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USP25/28 inhibitor AZ1

Name: USP25/28 inhibitor AZ1
Brand: TargetMol
SKU: T7685
Price: 30 USD
Availability: InStock
Rating: 5 (10 reviews)

🥰Excellent

Catalog No. T7685Cas No. 2165322-94-9

Alias AZ1

USP25/28 inhibitor AZ1 is a selective, orally active and non-competitive dual ubiquitin-specific protease USP25/28 inhibitor with IC50 of 0.7 μM and 0.6 μM, respectively, and exhibits anti-inflammatory and anti-tumor effects.

USP25/28 inhibitor AZ1

🥰Excellent

Purity: 99.79%

Catalog No. T7685Alias AZ1Cas No. 2165322-94-9

Pack Size	Price	USA Warehouse	Global Warehouse
1 mg	$30	In Stock	In Stock
5 mg	$64	In Stock	In Stock
10 mg	$98	In Stock	In Stock
25 mg	$222	In Stock	In Stock
50 mg	$338	In Stock	In Stock
100 mg	$497	In Stock	In Stock
500 mg	$1,090	-	In Stock
1 mL x 10 mM (in DMSO)	$71	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Purity:99.79%

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COA HPLC HNMR

Product Introduction

Bioactivity

Description	USP25/28 inhibitor AZ1 is a selective, orally active and non-competitive dual ubiquitin-specific protease USP25/28 inhibitor with IC50 of 0.7 μM and 0.6 μM, respectively, and exhibits anti-inflammatory and anti-tumor effects.
Targets&IC50	USP28:0.6 nM, USP25:0.7 nM
In vitro	METHODS: HCT116 cells were treated with USP25/28 inhibitor AZ1 (20, 40, 60, 80, 100 μM) and samples were collected 3 hours later for Western blot analysis of USP28, c-Myc and PARP. RESULTS Western blot analysis showed that the total protein level of c-Myc decreased rapidly and concentration-dependently after treatment with USP25/28 inhibitor AZ1. [1]
In vivo	Method: USP25/28 inhibitor AZ1 (AZ1) (20, 40mg/kg, gavage) was given to WT Model mice were used to study the effect of USP25/28 inhibitor AZ1 (AZ1) on DSS-induced colitis. RESULTS USP25/28 inhibitor AZ1 (AZ1) treatment significantly reduced the number of colon tumors; the expression of wnt-related genes and pSTAT3 levels in tumors in the USP25/28 inhibitor AZ1 (AZ1) treatment group were reduced, and the SOCS3 level was increased; USP25/28 inhibitor Inflammation and bacterial replication were impaired in the colon of mice gavaged with AZ1 (AZ1), the expression of pro-inflammatory cytokines and antimicrobial peptides was enhanced, p-p65 and p-p38 levels were increased, and TRAF3 levels were decreased. [2]
Synonyms	AZ1

Chemical Properties

Molecular Weight	422.21
Formula	C₁₇H₁₆BrF₄NO₂
Cas No.	2165322-94-9
Smiles	OCCNCc1cc(Br)ccc1OCc1ccc(F)c(c1)C(F)(F)F
Relative Density.	1.488 g/cm3 (Predicted)
Color	White
Appearance	solid

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 250 mg/mL (592.12 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 5 mg/mL (11.84 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.3685 mL	11.8424 mL	23.6849 mL	118.4245 mL
5 mM	0.4737 mL	2.3685 mL	4.7370 mL	23.6849 mL
10 mM	0.2368 mL	1.1842 mL	2.3685 mL	11.8424 mL
20 mM	0.1184 mL	0.5921 mL	1.1842 mL	5.9212 mL
50 mM	0.0474 mL	0.2368 mL	0.4737 mL	2.3685 mL
100 mM	0.0237 mL	0.1184 mL	0.2368 mL	1.1842 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:

For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . TargetMol | Animal experiments

A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent

DMSO+30% PEG300+5% Tween 80+60% Saline/PBS/ddH₂O. So your working solution concentration is 2 mg/mL。

Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSO TargetMol | reagent

(mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.

Preparation method for in vivo formula: Take 50 μL DMSO TargetMol | reagent

main solution, add 300 μLPEG300 TargetMol | reagent

mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLSaline/PBS/ddH₂O TargetMol | reagent

mix well and clarify

For Reference Only. Please develop an appropriate dissolution method based on your laboratory animals and route of administration.

All types of co-solvents required for the protocol, such asDMSO, PEG300/ PEG400, Tween 80, SBE-β-CD, corn oil are available for purchase on the TargetMol website with a simple click.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc