MFGE8 Protein, Human, Recombinant (His)

Name: MFGE8 Protein, Human, Recombinant (His)
Brand: TargetMol
SKU: TMPY-02836
Price: 43 EUR
Availability: InStock
Rating: 5 (10 reviews)

(Synonyms: SPAG10, SED1, OAcGD3S, milk fat globule-EGF factor 8 protein, MFGM, MFG-E8, HsT19888, hP47, HMFG, EDIL1, BA46) Copy Product Info

Synonyms: SPAG10, SED1, OAcGD3S, milk fat globule-EGF factor 8 protein, MFGM, MFG-E8, HsT19888, hP47, HMFG, EDIL1, BA46

Catalog No. TMPY-02836 Copy Product Info

Purity: 95.7%

MFGE8 Protein, Human, Recombinant (His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 42 kDa and the accession number is Q08431-1.

Pack Size	Price	EUR Stock	Global Stock
5 µg	43 €	-	In Stock
10 µg	67 €	-	In Stock
20 µg	106 €	-	In Stock
50 µg	206 €	-	In Stock
100 µg	347 €	-	In Stock
200 µg	594 €	7-10 days	7-10 days
500 µg	1.197 €	7-10 days	7-10 days

For In stock only · Estimated delivery: EUR Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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Purity:95.7%

Appearance:Lyophilized powder

Color:White

Resource Download

COA User Guide of Recombinant Proteins

Product Information

Bioactivity

Bioactivity	When 5 x 10E4 cells/well are added to Recombinant Human MFG-E8 coated plates (12.5 μg/mL, 100 μL/well), 45-85% cells will adhere after 1 hour at 37℃.
Description	MFGE8 Protein, Human, Recombinant (His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 42 kDa and the accession number is Q08431-1.
Species	Human
Expression System	Baculovirus Insect Cells
Tag	C-His
Accession Number	Q08431-1
Construction	A DNA sequence encoding the human MFGE8 isoform 1 (Q08431-1) (Met 1-Cys 387) was fused with a polyhistidine tag at the C-terminus. Predicted N terminal: Leu 24
Protein Purity	≥ 80 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC. ,
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM Tris, 500 mM NaCl, pH 7.4, 10% gly. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	Reconstituted with sterile deionized water to 0.25 mg/mL. Reconstitution conditions may vary depending on the lot.
Synonyms	SPAG10, SED1, OAcGD3S, milk fat globule-EGF factor 8 protein, MFGM, MFG-E8, HsT19888, hP47, HMFG, EDIL1, BA46
Research Background	MFG-E8, also known as lactadherin and MFGE8, contains 1 EGF-like domain and 2 F5/8 type C domains. It also contains phosphatidylserine (PS) binding domain, as well as an Arginine-Glycine-Aspartic acid motif, which enables the binding to integrins. It binds PS, which is exposed on the surface of apoptotic cells. MFG-E8 is expressed in mammary epithelial cell surfaces and aortic media. Overexpression of MFG-E8 can be found in several carcinomas. MFG-E8 has opsonization of the apoptotic cells and binding to integrins on the surface of phagocytic cells. It also mediates the engulfment of the dead cell. MFG-E8 plays an important role in the maintenance of intestinal epithelial homeostasis and the promotion of mucosal healing. It promotes VEGF-dependent neovascularization and contributes to the phagocytic removal of apoptotic cells in many tissues. It also binds to phosphatidylserine-enriched cell surfaces in a receptor-independent manner.

Chemical Properties

Molecular Weight

42 kDa (predicted); 45 kDa (reducing conditions)

Storage & Solubility Information

Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.