1.Loaded Human FcγRIIIA / CD16a (F176) recombinant protein on His1K Biosensor, can bind Bevacizumab (IgG1) with an affinity constant of 1.9 μM as determined in a BLI assay. 2.Captured Human FcγRIIIA / CD16a (F176) recombinant protein  on Anti-His Chip can bind Human IgG Fc  with an affinity constant of 7.1 μM as determined in an SPR assay (Biacore T200). 3.Captured Bevacizumab (IgG1) on proA Chip can bind Human FcγRIIIA / CD16a (F176) recombinant protein with an affinity constant of 2.67 µM as determined in an SPR assay. 4. The purity of Human CD16a / FCGR3A Protein (176 Phe, His Tag) is more than 95% and the molecular weight of this protein is around 36-48 kDa verified by SEC-MALS.

CD16a Protein, Human, Recombinant (176F, His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 23.3 kDa and the accession number is P08637-1.

Human

HEK293 Cells

IMD20,IGFR3,FCRIIIA,FCRIII,FCR-10,FCGRIII,FCGR3,FCG3,Fc γ RIIIa,Fc gamma RIIIa,Fc fragment of IgG, low affinity IIIa, receptor (CD16a),CD16A,CD16

A DNA sequence encoding the extracellular domain (Met 1-Gln 208) of human CD16a (P08637-1) precursor was expressed with a C-terminal polyhistidine tag. Predicted N terminal: Gly 17

> 95 % as determined by SDS-PAGE. > 90 % as determined by SEC-HPLC

A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.

It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

The Fc receptor with low affinity for IgG (FCGR3, or CD16) is encoded by 2 nearly identical genes, FCGR3A and FCGR3B, resulting in tissue-specific expression of alternative membrane-anchored isoforms. FCGR3A, it is also known as CD16a, encodes a transmembrane protein expressed on activated monocytes/macrophages, natural killer (NK) cells, and a subset of T cells. CD16a / FCGR3A is a receptor expressed on NK cells that facilitates antibody dependent cellular cytotoxicity (ADCC) by binding to the Fc portion of various antibodies. CD16a / FCGR3A also has a broader function. CD16a / FCGR3A is directly involved in the lysis of some virus-infected cells and tumor cells by NK cells, independent of antibody binding. Cross-linking of CD16a / FCGR3A on NK cells resulted in increased intracellular Ca2+ levels and a cascade of biochemical events similar to those activated by the T cell receptor. CD16a / FCGR3A on human NK cells is a lysis receptor that mediates the direct killing of some virus infected and tumor cells, independent of antibody ligation.