated ABI Capillary Genetic Analyzer.
Cell Research	Human embryonic kidney (HEK) 293 cells are maintained in RPMI-1640 supplemented with 5% heat-inactivated fetal bovine serum, penicillin (100 U/mL), and streptomycin (100 mg/mL) in humidified air with 5% CO2 at 37 °C. Cells are given fresh medium at 48 h intervals. The cells are seeded in 96-well plates in regular growth medium. Cells are pretreated with mirin (100 μM for 1 h before the cisplatin (20 μM) treatment followed by incubation for 8 and 24 h. The MTT assay is performed using the EZ-Cytox cell viability assay kit according to the manufacturer's protocol and MTT reduction is measured at a 450 nm wavelength using a micro-plate reader. (Only for Reference)

Synonyms	Mirin
Molecular Weight	220.25
Formula	C10H8N2O2S
CAS No.	1198097-97-0

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 22 mg/mL (100 mM)

References and Literature

1. Dupré A, et al. Nat Chem Biol. 2008, 4(2), 119-125. 2. Edwards TG, et al. PLoS One. 2013, 8(10):e75406. 3. Kim YJ, et al. Environ Toxicol Pharmacol. 2015, 40(1), 12-17.

Related compound libraries

This product is contained In the following compound libraries：

Kinase Inhibitor Library Inhibitor Library Reprogramming Compound Library Anti-Colorectal Cancer Compound Library Anti-Prostate Cancer Compound Library DNA Damage & Repair Compound Library Bioactive Lipid Compound Library Oxidation-Reduction Compound Library Anti-Pancreatic Cancer Compound Library Cancer Cell Differentiation Compound Library

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Keywords

(Z)-Mirin 1198097-97-0 DNA Damage/DNA Repair PI3K/Akt/mTOR signaling ATM/ATR Mirin Inhibitor (Z)Mirin (Z) Mirin inhibit inhibitor

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