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Tetrahydrobiopterin

Name: Tetrahydrobiopterin
Brand: TargetMol
SKU: T17056
Rating: 4.5 (1 reviews)

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Catalog No. T17056Cas No. 17528-72-2

Alias Sapropterin, BH4, (Rac)-Sapropterin

Tetrahydrobiopterin (BH4) is a cofactor for aromatic amino acid hydroxylases and an essential cofactor for nitric oxide synthase (NOS), which is used in the study of endothelial dysfunction such as hypertension, hypercholesterolemia, and diabetes.

Tetrahydrobiopterin

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Purity: 99.82%

Catalog No. T17056Alias Sapropterin, BH4, (Rac)-SapropterinCas No. 17528-72-2

Pack Size	Price	Availability
1 mg	$40	7-10 days
5 mg	$87	7-10 days
10 mg	$125	7-10 days
25 mg	$198	7-10 days
50 mg	$297	7-10 days
100 mg	$453	7-10 days
200 mg	$694	7-10 days
1 mL x 10 mM (in DMSO)	$98	In Stock

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Purity:99.82%

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COA HPLC HNMR

Product Introduction

Bioactivity

Description	Tetrahydrobiopterin (BH4) is a cofactor for aromatic amino acid hydroxylases and an essential cofactor for nitric oxide synthase (NOS), which is used in the study of endothelial dysfunction such as hypertension, hypercholesterolemia, and diabetes.
In vitro	Microglia cultures under hyperoxic conditions were supplemented with or without Tetrahydrobiopterin (100 μM). Exposure of microglia to hyperoxia-induced oxidative stress for 24 h showed a significant increase in TSP-1 mRNA expression and protein compared with normoxia (21% O2). Tetrahydrobiopterin supplementation significantly prevents hyperoxia-induced microglial activation and prevents microvascular damage in choroidal explants by reducing Iba-1 and TSP-1 expression.[1]
In vivo	To assess the levels of tetrahydrobiopterin in the retina, three to five retinal pools were collected from WT and HPH-1 mice at postnatal ages 7, 14, and 22 years and evaluated by LC-MS/MS. LC-MS/MS analysis confirmed that the concentration level of tetrahydrobiopterin in the retinal tissue of HPH-1 mice was significantly reduced by approximately 90% compared with WT groups P7, P14, and P22, respectively.[1]
Synonyms	Sapropterin, BH4, (Rac)-Sapropterin

Chemical Properties

Molecular Weight	241.25
Formula	C₉H₁₅N₅O₃
Cas No.	17528-72-2
Smiles	CC(O)C(O)C1CNc2[nH]c(N)nc(=O)c2N1
Relative Density.	1.31g/cm3

Storage & Solubility Information

Storage

store at low temperature,keep away from direct sunlight | Powder: -20°C for 3 years | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 40 mg/mL (165.80 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 2 mg/mL (8.29 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	4.1451 mL	20.7254 mL	41.4508 mL	207.2539 mL
5 mM	0.8290 mL	4.1451 mL	8.2902 mL	41.4508 mL
10 mM	0.4145 mL	2.0725 mL	4.1451 mL	20.7254 mL
20 mM	0.2073 mL	1.0363 mL	2.0725 mL	10.3627 mL
50 mM	0.0829 mL	0.4145 mL	0.8290 mL	4.1451 mL
100 mM	0.0415 mL	0.2073 mL	0.4145 mL	2.0725 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:

For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . TargetMol | Animal experiments

A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent

DMSO+30% PEG300+5% Tween 80+60% Saline/PBS/ddH₂O. So your working solution concentration is 2 mg/mL。

Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSO TargetMol | reagent

(mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.

Preparation method for in vivo formula: Take 50 μL DMSO TargetMol | reagent

main solution, add 300 μLPEG300 TargetMol | reagent

mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLSaline/PBS/ddH₂O TargetMol | reagent

mix well and clarify

For Reference Only. Please develop an appropriate dissolution method based on your laboratory animals and route of administration.

All types of co-solvents required for the protocol, such asDMSO, PEG300/ PEG400, Tween 80, SBE-β-CD, corn oil are available for purchase on the TargetMol website with a simple click.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc