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INT-777

Name: INT-777
Brand: TargetMol
SKU: T11662L
Price: 139 USD
Availability: InStock
Rating: 5 (10 reviews)

🥰Excellent

Catalog No. T11662LCas No. 1199796-29-6

Alias S-EMCA

INT-777 (S-EMCA) is a potent TGR5 agonist with an EC50 of 0.82 μM. INT-777 (S-EMCA) inhibits NLRP5-ASC inflammasome-mediated neuroinflammation via the TGR3/cAMP/PKA signaling pathway after subarachnoid hemorrhage in rats.

INT-777

🥰Excellent

Purity: 99.89%

Catalog No. T11662LAlias S-EMCACas No. 1199796-29-6

Pack Size	Price	Availability
1 mg	$139	In Stock
2 mg	$198	In Stock
5 mg	$347	In Stock
10 mg	$497	In Stock
25 mg	$838	In Stock
50 mg	$1,160	In Stock
100 mg	$1,560	In Stock
1 mL x 10 mM (in DMSO)	$382	In Stock

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This molecule is a custom-made product. TargetMol has an excellent synthesis team with the experience and capability to provide you with cost-effective products. However, due to objective factors, there is a small probability that the synthesis may not be successful during the R&D process. We appreciate your understanding. If you have any questions, please feel free to contact us. We are committed to serving you wholeheartedly.

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Batch Information

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Purity:99.89%

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COA HNMR LCMS

Product Introduction

Bioactivity

Description	INT-777 (S-EMCA) is a potent TGR5 agonist with an EC50 of 0.82 μM. INT-777 (S-EMCA) inhibits NLRP5-ASC inflammasome-mediated neuroinflammation via the TGR3/cAMP/PKA signaling pathway after subarachnoid hemorrhage in rats.
Targets&IC50	COS-1 cells:> 100 μM (EC50), TGR5:0.82 μM (EC50), NCI-H716 cells:0.82 μM (EC50), HEK293 cells:0.36 μM (EC50), CHO cells:3.44 μM (EC50)
In vitro	INT-777 (3 μM) increases ATP production in the human enteroendocrine cell line NCI-H716 in a cAMP-dependent manner.[2] INT-777 (10 μM) decreased Isc and increased TEER when added to the serosal side of serosal-stripped distal colon segments. The effect of INT-777 on basal secretion was reduced in neuronal-free and TTX-treated muco-submucosal preparations.[3]
In vivo	INT-777 (1 μM/min/kg; orally) is a potent choleretic that prevents activation and binding of carboxy-CoA, favoring its biliary-hepatic shunt pathway in HF-fed TGR5-Tg males, and shows absorption and strong choleretic effect in mice.[1] INT-777 (30 mg/kg/day; orally) increases energy expenditure and reduces hepatic steatosis and adiposity in high-fat-fed TGR5-Tg mice.[2]
Synonyms	S-EMCA

Chemical Properties

Molecular Weight	450.65
Formula	C₂₇H₄₆O₅
Cas No.	1199796-29-6
Smiles	[H][C@@]12CC[C@H]([C@H](C)C[C@H](C)C(O)=O)[C@@]1(C)[C@@H](O)C[C@@]1([H])[C@@]2([H])[C@H](O)[C@H](CC)[C@]2([H])C[C@H](O)CC[C@]12C
Relative Density.	1.128 g/cm3 (Predicted)

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

Ethanol: 50 mg/mL (110.95 mM), Sonication is recommended.

DMSO: 30 mg/mL (66.57 mM), Sonication is recommended.

Solution Preparation Table

DMSO/Ethanol

	1mg	5mg	10mg	50mg
1 mM	2.2190 mL	11.0951 mL	22.1902 mL	110.9508 mL
5 mM	0.4438 mL	2.2190 mL	4.4380 mL	22.1902 mL
10 mM	0.2219 mL	1.1095 mL	2.2190 mL	11.0951 mL
20 mM	0.1110 mL	0.5548 mL	1.1095 mL	5.5475 mL
50 mM	0.0444 mL	0.2219 mL	0.4438 mL	2.2190 mL

Ethanol

	1mg	5mg	10mg	50mg
100 mM	0.0222 mL	0.1110 mL	0.2219 mL	1.1095 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:

For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . TargetMol | Animal experiments

A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent

DMSO+30% PEG300+5% Tween 80+60% Saline/PBS/ddH₂O. So your working solution concentration is 2 mg/mL。

Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSO TargetMol | reagent

(mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.

Preparation method for in vivo formula: Take 50 μL DMSO TargetMol | reagent

main solution, add 300 μLPEG300 TargetMol | reagent

mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLSaline/PBS/ddH₂O TargetMol | reagent

mix well and clarify

For Reference Only. Please develop an appropriate dissolution method based on your laboratory animals and route of administration.

All types of co-solvents required for the protocol, such asDMSO, PEG300/ PEG400, Tween 80, SBE-β-CD, corn oil are available for purchase on the TargetMol website with a simple click.

Dose Conversion

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