TIGIT Protein, Human, Recombinant (aa 1-141, His)

TIGIT Protein, Human, Recombinant (aa 1-141, His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 14.5 kDa and the accession number is Q495A1-1.

Pack Size	Availability	Price/USD	Quantity
50 μg	5 days	$ 357.00
500 μg	5 days	$ 2,330.00

Pack Size

Availability

Price/USD

Quantity

50 μg

5 days

$ 357.00

500 μg

5 days

$ 2,330.00

Description

TIGIT Protein, Human, Recombinant (aa 1-141, His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 14.5 kDa and the accession number is Q495A1-1.

Species	Human
Expression System	HEK293 Cells
Tag	C-His
Accession Number	Q495A1-1
Synonyms	T cell immunoreceptor with Ig and ITIM domains, VSIG9, VSTM3, WUCAM
Construction	The Human TIGIT (Q495A1-1) (Met1-Pro141) was expressed with a polyhistidine tag at the C-terminus.
Protein Purity	> 90 % as determined by SDS-PAGE.>90% as determined by SEC-HPLC
Molecular Weight	14.5 kDa (predicted)

Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	TIGIT, also known as V-set and transmembrane domain-containing protein 3 (VSTM3) or V-set and immunoglobulin domain-containing protein 9 (VSIG9) is a new surface protein containing an immunoglobulin variable domain, a transmembrane domain and an immunoreceptor tyrosine-based inhibitory motif (ITIM). TIGIT is expressed on regulatory, memory, activated T cells and NK cells. It binds PVR with high affinity, and PVRL2 with lower affinity, but not PVRL3. Knockdown of TIGIT with siRNA in human memory T cells did not affect T cell responses, however, TIGIT inhibits NK cytotoxicity directly through its ITIM. TIGIT suppresses T cell activation by promoting the generation of mature immunoregulatory dendritic cells. The binding of PVR to TIGIT on human dendritic cells enhanced the production of IL-1 and diminished the production of IL-12p4. Also, TIGIT counter inhibits the NK-mediated killing of tumor cells and protects normal cells from NK-mediated cytotoxicity thus providing an "alternative self" mechanism for MHC class I inhibition.Cancer ImmunotherapyCo-inhibitory Immune Checkpoint TargetsImmune CheckpointImmune Checkpoint TargetsImmunotherapyTargeted Therapy