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Anti-CCND1 Polyclonal Antibody is a Rabbit antibody targeting CCND1. Anti-CCND1 Polyclonal Antibody can be used in WB,IHC-P,ICC/IF,IHC-Fr,IF.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $222 | 7-10 days | |
100 μL | $372 | 7-10 days | |
200 μL | $529 | 7-10 days |
Description | Anti-CCND1 Polyclonal Antibody is a Rabbit antibody targeting CCND1. Anti-CCND1 Polyclonal Antibody can be used in WB,IHC-P,ICC/IF,IHC-Fr,IF. |
Ig Type | IgG |
Reactivity | Human,Mouse,Rat |
Verified Activity | 1. 25 μg total protein per Lane of various lysates probed with Cyclin D1 polyclonal antibody, unconjugated (TMAB-00317) at 1:2000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at RT for 60 min. 2. Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Cyclin D1 Polyclonal Antibody, Unconjugated (TMAB-00317) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Cyclin D1 Polyclonal Antibody, Unconjugated (TMAB-00317) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Cyclin D1 Polyclonal Antibody, Unconjugated (TMAB-00317) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 5. Paraformaldehyde-fixed, paraffin embedded Rat Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Cyclin D1 Polyclonal Antibody, Unconjugated (TMAB-00317) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 6. Paraformaldehyde-fixed, paraffin embedded Mouse Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Cyclin D1 Polyclonal Antibody, Unconjugated (TMAB-00317) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 7. Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Cyclin D1 Polyclonal Antibody, Unconjugated (TMAB-00317) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 8. Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Cyclin D1 Polyclonal Antibody, Unconjugated (TMAB-00317) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. |
Application | WB,IHC-P,ICC/IF,IHC-Fr,IF |
Recommended Dose | WB: 1:500-2000; IHC-P: 1:50-200; ICC/IF: 1:50-200; IHC-Fr: 1:50-200; IF: 1:50-200 |
Antibody Type | Polyclonal |
Host Species | Rabbit |
Subcellular Localization | Nucleus. Cytoplasm. Membrane. Note=CyclinD-CDK4 complexes accumulate at the nuclear membrane and are thentranslocated to the nucleus through interaction with KIP/CIP familymembers. |
Construction | Hybridoma Polyclonal Antibody |
Purification | Protein A purified |
Appearance | Liquid |
Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
Research Background | The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of tumors and may contribute to tumorigenesis. [provided by RefSeq, Jul 2008]. |
Immunogen | KLH conjugated synthetic peptide: human Cyclin D1 |
Antigen Species | Human |
Gene ID | |
Uniprot ID | |
Biology Area | Cyclin D family,Cyclin D Family,Cyclin D Family |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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