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Prostratin

Name: Prostratin
Brand: TargetMol
SKU: T21441
Rating: 4.5 (1 reviews)

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Catalog No. T21441Cas No. 60857-08-1

Alias 12-Deoxyphorbol-13-acetate

Prostratin (12-Deoxyphorbol-13-acetate) is an orally active protein kinase C (PKC) activator with a Ki value of 12.5 nM.As an NF-κB activator, Prostratin activates HIV gene expression in T cells.Prostratin inhibits the growth of acute myeloid leukemia (AML) cell lines and enhances cytarabine-induced AML cell differentiation. In addition, Prostratin induced KRAS phosphorylation and inhibited the growth of KRAS-mutated tumors.

Prostratin

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Catalog No. T21441Alias 12-Deoxyphorbol-13-acetateCas No. 60857-08-1

Pack Size	Price	USA Warehouse	Global Warehouse	Quantity
500 μg	$154	35 days	35 days
1 mg	$211	35 days	35 days

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Product Introduction

Bioactivity

Description	Prostratin (12-Deoxyphorbol-13-acetate) is an orally active protein kinase C (PKC) activator with a Ki value of 12.5 nM.As an NF-κB activator, Prostratin activates HIV gene expression in T cells.Prostratin inhibits the growth of acute myeloid leukemia (AML) cell lines and enhances cytarabine-induced AML cell differentiation. In addition, Prostratin induced KRAS phosphorylation and inhibited the growth of KRAS-mutated tumors.
Targets&IC50	PKC:12.5 nM (Ki)
In vitro	Methods: Prostratin (125, 250, 500, 1000 nM) was used to treat HL-60, NB4, and U937 cells to evaluate its effect on leukemia cell growth. Results: Prostratin showed similar concentration- and time-dependent growth inhibition on all three cell lines. [2]
Synonyms	12-Deoxyphorbol-13-acetate

Chemical Properties

Molecular Weight	390.47
Formula	C₂₂H₃₀O₆
Cas No.	60857-08-1
Smiles	O(C(C)=O)[C@@]12[C@@](C1(C)C)([C@]3([C@](O)([C@H](C)C2)[C@]4([C@](O)(CC(CO)=C3)C(=O)C(C)=C4)[H])[H])[H]
Relative Density.	1.31 g/cm3
Color	White
Appearance	Solid

Storage & Solubility Information

Storage

store at low temperature | store at 4°C | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 40 mg/mL (102.44 mM), Sonication is recommended.

H2O: < 1 mg/mL (insoluble)

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween-80+45% Saline: 1.5 mg/mL (3.84 mM), Sonication is recommeded.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.5610 mL	12.8051 mL	25.6102 mL	128.0508 mL
5 mM	0.5122 mL	2.5610 mL	5.1220 mL	25.6102 mL
10 mM	0.2561 mL	1.2805 mL	2.5610 mL	12.8051 mL
20 mM	0.1281 mL	0.6403 mL	1.2805 mL	6.4025 mL
50 mM	0.0512 mL	0.2561 mL	0.5122 mL	2.5610 mL
100 mM	0.0256 mL	0.1281 mL	0.2561 mL	1.2805 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:

For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . TargetMol | Animal experiments

A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent

DMSO+30% PEG300+5% Tween 80+60% Saline/PBS/ddH₂O. So your working solution concentration is 2 mg/mL。

Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSO TargetMol | reagent

(mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.

Preparation method for in vivo formula: Take 50 μL DMSO TargetMol | reagent

main solution, add 300 μLPEG300 TargetMol | reagent

mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLSaline/PBS/ddH₂O TargetMol | reagent

mix well and clarify

For Reference Only. Please develop an appropriate dissolution method based on your laboratory animals and route of administration.

All types of co-solvents required for the protocol, such asDMSO, PEG300/ PEG400, Tween 80, SBE-β-CD, corn oil are available for purchase on the TargetMol website with a simple click.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc