Brilliant blue G-250 (Acid blue 90, Coomassie Brilliant Blue G) is an antagonist of the P2X7 purinergic receptor.

Brilliant Blue G-250 is used for protein staining after polyacrylamide gel electrophoresis
Instructions
1. The protein electrophoresis gel is fixed in 50% methanol, 10% acetic acid, and 40% aqueous solution, and shaken at a low speed on a horizontal shaker for 30 min to overnight.
2. Remove the fixative and replace with 0.25% Coomassie Brilliant Blue R250 staining solution (0.25% R250 dissolved in 50% methanol, 10% acetic acid, and 40% aqueous solution) to completely cover the gel and stain for 2-4 h. Until the gel is stained uniformly blue.
Note: When the gel is invisible to the naked eye in the staining solution, it means that the staining is complete, otherwise the gel area looks lighter in color compared to the dark staining solution.
3. Place the gel back in 5% methanol, 7.5% acetic acid, and 87.5% aqueous solution for 4-24 h for decolorization. The protein bands will be visible after about 1-2 hours, and the destaining process will not be completed until the background becomes transparent. The destaining solution can be changed 2-4 times in the middle.
4. Store the gel in 7% acetic acid. It can also be stored in water or dry.