HAI-2/SPINT2 Protein, Mouse, Recombinant (His)

Catalog No. TMPY-00926

HAI-2/SPINT2 Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 14 kDa and the accession number is Q9WU03-2.

HAI-2/SPINT2 Protein, Mouse, Recombinant (His)

Catalog No. TMPY-00926

HAI-2/SPINT2 Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 14 kDa and the accession number is Q9WU03-2.

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50 μg	$600	7-10 days

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Product Information

Biological Activity	Activity testing is in progress. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
Description	HAI-2/SPINT2 Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 14 kDa and the accession number is Q9WU03-2.
Species	Mouse
Expression System	HEK293 Cells
Tag	C-His
Accession Number	Q9WU03-2
Synonyms	serine peptidase inhibitor, Kunitz type, 2,HAI-2,C76321,AL024025
Construction	A DNA sequence encoding the extracellular domain (Met 1-Lys 140) of mouse SPINT2 (NP_001076017.1) precursor was expressed with a C-terminal polyhistidine tag. Predicted N terminal: Glu 31
Protein Purity	> 90 % as determined by SDS-PAGE
Molecular Weight	14 kDa (predicted); 22 kDa (reducing condition, due to glycosylation)
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	Choanal (CA) and gastrointestinal atresias (GA) are an important feature of syndromic congenital sodium diarrhea (sCSD), a disorder recently associated with mutations in the gene for serine protease inhibitor type 2 (SPINT2). The SPINT2 gene is epigenetically silenced or downregulated in human cancers, altering the balance of HGF activation/inhibition ratio, which contributes to cancer development and progression. SPINT2 is a tumor suppressor gene that inhibits proteases implicated in cancer progression, like HGFA, hepsin and matriptase. Loss of SPINT2 expression in tumors has been associated with gene promoter hypermethylation. SPINT2 (serine peptidase inhibitor Kunitz type 2), a proteolytic inhibitor of hepatocyte growth factor activator (HGFA), has a significant role in the suppression of the HGF-MET pathway and malignant melanoma progression.