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Entecavir monohydrate

Name: Entecavir monohydrate
Brand: TargetMol
SKU: T0085
Price: 30 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T0085Cas No. 209216-23-9

Alias SQ 34676, Entecavir hydrate, BMS-200475

Entecavir monohydrate (BMS-200475) is a selective inhibitor of the replication of the hepatitis B virus (HBV). It, a new deoxyguanine nucleoside analog.

Entecavir monohydrate

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Purity: 99.73%

Catalog No. T0085Alias SQ 34676, Entecavir hydrate, BMS-200475Cas No. 209216-23-9

Entecavir monohydrate (BMS-200475) is a selective inhibitor of the replication of the hepatitis B virus (HBV). It, a new deoxyguanine nucleoside analog.

Pack Size	Price	USA Warehouse	Global Warehouse
2 mg	$30	In Stock	In Stock
5 mg	$44	In Stock	In Stock
10 mg	$77	In Stock	In Stock
25 mg	$116	In Stock	In Stock
50 mg	$162	In Stock	In Stock
100 mg	$243	In Stock	In Stock
1 mL x 10 mM (in DMSO)	$48	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Purity:99.73%

Color:White

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COA HNMR HPLC

Product Introduction

Bioactivity

Description	Entecavir monohydrate (BMS-200475) is a selective inhibitor of the replication of the hepatitis B virus (HBV). It, a new deoxyguanine nucleoside analog.
Targets&IC50	HBV (HepG2 cells):3.75 nM(ec50)
In vitro	In ducks, Entecavir administration results in a 4-log reduction of serum DHBV DNA levels within 80 days and a 2- to 3-log decrease in hepatitis B virus surface antigen within 120 days. Treatment with Entecavir also decreases replication intermediates of DHBV DNA in the liver by a factor of 70, while covalently closed circular DNA levels are only reduced fourfold under stable template conditions. Additionally, Entecavir reduces both the intensity of antigen staining and the percentage of antigen-positive cells in the liver, although there is an increase in antigen staining intensity in bile duct cells.
In vivo	In liver biopsy samples, Entecavir reduces both covalently closed circular DNA and the negativity of the hepatitis B virus core antigen. Entecavir triphosphate acts as an effective inhibitor of the wild-type HBV polymerase and is 100-300 times more potent than lamivudine triphosphate against 3TC-resistant HBV polymerase. To inhibit the replication of 3TC-resistant HBV, Entecavir requires a concentration that is 20-30 times higher. With an EC50 of 0.1 nM, Entecavir demonstrates significant activity against HIV. It can be utilized in a single-cycle, single-cell line pseudovirus assay, measuring green fluorescent protein in CD4+ lymphocytes using fluorescence-activated cell sorting.
Cell Research	BMS 200475 is prepared in phosphate-buffered saline (PBS) and diluted with appropriate medium containing 2% fetal bovine serum. HepG2 2.2.15 cells are plated at a density of 5×105 cells per well on 12-well Biocoat collagen-coated plates and are maintained in a confluent state for 2 to 3 days before being overlaid with 1 mL of medium spiked with BMS 200475. Quantification of HBV was performed on day 10[1].
Synonyms	SQ 34676, Entecavir hydrate, BMS-200475

Chemical Properties

Molecular Weight	295.3
Formula	C₁₂H₁₅N₅O₃·H₂O
Cas No.	209216-23-9
Smiles	O.Nc1nc(=O)c2ncn([C@H]3C[C@H](O)[C@@H](CO)C3=C)c2[nH]1
Relative Density.	1.81

Storage & Solubility Information

Storage

keep away from direct sunlight,store under nitrogen | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 55 mg/mL (186.25 mM), Sonication is recommended.

Ethanol: < 1 mg/mL (insoluble or slightly soluble)

H2O: < 1 mg/mL (insoluble or slightly soluble)

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 2.5 mg/mL (8.47 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	3.3864 mL	16.9319 mL	33.8639 mL	169.3193 mL
5 mM	0.6773 mL	3.3864 mL	6.7728 mL	33.8639 mL
10 mM	0.3386 mL	1.6932 mL	3.3864 mL	16.9319 mL
20 mM	0.1693 mL	0.8466 mL	1.6932 mL	8.4660 mL
50 mM	0.0677 mL	0.3386 mL	0.6773 mL	3.3864 mL
100 mM	0.0339 mL	0.1693 mL	0.3386 mL	1.6932 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc