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CRISPR-Cas9 Protein, Streptococcus pyogenes, Recombinant (His)

Catalog No. TMPY-05346
Synonyms: SpyCas9

CRISPR-Cas9 Protein, Streptococcus pyogenes, Recombinant (His) is expressed in Baculovirus-Insect cells.

All products from TargetMol are for Research Use Only. Not for Human or Veterinary or Therapeutic Use.
CRISPR-Cas9 Protein, Streptococcus pyogenes, Recombinant (His)
Pack Size Availability Price/USD Quantity
200 μg In stock $ 386.00
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Biological Description
Technical Params
Product Properties
Biological Information 1 μg = 5 U1 U is defined as cutting 1 ug 1 KB dsDNA in 30 min at 37℃
Description CRISPR-Cas9 Protein, Streptococcus pyogenes, Recombinant (His) is expressed in Baculovirus-Insect cells.
Species Streptococcus pyogenes
Expression System Baculovirus-Insect Cells
Tag His
Accession Number Q99ZW2
Synonyms SpyCas9
Construction A DNA sequence encoding the s. pyogenes serotype M1 cas9 (WP_010922251.1) (Met1-Asp1368) was expressed with a nuclear localization sequence (NLS) at the N-terminus, two NLSs and a polyhistidine tag at the C-terminus.
Protein Purity ≥ 90 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.

Molecular Weight Approxiamtely 163 kDa
Endotoxin < 1.0 EU per μg protein as determined by the LAL method.
Formulation Supplied from sterile 20 mM Tris, 300 mM NaCl, 50 % glycerol, pH 8.0, 0. 5 mM PMSF, 0. 1 mM EDTA, 0. 5 mM TCEP. Please contact us for any concerns or special requirements. Please refer to the specific buffer information in the hard copy of CoA.
Reconstitution A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Stability & Storage

Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Shipping

Solution. It is shipped out with blue ice.

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Tech Support

Please read the User Guide of Recombinant Proteins for more specific information.

Keywords

CRISPR-Cas9 Protein, Streptococcus pyogenes, Recombinant (His) SpyCas9 recombinant recombinant-proteins proteins protein

 

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