ART4/CD297 Protein, Rat, Recombinant (His)

Name: ART4/CD297 Protein, Rat, Recombinant (His)
Brand: TargetMol
SKU: TMPY-03312
Rating: 4.5 (1 reviews)

(Synonyms: ADP-ribosyltransferase 4 (Dombrock blood group)) Copy Product Info

Synonyms: ADP-ribosyltransferase 4 (Dombrock blood group)

Catalog No. TMPY-03312 Copy Product Info

ART4/CD297 Protein, Rat, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 28.4 kDa and the accession number is F1MA10.

Pack Size	Price	USA Stock	Global Stock
5 µg	$79	7-10 days	7-10 days
10 µg	$129	7-10 days	7-10 days
20 µg	$208	7-10 days	7-10 days
50 µg	$419	7-10 days	7-10 days
100 µg	$812	7-10 days	7-10 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

Add to Cart

Add to Quotation

For research use only—not for human use. No sales to individuals. Use as intended only.

Questions

Resource Download

User Guide of Recombinant Proteins

Product Information

Bioactivity

Bioactivity	Activity testing is in progress. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
Description	ART4/CD297 Protein, Rat, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 28.4 kDa and the accession number is F1MA10.
Species	Rat
Expression System	HEK293 Cells
Tag	C-His
Accession Number	F1MA10
Construction	A DNA sequence encoding the rat ART4 (NP_001166980.1)(Met1-Lys269) was expressed with a polyhistidine tag at the C-terminus. Predicted N terminal: Gly 30
Protein Purity	> 95 % as determined by SDS-PAGE
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Synonyms	ADP-ribosyltransferase 4 (Dombrock blood group)
Research Background	ADP-ribosyltransferase 4 (Dombrock blood group), also known as Mono-ADP-ribosyltransferase 4(ART4), Dombrock blood group carrier molecule and CD297, is a protein that contains a mono-ADP-ribosylation (ART) motif. It is a member of the ADP-ribosyltransferase gene family but enzymatic activity has not been demonstrated experimentally. ADP-ribosyltransferase catalyzes the ADP-ribosylation of arginine residues in proteins. Mono-ADP-ribosylation is a posttranslational modification of proteins that is interfered with by a variety of bacterial toxins including cholera, pertussis, and heat-labile enterotoxins of E. coli. ART4 could be detected on HEL cells and erythrocytes by FACS analysis while it was absent from activated monocytes, despite the presence of ART4 mRNA in these cells. ART is also known as the carrier of the Dombrock blood group alloantigens (Do) which is glycosylphosphatidylinosotol-anchored to the erythrocyte membrane.

Chemical Properties

Molecular Weight

28.4 kDa (predicted); 36-41 kDa (reducing condition, due to glycosylation)

Storage & Solubility Information

Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.