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Product Introduction

Bioactivity

Description	Brilliant blue G-250 (Acid blue 90, Coomassie Brilliant Blue G) is an antagonist of the P2X7 purinergic receptor.
In vitro	Brilliant Blue G-250 is used for protein staining after polyacrylamide gel electrophoresis Instructions 1. The protein electrophoresis gel is fixed in 50% methanol, 10% acetic acid, and 40% aqueous solution, and shaken at a low speed on a horizontal shaker for 30 min to overnight. 2. Remove the fixative and replace with 0.25% Coomassie Brilliant Blue R250 staining solution (0.25% R250 dissolved in 50% methanol, 10% acetic acid, and 40% aqueous solution) to completely cover the gel and stain for 2-4 h. Until the gel is stained uniformly blue. Note: When the gel is invisible to the naked eye in the staining solution, it means that the staining is complete, otherwise the gel area looks lighter in color compared to the dark staining solution. 3. Place the gel back in 5% methanol, 7.5% acetic acid, and 87.5% aqueous solution for 4-24 h for decolorization. The protein bands will be visible after about 1-2 hours, and the destaining process will not be completed until the background becomes transparent. The destaining solution can be changed 2-4 times in the middle. 4. Store the gel in 7% acetic acid. It can also be stored in water or dry.
Alias	Brilliant Blue G, BBG, Acid blue 90, Coomassie Brilliant Blue G

Chemical Properties

Molecular Weight	854.02
Formula	C₄₇H₄₈N₃NaO₇S₂
Cas No.	6104-58-1
Smiles	O=S(C1=CC=CC(CN(CC)C2=CC=C(/C(C3=CC=C(NC4=CC=C(OCC)C=C4)C=C3)=C5C(C)=C/C(C=C/5)=[N+](CC)\CC6=CC=CC(S(=O)([O-])=O)=C6)C(C)=C2)=C1)([O-])=O.[Na+]
Relative Density.	1.274 g/cm3. Temperature:20 °C.

Storage & Solubility Information

Storage

keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 8.54 mg/mL (10 mM), Sonication is recommended.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	1.1709 mL	5.8547 mL	11.7093 mL	58.5466 mL
5 mM	0.2342 mL	1.1709 mL	2.3419 mL	11.7093 mL
10 mM	0.1171 mL	0.5855 mL	1.1709 mL	5.8547 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:

For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . TargetMol | Animal experiments

A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent

DMSO+30% PEG300+5% Tween 80+60% Saline/PBS/ddH₂O. So your working solution concentration is 2 mg/mL。

Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSO TargetMol | reagent

(mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.

Preparation method for in vivo formula: Take 50 μL DMSO TargetMol | reagent

main solution, add 300 μLPEG300 TargetMol | reagent

mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLSaline/PBS/ddH₂O TargetMol | reagent

mix well and clarify

For Reference Only. Please develop an appropriate dissolution method based on your laboratory animals and route of administration.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Sci Citations

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc