1. Immobilized Human GPRC5D Protein at 5 μg/mL (100 μL/well) can bind Anti-Human GPRC5D Antibody, Human IgG1, the EC50 is 20.6ng/mL（QC tested). 2.Loaded Anti-GPRC5D Antibody on Protein A Biosensor, can bind GPRC5D Protein with an affinity constant of 0.782 nM as determined in BLI assay (Sartorius Octet Red96e) (Routinely tested). 3.Captured Anti-GPRC5D antibody  on anti-Human IgG Fc via CM5 Chip can bind GPRC5D protein with an affinity constant of 9.609nM as determined in a SPR assay  (Routinely tested).

GPRC5D Protein, Human, Recombinant (His & FLAG) is expressed in HEK293 mammalian cells with His and Flag tag. The predicted molecular weight is 32.83 kDa and the accession number is Q9NZD1.

Human

HEK293 Cells

GPRC5D

A DNA sequence encoding the human GPRC5D (Q9NZD1) (Met1-Ser268) was expressed, with a Flag tag at the N-terminus and a polyhistidine tag at the C-terminus.Nanodisc is a versatile tool for studying membrane proteins. Using styrene-maleic acid (SMA) copolymer, membrane proteins can be extracted directly from prokaryotic and eukaryotic expression systems in the absence of detergents to preserve the protein structure and function better. Compared to membrane scaffold proteins (MSPs) nanodiscs, SMA nanodiscs also have the advantage of preserving proteins' nature by maintaining native lipids surrounded without introducing any heterologous proteins, which allows studies of protein structure and functions in a native-like environment. Predicted N terminal: Met

≥ 75 % as determined by SDS-PAGE.

It is recommended to store the product under sterile conditions at -70°C or lower. Samples are stable for up to 3 months at -80°C. Please avoid multiple freeze-thaw cycles and store products in aliquots.

Orphan G-protein-coupled receptor family C group 5 member D (GPRC5D) has seven transmembrane segments forming the core of the receptor, as well as an intracellular C-tail and an extracellular N-terminal domain. GPRC5D is highly and selectively expressed on the surface of CD138+ multiple myeloma (MM) cells, whereas in normal tissues, a much lower expression is limited to plasma cells and certain hard keratinizing tissues. This makes GPRC5D a promising marker for monitoring tumor load and a target antigen for MM-specific T cell-engaging therapies such as bispecific antibodies and CAR-T cells.