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Pifithrin-β hydrobromide

Name: Pifithrin-β hydrobromide
Brand: TargetMol
SKU: T3637
Price: 34 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T3637Cas No. 511296-88-1

Alias Pifithrin-β, PFT-β, PFT β (hydrobromide), Cyclic Pifithrin-α hydrobromide, Cyclic PFT-α

Pifithrin-β hydrobromide (Cyclic PFT-α) is an inhibitor of p53; reversibly blocks p53-dependent transcriptional activation and apoptosis. Protects against neuronal death in models of stroke and neurodegenerative disorders. Active in vivo; protects mice from the side-effects of Y therapy associated with p53 induction. Supresses self-renewal of embryonic stem cells. Also aryl hydrocarbon receptor (AHR) agonist, causes upregulation of AHR target gene CYP1A1 (EC50 = 1.1 μM).

Pifithrin-β hydrobromide

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Purity: 99.74%

Catalog No. T3637Alias Pifithrin-β, PFT-β, PFT β (hydrobromide), Cyclic Pifithrin-α hydrobromide, Cyclic PFT-αCas No. 511296-88-1

Pack Size	Price	USA Warehouse	Global Warehouse
2 mg	$34	In Stock	In Stock
5 mg	$51	In Stock	In Stock
10 mg	$85	In Stock	In Stock
25 mg	$153	In Stock	In Stock
50 mg	$223	In Stock	In Stock
100 mg	$332	In Stock	In Stock
1 mL x 10 mM (in DMSO)	$53	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Purity:99.74%

Color:White

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COA LCMS HNMR

Product Introduction

Bioactivity

Description	Pifithrin-β hydrobromide (Cyclic PFT-α) is an inhibitor of p53; reversibly blocks p53-dependent transcriptional activation and apoptosis. Protects against neuronal death in models of stroke and neurodegenerative disorders. Active in vivo; protects mice from the side-effects of Y therapy associated with p53 induction. Supresses self-renewal of embryonic stem cells. Also aryl hydrocarbon receptor (AHR) agonist, causes upregulation of AHR target gene CYP1A1 (EC50 = 1.1 μM).
Targets&IC50	p53:23 μM(EC50)
In vitro	PFTα molecule could not take a planar conformation required for AhR activation whereas Pifithrin-β hydrobromide showed a conformation similar to those of the prototypical AhR ligand β-naphthoflavone. In both cell lines, PFTα and Pifithrin-β hydrobromide provoked different responses related with AhR activation. However, when cyclization of PFTα to Pifithrin-β hydrobromide was hampered by acetylation of the exocyclic nitrogen, all these responses were not observed. These results lead to the conclusion that the activation of the AhR is probably caused by Pifithrin-β hydrobromide instead of PFTα.
Synonyms	Pifithrin-β, PFT-β, PFT β (hydrobromide), Cyclic Pifithrin-α hydrobromide, Cyclic PFT-α

Chemical Properties

Molecular Weight	349.29
Formula	C₁₆H₁₇BrN₂S
Cas No.	511296-88-1
Smiles	Br.Cc1ccc(cc1)-c1cn2c3CCCCc3sc2n1
Relative Density.	no data available

Storage & Solubility Information

Storage

In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 13.89 mg/mL (39.77 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 1 mg/mL (2.86 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.8630 mL	14.3148 mL	28.6295 mL	143.1475 mL
5 mM	0.5726 mL	2.8630 mL	5.7259 mL	28.6295 mL
10 mM	0.2863 mL	1.4315 mL	2.8630 mL	14.3148 mL
20 mM	0.1431 mL	0.7157 mL	1.4315 mL	7.1574 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc