CRISPR-Cas9 Protein, Streptococcus pyogenes, Recombinant (His) is expressed in Baculovirus-Insect cells.
Pack Size | Availability | Price/USD | Quantity |
---|---|---|---|
200 μg | In stock | $ 386.00 |
Biological Information | 1 μg = 5 U1 U is defined as cutting 1 ug 1 KB dsDNA in 30 min at 37℃ |
Description | CRISPR-Cas9 Protein, Streptococcus pyogenes, Recombinant (His) is expressed in Baculovirus-Insect cells. |
Species | Streptococcus pyogenes |
Expression System | Baculovirus-Insect Cells |
Tag | His |
Accession Number | Q99ZW2 |
Synonyms | SpyCas9 |
Construction | A DNA sequence encoding the s. pyogenes serotype M1 cas9 (WP_010922251.1) (Met1-Asp1368) was expressed with a nuclear localization sequence (NLS) at the N-terminus, two NLSs and a polyhistidine tag at the C-terminus. |
Protein Purity |
≥ 90 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.
|
Molecular Weight | Approxiamtely 163 kDa |
Endotoxin | < 1.0 EU per μg protein as determined by the LAL method. |
Formulation | Supplied from sterile 20 mM Tris, 300 mM NaCl, 50 % glycerol, pH 8.0, 0. 5 mM PMSF, 0. 1 mM EDTA, 0. 5 mM TCEP. Please contact us for any concerns or special requirements. Please refer to the specific buffer information in the hard copy of CoA. |
Reconstitution | A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. |
Stability & Storage |
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Shipping |
Solution. It is shipped out with blue ice. |
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Please read the User Guide of Recombinant Proteins for more specific information.
CRISPR-Cas9 Protein, Streptococcus pyogenes, Recombinant (His) SpyCas9 recombinant recombinant-proteins proteins protein