CD146/MCAM Protein, Human, Recombinant (His), Biotinylated

Catalog No. TMPY-06064

CD146/MCAM Protein, Human, Recombinant (His), Biotinylated is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 61.3 kDa and the accession number is A0A024R3I5.

CD146/MCAM Protein, Human, Recombinant (His), Biotinylated

Catalog No. TMPY-06064

CD146/MCAM Protein, Human, Recombinant (His), Biotinylated is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 61.3 kDa and the accession number is A0A024R3I5.

Pack Size	Price	USA Warehouse	Global Warehouse
5 μg	$172	7-10 days	7-10 days
10 μg	$298	7-10 days	7-10 days
20 μg	$498	7-10 days	7-10 days
50 μg	$823	7-10 days	7-10 days
100 μg	$1,380	7-10 days	7-10 days

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In Stock Estimated shipping dateUSA Warehouse [1-2 days] Global Warehouse [5-7 days]

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Product Information

Biological Activity	Activity testing is in progress. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
Description	CD146/MCAM Protein, Human, Recombinant (His), Biotinylated is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 61.3 kDa and the accession number is A0A024R3I5.
Species	Human
Expression System	HEK293 Cells
Tag	C-His
Accession Number	P43121
Synonyms	MUC18,melanoma cell adhesion molecule,CD146
Construction	A DNA sequence encoding the extracellular domain of human CD146 precursor (NP_006491.2) (Met1-Gly559) was expressed with a C-terminal polyhistidine tag. The purified protein was biotinylated in vitro. Predicted N terminal: Val 24
Protein Purity	> 95 % as determined by SDS-PAGE.
Molecular Weight	61.3 kDa (predicted); 88.9 kDa (reducing condition, due to glycosylation)
Endotoxin	< 1.0 EU/μg protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	The CD146 antigen, also known as melanoma cell adhesion molecule (MCAM) and MUC18, is an integral membrane glycoprotein belonging to the immunoglobulin superfamily. CD146 contains the characteristic immunoglobulin-like domains (V-V-C2-C2-C2), a transmembrane region, and a short cytoplasmic tail. The CD146 expression is detected in endothelial cells in vascular tissue throughout the body and plays a role in cell adhesion, as well as in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. As a Ca2+-independent cell adhesion molecule involved in heterophilic cell to cell interactions and a surface receptor, CD146 triggers tyrosine phosphorylation of FYN and PTK2 and subsequently induced signal transduction, proteolysis, or immune recognition. This protein is also expressed predominantly on metastatic lesions and advanced primary tumors, and thus has been suggested to play an important role in tumor progression and the development of metastasis in certain human carcinomas.