Argonaute-2 Protein, Mouse, Recombinant (His)

Catalog No. TMPY-02130

Synonyms: mKIAA4215, 1110029L17Rik, 2310051F07Rik, Ago2, argonaute RISC catalytic component 2, ENSMUSG00000072493, KIAA4215, Gerp95, AI225898, AL022874, Gm10365, Eif2c2, AW546247

Argonaute 2 (AGO2), also known as Eukaryotic translation initiation factor 2C2 (EIF2C2), belongs to the Argonaute family, AGO subfamily, which is a component of the RNA-induced silencing complex (RISC) and mediates small interfering RNA (siRNA)-directed mRNA cleavage and microRNA translational suppression. AGO2 protein is the catalytic engine of mammalian RNAi. It contains a PIWI domain that is structurally related to RNases H and possibly shares with them a two-metal-ion catalysis mechanism. Human AGO2 was unable to cleave preformed RNA duplexes and exhibited weaker binding affinity for RNA duplexes compared with the single strand RNA. The enzyme exhibited greater RNase H activity in the presence of Mn2+ compared with Mg2+. Human AGO2 exhibited weaker binding affinities and reduced cleavage activities for antisense RNAs with either a 5'-terminal hydroxyl or abasic nucleotide. In mouse hematopoiesis, AGO2 controls early development of lymphoid and erythroid cells. AGO2 is a highly specialized member of the Argonaute family with an essential nonredundant Slicer-independent function within the mammalian miRNA pathway. AGO2 regulates dFMR1 expression, and the relationship between dFMR1 and AGO2 was defined by their physical interaction and co-regulation of downstream targets. AGO2 and dFMR1 are also connected through a regulatory relationship. AGO2 is a regulator of dFMR1 expression and have clarified an important developmental role for AGO2 in the nervous system and germ line that requires dFMR1 function. In addition, AGO2 is regulated at both the transcriptional and posttranslational level, and also implicate AGO2 and enhanced micro-RNA activity in the tumorigenic progression of breast cancer cell lines.

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Argonaute-2 Protein, Mouse, Recombinant (His)

Pack Size	Availability	Price/USD
20 μg	In stock	$ 162.00
100 μg	5 days	$ 559.00
200 μg	5 days	$ 952.00
500 μg	5 days	$ 1,930.00

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Biological Description

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References and Literature

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Description

Species	Mouse
Expression System	Baculovirus-Insect Cells
Tag	His
Accession Number	Q8CJG0
Synonyms	mKIAA4215, 1110029L17Rik, 2310051F07Rik, Ago2, argonaute RISC catalytic component 2, ENSMUSG00000072493, KIAA4215, Gerp95, AI225898, AL022874, Gm10365, Eif2c2, AW546247
Construction	A DNA sequence encoding the mouse AGO2 (Q8CJG0) (Met 1-Ala 860) was fused with a polyhistidine tag at the N-terminus.
Protein Purity	> 80 % as determined by SDS-PAGE
Molecular Weight	Approxiamtely 99 kDa

Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method.
Formulation	Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.4, 10% glyPlease contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0. 01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Reconstitution	A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Stability & Storage	Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Shipping	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Research Background	Argonaute 2 (AGO2), also known as Eukaryotic translation initiation factor 2C2 (EIF2C2), belongs to the Argonaute family, AGO subfamily, which is a component of the RNA-induced silencing complex (RISC) and mediates small interfering RNA (siRNA)-directed mRNA cleavage and microRNA translational suppression. AGO2 protein is the catalytic engine of mammalian RNAi. It contains a PIWI domain that is structurally related to RNases H and possibly shares with them a two-metal-ion catalysis mechanism. Human AGO2 was unable to cleave preformed RNA duplexes and exhibited weaker binding affinity for RNA duplexes compared with the single strand RNA. The enzyme exhibited greater RNase H activity in the presence of Mn2+ compared with Mg2+. Human AGO2 exhibited weaker binding affinities and reduced cleavage activities for antisense RNAs with either a 5'-terminal hydroxyl or abasic nucleotide. In mouse hematopoiesis, AGO2 controls early development of lymphoid and erythroid cells. AGO2 is a highly specialized member of the Argonaute family with an essential nonredundant Slicer-independent function within the mammalian miRNA pathway. AGO2 regulates dFMR1 expression, and the relationship between dFMR1 and AGO2 was defined by their physical interaction and co-regulation of downstream targets. AGO2 and dFMR1 are also connected through a regulatory relationship. AGO2 is a regulator of dFMR1 expression and have clarified an important developmental role for AGO2 in the nervous system and germ line that requires dFMR1 function. In addition, AGO2 is regulated at both the transcriptional and posttranslational level, and also implicate AGO2 and enhanced micro-RNA activity in the tumorigenic progression of breast cancer cell lines.

References and Literature

1. O'Carroll D,et al.(2007) A Slicer-independent role for Argonaute 2 in hematopoiesis and the microRNA pathway. Genes Dev. 21(16): 1999-2004. 2. Pepper AS, et al. (2009) Argonaute2 suppresses Drosophila fragile X expression preventing neurogenesis and oogenesis defects. PLoS One. 4(10): e7618.Lima WF,et al.(2009) Binding and cleavage specificities of human Argonaute2. J Biol Chem. 284(38): 26017-28. 3. Adams BD,et al.(2009) Argonaute-2 expression is regulated by epidermal growth factor receptor and mitogen-activated protein kinase signaling and correlates with a transformed phenotype in breast cancer cells. Endocrinology. 150(1): 14-23. 4. Salvatore V,et al.(2010) Bacterial expression of mouse argonaute 2 for functional and mutational studies. Int J Mol Sci. 11(2): 745-53. 5. Wilson JA,et al.(2011) Human Ago2 is required for efficient microRNA 122 regulation of hepatitis C virus RNA accumulation and translation. J Virol. 85(5): 2342-50.

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Keywords

Argonaute-2 Protein, Mouse, Recombinant (His) AW-546247 mKIAA4215 1110029L17Rik KIAA-4215 mKIAA 4215 2310051F07Rik Ago2 argonaute RISC catalytic component 2 mKIAA-4215 AL-022874 ENSMUSG00000072493 KIAA4215 Gerp95 AI225898 AW 546247 AL022874 Gm10365 AI-225898 Eif2c2 AW546247 ENSMUSG-00000072493 AI 225898 ENSMUSG 00000072493 AL 022874 KIAA 4215 recombinant recombinant-proteins proteins protein