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DIF-3

Catalog No. T60485 CAS 113411-17-9

DIF-3 is a chemical compound that acts by activating GSK-3β to facilitate the degradation of cyclin D1 and c-Myc, resulting in reduced expression levels of these proteins. In addition, DIF-3 inhibits Wnt/β-catenin signaling pathway-related proteins in DLD-1 cells. This compound exerts a potent antiproliferative effect on the HeLa human cervical cancer cell line by inducing cyclin D1 degradation and inhibiting cyclin D1 mRNA expression [1].

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DIF-3, CAS 113411-17-9

Pack Size	Availability	Price/USD
1 mg	In stock	$ 93.00
5 mg	In stock	$ 247.00
10 mg	In stock	$ 369.00
25 mg	In stock	$ 619.00
50 mg	In stock	$ 883.00
100 mg	In stock	$ 1,190.00
500 mg	In stock	$ 2,430.00
1 mL * 10 mM (in DMSO)	In stock	$ 227.00

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Description

In vitro

In this study, we examined the effects of six DIF analogues (DIF-1, DIF-2 (which has pentanone in place of hexanone), DIF-3 (dechlorinated form of DIF-1), 2-MIDIF-1 (2-methoxy isomer of DIF-1), DMPH (dechlorinated form of DIF-3), and THPH (4-hydroxy substitution of DMPH)) on DNA synthesis, cell growth, erythroid differentiation, and cytosolic free calcium concentration ([Ca2+]i) in human leukemia K562 cells. DIF-3 proved to be the most potent anti-leukemic agent among them, and the order of potency for causing growth inhibition, erythroid induction, and increases in [Ca2+]iTHPH in all the categories tested. [1] DIF-3 activates GSK-3beta to accelerate the proteolysis of cyclin D1 and that this mechanism is involved in the DIF-3-induced G(0)/G(1) arrest in mammalian cells.[2]

Molecular Weight	272.72
Formula	C13H17ClO4
CAS No.	113411-17-9

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 90.0 mg/mL (330.0 mM), Sonication is recommended.

References and Literature

1. Kubohara Y. Effects of differentiation-inducing factors of Dictyostelium discoideum on human leukemia K562 cells: DIF-3 is the most potent anti-leukemic agent. Eur J Pharmacol. 1999;381(1):57-62. 2. Takahashi-Yanaga F, et al. Dictyostelium differentiation-inducing factor-3 activates glycogen synthase kinase-3beta and degrades cyclin D1 in mammalian cells. J Biol Chem. 2003;278(11):9663-9670.

Citations

1. Zheng C, Wang Y, Bi B, et al.Gallic acid ameliorates endometrial hyperplasia through the inhibition of the PI3K/AKT pathway and the down-regulation of cyclin D1 expression.Journal of Pharmacological Sciences.2024

Related compound libraries

This product is contained In the following compound libraries：

Anti-Cancer Active Compound Library Kinase Inhibitor Library Metabolism Compound Library Bioactive Compounds Library Max Bioactive Compound Library Anti-Cancer Compound Library Anti-Obesity Compound Library Cytoskeletal Signaling Pathway Compound Library

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Keywords

DIF-3 113411-17-9 inhibitor inhibit

Products are chemical reagents for research use only and are not intended for human use. We do not sell to patients.