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MFGE8 Protein, Human, Recombinant (His & Avi), Biotinylated
(Synonyms: SPAG10, SED1, OAcGD3S, milk fat globule-EGF factor 8 protein, MFGM, MFG-E8, HsT19888, hP47, HMFG, EDIL1, BA46) Copy Product InfoSynonyms: SPAG10, SED1, OAcGD3S, milk fat globule-EGF factor 8 protein, MFGM, MFG-E8, HsT19888, hP47, HMFG, EDIL1, BA46
Catalog No. TMPY-05235 Copy Product Info
MFGE8 Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in Baculovirus insect cells with His and Avi tag. The predicted molecular weight is 44.1 kDa and the accession number is Q08431.2.
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 5 µg | $167 | 7-10 days | 7-10 days | |
| 10 µg | $277 | 7-10 days | 7-10 days | |
| 20 µg | $462 | 7-10 days | 7-10 days | |
| 50 µg | $746 | 7-10 days | 7-10 days | |
| 100 µg | $1,230 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Information
Bioactivity
Chemical Properties
Storage & Solubility Information
| Bioactivity | Activity testing is in progress. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first. |
| Description | MFGE8 Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in Baculovirus insect cells with His and Avi tag. The predicted molecular weight is 44.1 kDa and the accession number is Q08431.2. |
| Species | Human |
| Expression System | Baculovirus Insect Cells |
| Tag | His, Avi |
| Accession Number | Q08431.2 |
| Construction | A DNA sequence encoding the human MFGE8 (Q08431.2) (Met1-Cys387) was expressed with a c-terminal polyhistidine tagged AVI tag at the C-terminus. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed. Predicted N terminal: Leu 24 |
| Protein Purity | > 95 % as determined by SDS-PAGE. |
| Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
| Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM Tris, 150 mM NaCl, pH 8.5, 10% glycerol, 0.2 M Arginine, 0.01% Tween-20.Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
| Reconstitution | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
| Synonyms | SPAG10, SED1, OAcGD3S, milk fat globule-EGF factor 8 protein, MFGM, MFG-E8, HsT19888, hP47, HMFG, EDIL1, BA46 |
| Research Background | MFG-E8, also known as lactadherin and MFGE8, contains 1 EGF-like domain and 2 F5/8 type C domains. It also contains phosphatidylserine (PS) binding domain, as well as an Arginine-Glycine-Aspartic acid motif, which enables the binding to integrins. It binds PS, which is exposed on the surface of apoptotic cells. MFG-E8 is expressed in mammary epithelial cell surfaces and aortic media. Overexpression of MFG-E8 can be found in several carcinomas. MFG-E8 has opsonization of the apoptotic cells and binding to integrins on the surface of phagocytic cells. It also mediates the engulfment of the dead cell. MFG-E8 plays an important role in the maintenance of intestinal epithelial homeostasis and the promotion of mucosal healing. It promotes VEGF-dependent neovascularization and contributes to the phagocytic removal of apoptotic cells in many tissues. It also binds to phosphatidylserine-enriched cell surfaces in a receptor-independent manner. |
| Molecular Weight | 44.1 kDa (predicted) |
| Shipping | In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice. |
| Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
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Related Tags: MFGE8 Protein, Human, Recombinant (His & Avi), Biotinylated chemical structure | MFGE8 Protein, Human, Recombinant (His & Avi), Biotinylated molecular weight
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