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Pyroxamide

Name: Pyroxamide
Brand: TargetMol
SKU: T7664
Price: 43 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T7664Cas No. 382180-17-8

Pyroxamide is an histone deacetylases (HDACs) inhibitor with antineoplastic properties ( HDAC1;IC50: 0.1-0.2 μM).

Pyroxamide

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Purity: 98.46%

Catalog No. T7664Cas No. 382180-17-8

Pyroxamide is an histone deacetylases (HDACs) inhibitor with antineoplastic properties ( HDAC1;IC50: 0.1-0.2 μM).

Pack Size	Price	USA Warehouse	Global Warehouse
5 mg	$43	In Stock	In Stock
10 mg	$75	In Stock	In Stock
25 mg	$162	In Stock	In Stock
50 mg	$279	In Stock	In Stock
100 mg	$492	In Stock	In Stock
1 mL x 10 mM (in DMSO)	$48	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

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Batch Information

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Purity:98.46%

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COA LCMS HNMR

Product Introduction

Bioactivity

Description	Pyroxamide is an histone deacetylases (HDACs) inhibitor with antineoplastic properties ( HDAC1;IC50: 0.1-0.2 μM).
Targets&IC50	HDAC1:0.1-0.2 μM
In vitro	The activity of pyroxamide as an inhibitor of HDACs.?Pyroxamide inhibited the enzymatic activity of affinity-purified HDAC1 at submicromolar concentrations ?.?The ID50 value for inhibition of HDAC1 activity by pyroxamide was ～100 nm.MEL cells incubated with pyroxamide (4 μm) for 4, 24, or 48 h showed accumulation of acetylated histones H2A, H2B, H3, and H4 .?Cells cultured without the agent had low basal levels of acetylated histones at the same time points[1]
In vivo	Pyroxamide may be a useful agent for the treatment of malignancy and that induction of p21/WAF1 in transformed cells by pyroxamide may contribute to the antitumor effects of this agent[1].
Cell Research	HDAC1 enzyme assay, ?A MEL cell line expressing the epitope Flag-tagged HDAC1 was generated.?HDAC1-Flag was affinity purified by immunoprecipitation using M2 anti-Flag antibody-coated agarose, followed by elution from the agarose using the Flag peptide.?[3H]acetate-labeled cellular histones were prepared from MEL cells and were used as a substrate for the HDAC activity assay.?Released [3H]acetic acid was quantified by scintillation counting.?For inhibition studies, the enzyme preparations were preincubated with pyroxamide (10 to 100,000 nm) for 30 min at 4°C.[1]

Chemical Properties

Molecular Weight	265.31
Formula	C₁₃H₁₉N₃O₃
Cas No.	382180-17-8
Smiles	ONC(=O)CCCCCCC(=O)Nc1cccnc1
Relative Density.	1.215 g/cm3

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 125 mg/mL (471.15 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 4 mg/mL (15.08 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	3.7692 mL	18.8459 mL	37.6918 mL	188.4588 mL
5 mM	0.7538 mL	3.7692 mL	7.5384 mL	37.6918 mL
10 mM	0.3769 mL	1.8846 mL	3.7692 mL	18.8459 mL
20 mM	0.1885 mL	0.9423 mL	1.8846 mL	9.4229 mL
50 mM	0.0754 mL	0.3769 mL	0.7538 mL	3.7692 mL
100 mM	0.0377 mL	0.1885 mL	0.3769 mL	1.8846 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:

For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . TargetMol | Animal experiments

A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent

DMSO+30% PEG300+5% Tween 80+60% Saline/PBS/ddH₂O. So your working solution concentration is 2 mg/mL。

Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSO TargetMol | reagent

(mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.

Preparation method for in vivo formula: Take 50 μL DMSO TargetMol | reagent

main solution, add 300 μLPEG300 TargetMol | reagent

mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLSaline/PBS/ddH₂O TargetMol | reagent

mix well and clarify

For Reference Only. Please develop an appropriate dissolution method based on your laboratory animals and route of administration.

All types of co-solvents required for the protocol, such asDMSO, PEG300/ PEG400, Tween 80, SBE-β-CD, corn oil are available for purchase on the TargetMol website with a simple click.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc