CD16a Protein, Human, Recombinant (176F, His)

Name: CD16a Protein, Human, Recombinant (176F, His)
Brand: TargetMol
SKU: TMPY-00763
Rating: 4.5 (1 reviews)

(Synonyms: IMD20, IGFR3, FCRIIIA, FCRIII, FCR-10, FCGRIII, FCGR3, FCG3, Fc γ RIIIa, Fc gamma RIIIa, Fc fragment of IgG, low affinity IIIa, receptor (CD16a), CD16A, CD16) Copy Product Info

Synonyms: IMD20, IGFR3, FCRIIIA, FCRIII, FCR-10, FCGRIII, FCGR3, FCG3, Fc γ RIIIa, Fc gamma RIIIa, Fc fragment of IgG, low affinity IIIa, receptor (CD16a), CD16A, CD16

Catalog No. TMPY-00763 Copy Product Info

CD16a Protein, Human, Recombinant (176F, His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 23.3 kDa and the accession number is P08637-1.

Pack Size	Price	USA Stock	Global Stock
5 µg	$68	7-10 days	7-10 days
10 µg	$109	7-10 days	7-10 days
20 µg	$172	7-10 days	7-10 days
50 µg	$343	7-10 days	7-10 days
100 µg	$682	7-10 days	7-10 days
200 µg	$983	7-10 days	7-10 days
500 µg	$1,900	7-10 days	7-10 days
1 mg	$3,680	7-10 days	7-10 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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User Guide of Recombinant Proteins

Product Introduction

Bioactivity

Bioactivity	Loaded Human IgG1 Fc on Protein-A Biosensor, can bind Human CD16a (F176)-His with an affinity constant of 1.35 uM as determined in BLI assay. (Regularly tested) Loaded Human CD16a (F176)-His on HIS1K Biosensor, can bind Anti-Human HER2 mAb with an affinity constant of 3.4 uM as determined in BLI assay. Loaded Human CD16a (F176)-His on HIS1K Biosensor, can bind Anti-Human CD16 mAb with an affinity constant of 0.07 nM as determined in BLI assay.
Description	CD16a Protein, Human, Recombinant (176F, His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 23.3 kDa and the accession number is P08637-1.
Species	Human
Expression System	HEK293 Cells
Tag	C-6xHis
Accession Number	P08637
Construction	Gly17-Gln208
Protein Purity	> 95 % as determined by SDS-PAGE. > 90 % as determined by SEC-HPLC
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Reconstitution	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Synonyms	IMD20, IGFR3, FCRIIIA, FCRIII, FCR-10, FCGRIII, FCGR3, FCG3, Fc γ RIIIa, Fc gamma RIIIa, Fc fragment of IgG, low affinity IIIa, receptor (CD16a), CD16A, CD16
Research Background	The Fc receptor with low affinity for IgG (FCGR3, or CD16) is encoded by 2 nearly identical genes, FCGR3A and FCGR3B, resulting in tissue-specific expression of alternative membrane-anchored isoforms. FCGR3A, it is also known as CD16a, encodes a transmembrane protein expressed on activated monocytes/macrophages, natural killer (NK) cells, and a subset of T cells. CD16a / FCGR3A is a receptor expressed on NK cells that facilitates antibody dependent cellular cytotoxicity (ADCC) by binding to the Fc portion of various antibodies. CD16a / FCGR3A also has a broader function. CD16a / FCGR3A is directly involved in the lysis of some virus-infected cells and tumor cells by NK cells, independent of antibody binding. Cross-linking of CD16a / FCGR3A on NK cells resulted in increased intracellular Ca2+ levels and a cascade of biochemical events similar to those activated by the T cell receptor. CD16a / FCGR3A on human NK cells is a lysis receptor that mediates the direct killing of some virus infected and tumor cells, independent of antibody ligation.

Chemical Properties

Molecular Weight

22.7 kDa (predicted); 35-50 kDa (reducing condition, due to glycosylation)

Storage & Solubility Information

Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.