CD16b Protein, Human, Recombinant (NA2, His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 22.2 kDa and the accession number is O75015.2.
Pack Size | Availability | Price/USD | Quantity |
---|---|---|---|
100 μg | In stock | $ 386.00 | |
200 μg | 5 days | $ 682.00 | |
500 μg | 5 days | $ 1,440.00 |
Biological Information |
1.Loaded Human FcγRIIIB / CD16b (NA2) recombinant protein on His1K Biosensor, can bind Bevacizumab (IgG1) with an affinity constant of 8.3 μM as determined in a BLI assay (Sartorius Octet Red384) (QC tested). 2.Captured Human FcγRIIIB / CD16b (NA2) recombinant protein on Anti-His Chip can bind IgG1 with an affinity constant of 2.6 μM as determined in an SPR assay (Biacore T200) (Routinely tested). 3.Captured IgG1 on proteinA Chip can bind Human FcγRIIIB/CD16b (NA2) recombinant protein with an affinity constant of 0.22 μM as determined in an SPR assay (Biacore T200) (Routinely tested). 4.The purity of Human CD16b/FCGR3B Protein (His Tag, NA2 allotype) is more than 95% and the molecular weight of this protein is around 33-43 kDa verified by SEC-MALS.
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Description | CD16b Protein, Human, Recombinant (NA2, His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 22.2 kDa and the accession number is O75015.2. |
Species | Human |
Expression System | HEK293 Cells |
Tag | C-His |
Accession Number | O75015.2 |
Synonyms | Fc gamma RIIIb, FCR-10, FCRIIIb, Fc γ RIIIb, CD16b, CD16, FCG3, FCRIII, Fc fragment of IgG receptor IIIb, FCGR3 |
Construction | The Human CD16b (O75015.2) (Met 1-Ser 200) was fused with a polyhistidine tag at the C-terminus. |
Protein Purity |
> 95 % as determined by SDS-PAGE. > 90 % as determined by SEC-HPLC.
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Molecular Weight | 22.2 kDa (predicted) |
Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
Reconstitution | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
Stability & Storage |
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
Shipping |
In general, Lyophilized powders are shipping with blue ice. |
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Please read the User Guide of Recombinant Proteins for more specific information.
CD16b Protein, Human, Recombinant (NA2, His) FCGR-3 Fc gamma RIIIb FCR-10 FCR 10 FCRIIIb FCG 3 Fc γ RIIIb FCGR 3 CD16b FCG-3 CD16 FCG3 Fc gamma RIIIb FCR10 FCRIII CD 16 CD-16 Fc fragment of IgG receptor IIIb FCGR3 recombinant recombinant-proteins proteins protein