LILRB4/CD85k/ILT3 Domain 1 Protein, Human, Recombinant (His & Avi), Biotinylated

LILRB4,also known as CD85k and LIR-5, ILT-3, is an approximately 60 kDa transmembrane glycoprotein that negatively regulates immune cell activation. Mature human ILT-3 consists of a 238 amino acid (aa) extracellular domain with two Ig-like domains, a 21 aa transmembrane segment, and a 168 aa cytoplasmic domain with 3 immunoreceptor tyrosine-based inhibitory motifs (ITIM).LILRB4 is receptor for class I MHC antigens. Recognizes a broad spectrum of HLA-A, HLA-B, HLA-C and HLA-G alleles.

Pack Size	Availability	Price/USD
100 μg	5 days	$ 1,050.00
500 μg	5 days	$ 4,200.00
1 mg	5 days	$ 6,990.00

Pack Size

Availability

Price/USD

Quantity

100 μg

5 days

$ 1,050.00

500 μg

5 days

$ 4,200.00

1 mg

5 days

$ 6,990.00

Description

Species	Human
Expression System	HEK293
Tag	C-His-Avi
Accession Number	AAH26309.1
Synonyms	CD85K, HM18, ILT-3, ILT3, LILRB4, LIR5
Construction	Recombinant Biotinylated Human LILRB4/CD85k/ILT3 Domain 1 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Gln22-Thr118.[Accession \|AAH26309.1]
Protein Purity	> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Molecular Weight	The protein has a predicted MW of 14.26 kDa. Due to glycosylation, the protein migrates to 14.5-20 kDa based on Tris-Bis PAGE result.

Endotoxin	Less than 1EU per μg by the LAL method.
Formulation	Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability & Storage	-20 to -80°C for 12 months as supplied from date of receipt. -20 to -80°C for 3-6 months in unopened state after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Shipping	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Research Background	LILRB4,also known as CD85k and LIR-5, ILT-3, is an approximately 60 kDa transmembrane glycoprotein that negatively regulates immune cell activation. Mature human ILT-3 consists of a 238 amino acid (aa) extracellular domain with two Ig-like domains, a 21 aa transmembrane segment, and a 168 aa cytoplasmic domain with 3 immunoreceptor tyrosine-based inhibitory motifs (ITIM).LILRB4 is receptor for class I MHC antigens. Recognizes a broad spectrum of HLA-A, HLA-B, HLA-C and HLA-G alleles.