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CD16b Protein, Human, Recombinant (NA2, HEK293, His)
(Synonyms: FCRIIIb, FCRIII, FCR-10, FCGR3, FCG3, Fc γ RIIIb, Fc gamma RIIIb, Fc fragment of IgG receptor IIIb, CD16b, CD16) Copy Product InfoSynonyms: FCRIIIb, FCRIII, FCR-10, FCGR3, FCG3, Fc γ RIIIb, Fc gamma RIIIb, Fc fragment of IgG receptor IIIb, CD16b, CD16
Catalog No. TMPY-00701 Copy Product Info
CD16b Protein, Human, Recombinant (NA2, His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 22.2 kDa and the accession number is O75015.2.
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 5 µg | $48 | - | In Stock | |
| 10 µg | $75 | - | In Stock | |
| 20 µg | $118 | - | In Stock | |
| 50 µg | $229 | - | In Stock | |
| 100 µg | $386 | - | In Stock | |
| 200 µg | $682 | 7-10 days | 7-10 days | |
| 500 µg | $1,430 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Purity:SDS-PAGE: 97.2%; SEC-HPLC: 99.4%
Appearance:Lyophilized powder
Color:White
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Product Information
Bioactivity
Chemical Properties
Storage & Solubility Information
| Bioactivity | 1.Loaded Human FcγRIIIB / CD16b (NA2) recombinant protein on His1K Biosensor, can bind Bevacizumab (IgG1) with an affinity constant of 8.3 μM as determined in a BLI assay (Sartorius Octet Red384) (QC tested). 2.Captured Human FcγRIIIB / CD16b (NA2) recombinant protein on Anti-His Chip can bind IgG1 with an affinity constant of 2.6 μM as determined in an SPR assay (Biacore T200) (Routinely tested). 3.Captured IgG1 on proteinA Chip can bind Human FcγRIIIB/CD16b (NA2) recombinant protein with an affinity constant of 0.22 μM as determined in an SPR assay (Biacore T200) (Routinely tested). 4.The purity of Human CD16b/FCGR3B Protein (His Tag, NA2 allotype) is more than 95% and the molecular weight of this protein is around 33-43 kDa verified by SEC-MALS. |
| Description | CD16b Protein, Human, Recombinant (NA2, His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 22.2 kDa and the accession number is O75015.2. |
| Species | Human |
| Expression System | HEK293 Cells |
| Tag | C-His |
| Accession Number | O75015-2 |
| Construction | A DNA sequence encoding the human CD16b (O75015.2) (Met 1-Ser 200) was fused with a polyhistidine tag at the C-terminus. Predicted N terminal: Gly 17 |
| Protein Purity | > 95 % as determined by SDS-PAGE. > 90 % as determined by SEC-HPLC.  , |
| Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
| Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
| Reconstitution | Reconstituted with sterile deionized water to 0.25 mg/mL. Reconstitution conditions may vary depending on the lot. |
| Synonyms | FCRIIIb, FCRIII, FCR-10, FCGR3, FCG3, Fc γ RIIIb, Fc gamma RIIIb, Fc fragment of IgG receptor IIIb, CD16b, CD16 |
| Molecular Weight | 22.2 kDa (predicted); 46 kDa (reducing condition, due to glycosylation) |
| Shipping | In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice. |
| Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
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Related Tags: CD16b Protein, Human, Recombinant (NA2, HEK293, His) chemical structure | CD16b Protein, Human, Recombinant (NA2, HEK293, His) molecular weight
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