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INT-777

Name: INT-777
Brand: TargetMol
SKU: T11662L
Price: 139 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T11662LCas No. 1199796-29-6

Alias S-EMCA

INT-777 (S-EMCA) is a potent TGR5 agonist with an EC50 of 0.82 μM. INT-777 (S-EMCA) inhibits NLRP5-ASC inflammasome-mediated neuroinflammation via the TGR3/cAMP/PKA signaling pathway after subarachnoid hemorrhage in rats.

INT-777

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Purity: 99.78%

Catalog No. T11662LAlias S-EMCACas No. 1199796-29-6

Pack Size	Price	USA Warehouse	Global Warehouse
1 mg	$139	In Stock	In Stock
5 mg	$347	In Stock	In Stock
10 mg	$496	In Stock	In Stock
25 mg	$795	In Stock	In Stock
50 mg	$1,110	-	In Stock
100 mg	$1,550	-	In Stock
1 mL x 10 mM (in DMSO)	$381	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

This molecule is a custom-made product. TargetMol has an excellent synthesis team with the experience and capability to provide you with cost-effective products.If you have any questions, please feel free to contact us. We are committed to serving you wholeheartedly.

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Batch Information

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Purity:99.78%

Appearance:Solid

Color:White to Yellow

Resource Download

COA LCMS HNMR

Product Introduction

Bioactivity

Description	INT-777 (S-EMCA) is a potent TGR5 agonist with an EC50 of 0.82 μM. INT-777 (S-EMCA) inhibits NLRP5-ASC inflammasome-mediated neuroinflammation via the TGR3/cAMP/PKA signaling pathway after subarachnoid hemorrhage in rats.
Targets&IC50	TGR5:0.82 μM (EC50), CHO cells:3.44 μM (EC50), COS-1 cells:> 100 μM (EC50), HEK293 cells:0.36 μM (EC50), NCI-H716 cells:0.82 μM (EC50)
In vitro	INT-777 (3 μM) increases ATP production in the human enteroendocrine cell line NCI-H716 in a cAMP-dependent manner.[2] INT-777 (10 μM) decreased Isc and increased TEER when added to the serosal side of serosal-stripped distal colon segments. The effect of INT-777 on basal secretion was reduced in neuronal-free and TTX-treated muco-submucosal preparations.[3]
In vivo	INT-777 (1 μM/min/kg; orally) is a potent choleretic that prevents activation and binding of carboxy-CoA, favoring its biliary-hepatic shunt pathway in HF-fed TGR5-Tg males, and shows absorption and strong choleretic effect in mice.[1] INT-777 (30 mg/kg/day; orally) increases energy expenditure and reduces hepatic steatosis and adiposity in high-fat-fed TGR5-Tg mice.[2]
Synonyms	S-EMCA

Chemical Properties

Molecular Weight	450.65
Formula	C₂₇H₄₆O₅
Cas No.	1199796-29-6
Smiles	[H][C@@]12CC[C@H]([C@H](C)C[C@H](C)C(O)=O)[C@@]1(C)[C@@H](O)C[C@@]1([H])[C@@]2([H])[C@H](O)[C@H](CC)[C@]2([H])C[C@H](O)CC[C@]12C
Relative Density.	1.128 g/cm3 (Predicted)

Storage & Solubility Information

Storage

store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

Ethanol: 50 mg/mL (110.95 mM), Sonication is recommended.

DMSO: 30 mg/mL (66.57 mM), Sonication is recommended.

Solution Preparation Table

DMSO/Ethanol

	1mg	5mg	10mg	50mg
1 mM	2.2190 mL	11.0951 mL	22.1902 mL	110.9508 mL
5 mM	0.4438 mL	2.2190 mL	4.4380 mL	22.1902 mL
10 mM	0.2219 mL	1.1095 mL	2.2190 mL	11.0951 mL
20 mM	0.1110 mL	0.5548 mL	1.1095 mL	5.5475 mL
50 mM	0.0444 mL	0.2219 mL	0.4438 mL	2.2190 mL

Ethanol

	1mg	5mg	10mg	50mg
100 mM	0.0222 mL	0.1110 mL	0.2219 mL	1.1095 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc