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FCGRT & B2M Heterodimer Protein, Rat, Recombinant (His)

(Synonyms: Fc fragment of IgG, receptor, transporter, α, Fc fragment of IgG, receptor, transporter, alpha) Copy Product Info

Synonyms: Fc fragment of IgG, receptor, transporter, α, Fc fragment of IgG, receptor, transporter, alpha

Catalog No. TMPY-02829 Copy Product Info
Purity: 99%
FCGRT & B2M Heterodimer Protein, Rat, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 44 kDa and the accession number is P13599&P07151.

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Purity:99%
Appearance:Lyophilized powder
Color:White
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Product Information

Bioactivity
Bioactivity
Recombinant Rat FCGRT & B2M Heterodimer Protein (Cat#TMPY-02829) captured on NTA chip, can bind anti-ERBB2 (Trastuzumab biosimilar) with an affinity constant of 1.310 nM as determined in an SPR assay (Biacore 8K) (QC tested).
Description
FCGRT & B2M Heterodimer Protein, Rat, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 44 kDa and the accession number is P13599&P07151.
Species
Rat
Expression System
HEK293 Cells
TagC-His
Accession NumberP13599&P07151
ConstructionA DNA sequence encoding the extracellular domain (Met 1-Ser 298) of rat FCGRT (P13599) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the rat B2M (P07151) (Met 1-Met 119) constructed the plasmid 2. The two plasmids were co-expressed and the FCGRT/B2M heterodimer was purified. Predicted N terminal: Ala 23 & Ile 21
Protein Purity
> 95 % as determined by SDS-PAGE
FCGRT & B2M Heterodimer Protein, Rat, Recombinant (His)
Endotoxin< 1.0 EU/μg of the protein as determined by the LAL method.
FormulationLyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
ReconstitutionReconstituted with sterile deionized water to 0.25 mg/mL. Reconstitution conditions may vary depending on the lot.
SynonymsFc fragment of IgG, receptor, transporter, α, Fc fragment of IgG, receptor, transporter, alpha
Chemical Properties
Molecular Weight44 kDa (predicted); 35 & 12 kDa (reducing conditions)
Storage & Solubility Information
ShippingIn general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
StorageIt is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

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Related Tags: FCGRT & B2M Heterodimer Protein, Rat, Recombinant (His) chemical structure | FCGRT & B2M Heterodimer Protein, Rat, Recombinant (His) molecular weight