P-Selectin Protein, Mouse, Recombinant (His)

Name: P-Selectin Protein, Mouse, Recombinant (His)
Brand: TargetMol
SKU: TMPY-03702
Price: 68 USD
Availability: InStock
Rating: 5 (10 reviews)

(Synonyms: selectin P (granule membrane protein 140kDa, antigen CD62), PADGEM, LECAM3, Grmp, GMP-140, CD62P) Copy Product Info

Synonyms: selectin P (granule membrane protein 140kDa, antigen CD62), PADGEM, LECAM3, Grmp, GMP-140, CD62P

Catalog No. TMPY-03702 Copy Product Info

Purity: 95%

P-Selectin Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 50.9 kDa and the accession number is Q01102.

Pack Size	Price	USA Stock	Global Stock
5 µg	$68	-	In Stock
10 µg	$108	-	In Stock
20 µg	$178	7-10 days	7-10 days
50 µg	$359	7-10 days	7-10 days
100 µg	$696	7-10 days	7-10 days
200 µg	$1,060	7-10 days	7-10 days
500 µg	$2,230	7-10 days	7-10 days
1 mg	$4,530	7-10 days	7-10 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

Add to Cart

Add to Quotation

For research use only—not for human use. No sales to individuals. Use as intended only.

Questions

Batch Information

Select Batch

Purity:95%

Appearance:Lyophilized powder

Color:White

Resource Download

COA User Guide of Recombinant Proteins

Product Information

Bioactivity

Bioactivity	Measured by the ability of the immobilized protein to support the adhesion of U937 cells. When 5 x 10E4 cells/well are added to SELP-coated plates (5 μg/mL and 100 μL/well), approximately >60% cells will adhere specifically after 60 minutes at 37℃.
Description	P-Selectin Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 50.9 kDa and the accession number is Q01102.
Species	Mouse
Expression System	HEK293 Cells
Tag	C-His
Accession Number	Q01102
Construction	A DNA sequence encoding the mouse SELP (Q01102) (Met1-Ala709) was expressed with a C-terminal polyhistidine tag. Predicted N terminal: Trp 42
Protein Purity	> 95 % as determined by SDS-PAGE
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Synonyms	selectin P (granule membrane protein 140kDa, antigen CD62), PADGEM, LECAM3, Grmp, GMP-140, CD62P
Research Background	P selectin (SELP) is a 140kDa protein that is stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. SELP mediates rapid rolling of leukocyte rolling over vascular surfaces during the initial steps in inflammation through interaction with PSGL1. P selectin is a cell adhesion molecule on the surface of activated endothelial cells. Cellular adhesion molecules are a large family of proteins that attach the cytoskeleton and intracellular signaling cascades with the extracellular environment. SELP is a calcium-dependent receptor for myeloid cells that binds to sialylated forms of Lewis blood group carbohydrate antigens on neutrophils and monocytes. This protein redistributes to the plasma membrane during platelet activation and degranulation and mediates the interaction of activated endothelial cells or platelets with leukocytes.

Chemical Properties

Molecular Weight

74 kDa (predicted); 116 kDa (reducing conditions)

Storage & Solubility Information

Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.