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GW6471

Name: GW6471
Brand: TargetMol
SKU: T8486
Price: 30 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T8486Cas No. 880635-03-0

Alias GW 6471

GW6471 is an antagonist of PPARα with IC50 of 0.24 μ M. GW6471 enhances the binding affinity of the PPAR α ligand-binding domain to the co-repressor proteins SMRT and NCoR.

GW6471

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Purity: 99.55%

Catalog No. T8486Alias GW 6471Cas No. 880635-03-0

GW6471 is an antagonist of PPARα with IC50 of 0.24 μ M. GW6471 enhances the binding affinity of the PPAR α ligand-binding domain to the co-repressor proteins SMRT and NCoR.

Pack Size	Price	USA Warehouse	Global Warehouse
2 mg	$30	In Stock	In Stock
5 mg	$48	In Stock	In Stock
10 mg	$81	In Stock	In Stock
25 mg	$186	In Stock	In Stock
50 mg	$328	In Stock	In Stock
100 mg	$562	In Stock	In Stock
200 mg	$845	-	In Stock
1 mL x 10 mM (in DMSO)	$89	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Batch Information

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Purity:99.55%

Appearance:Solid

Color:White to Yellow

Resource Download

COA HNMR LCMS

Product Introduction

Bioactivity

Description	GW6471 is an antagonist of PPARα with IC50 of 0.24 μ M. GW6471 enhances the binding affinity of the PPAR α ligand-binding domain to the co-repressor proteins SMRT and NCoR.
Targets&IC50	PPARγ (Cellular Binding):210 ± 64 nM, PPARα (Cellular Binding):254 ± 96 nM, PPARα (Molecular Level Binding):95.3 ± 42.0 nM, PPARγ (Molecular Level Binding):39.4 ± 18.2 nM
In vitro	METHODS: RCC cells Caki-1 (VHL wild type) and 786-O (VHL mutant) were treated with GW6471 (12.5-100 µM) for 72 h. Cell viability was measured using MTT assay.
In vivo	METHODS: To assay in vivo antitumor activity, GW6471 (20 mg/kg) was injected intraperitoneally every two days for four weeks into Nu/Nu mice bearing the RCC tumor Caki-1. RESULTS: In vivo treatment of xenograft mouse model with PPARα antagonist GW6471 attenuated RCC growth. [2]
Synonyms	GW 6471

Chemical Properties

Molecular Weight	619.67
Formula	C₃₅H₃₆F₃N₃O₄
Cas No.	880635-03-0
Smiles	CCC(=O)NC[C@H](Cc1ccc(OCCc2nc(oc2C)-c2ccccc2)cc1)N\C(C)=C/C(=O)c1ccc(cc1)C(F)(F)F
Relative Density.	1.204 g/cm3 (Predicted)

Storage & Solubility Information

Storage

In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 16.67 mg/mL (26.9 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+90% Saline: 0.25 mg/mL (0.4 mM), Solution.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	1.6138 mL	8.0688 mL	16.1376 mL	80.6881 mL
5 mM	0.3228 mL	1.6138 mL	3.2275 mL	16.1376 mL
10 mM	0.1614 mL	0.8069 mL	1.6138 mL	8.0688 mL
20 mM	0.0807 mL	0.4034 mL	0.8069 mL	4.0344 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc