NAA10/ARD1 Protein, Human, Recombinant (His & GST)

NAA10/ARD1 Protein, Human, Recombinant (His & GST) is expressed in Baculovirus insect cells with His and GST tag. The predicted molecular weight is 54 kDa and the accession number is P41227.

Pack Size	Availability	Price/USD	Quantity
100 μg	5 days	$ 600.00

Pack Size

Availability

Price/USD

Quantity

100 μg

5 days

$ 600.00

Description

NAA10/ARD1 Protein, Human, Recombinant (His & GST) is expressed in Baculovirus insect cells with His and GST tag. The predicted molecular weight is 54 kDa and the accession number is P41227.

Species	Human
Expression System	Baculovirus-Insect Cells
Tag	His,GST
Accession Number	P41227
Synonyms	ARD1P, DXS707, ARD1, NATD, ARD1A, N(alpha)-acetyltransferase 10, NatA catalytic subunit, MCOPS1, TE2, OGDNS, NAA10, N(α)-acetyltransferase 10, NatA catalytic subunit
Construction	A DNA sequence encoding the human ARD1A (P41227) (Met1-Ser235) was expressed with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Protein Purity	> 95 % as determined by SDS-PAGE
Molecular Weight	54 kDa (predicted)

Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Formulation	Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.4, 10% glycerol. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0. 01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Reconstitution	A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Stability & Storage	Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Shipping	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Research Background	ARD1 is a member of the 2-kDa ARF protein family. It is a multifunctional protein. ARD1 has an 18-kDa ADP-ribosylation factor (ARF) domain at the C-terminus (amino acids 43-574), and a 46-kDa N-terminal domain (amino acids 1-42). The C-terminal region of ARD1 may be involved in the formation of both ARD1-ARD1 and ARD1-NAT1 complexes. ARD1 and NAT1 genes are required for the expression of an N-terminal protein acetyltransferase. This activity is required for full repression of the silent mating-type locus HML, for sporulation, and for entry into G. Recombinant ARD1 (amino acids 1-574) or its RING finger domain (amino acids 1-11) produced polyubiquitylated proteins when incubated in vitro with a mammalian E1, an E2 enzyme, ATP, and ubiquitin.