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Azithromycin

Name: Azithromycin
Brand: TargetMol
SKU: T6401
Price: 30 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T6401Cas No. 83905-01-5

Alias Zithromax, XZ-450, CP 62993

Azithromycin (CP 62993) is a macrolide antibiotic that primarily targets the 50S subunit of bacterial ribosomes. Azithromycin is commonly used in studies of bacterial infections.

Azithromycin

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Purity: 99.97%

Catalog No. T6401Alias Zithromax, XZ-450, CP 62993Cas No. 83905-01-5

Azithromycin (CP 62993) is a macrolide antibiotic that primarily targets the 50S subunit of bacterial ribosomes. Azithromycin is commonly used in studies of bacterial infections.

Pack Size	Price	USA Warehouse	Global Warehouse
10 mg	$30	In Stock	In Stock
25 mg	$41	In Stock	In Stock
50 mg	$56	In Stock	In Stock
100 mg	$84	In Stock	In Stock
500 mg	$219	In Stock	In Stock
1 mL x 10 mM (in DMSO)	$62	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Purity:99.97%

Color:White

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COA LCMS HNMR

Product Introduction

Bioactivity

Description	Azithromycin (CP 62993) is a macrolide antibiotic that primarily targets the 50S subunit of bacterial ribosomes. Azithromycin is commonly used in studies of bacterial infections.
Targets&IC50	NF-κB:56 μM (A549 cells), IL-1β:17.4 μM, Parasite growth:8.4 ± 1.2 μM, PBMC cells proliferation:5 µg/mL, TNF-α:10.66 μM
In vitro	METHODS: HeLa, MG-63 and Osteoblast cells were treated with Azithromycin (0-400 μg/mL) for 48 hours, and MTT assay was used to detect the inhibition of cell metabolic activity. RESULTS: Azithromycin inhibited the growth of HeLa (IC50=160 μM), MG-63 (IC50=180 μM) and Osteoblast (IC50=160 μM) cells. [1]
In vivo	METHODS: To study the effect of Azithromycin on asthma, a mouse model of asthma attack was treated with Azithromycin (50 mg/kg). RESULTS: Azithromycin had no effect on bronchoalveolar lavage inflammatory parameters and LDH levels in a mouse model of asthma exacerbation. Azithromycin did not induce general inflammatory parameters or LDH release in a mouse model of asthma exacerbations and increased expression of an interferon-stimulated gene and the pattern recognition receptor MDA5 but not RIG-I in mice with asthma exacerbations. [2]
Cell Research	THP-1 cells (106 cells in 1 mL RPMI medium, without antibiotics, growth factors or serum) are seeded in each well of 24-well plates and allowed to settle for 1 hour. Next, 50 μL of the test compound is added followed by 50 μL of LPS (final concentration of 10 μg/mL). After 24h (37°C and 5% CO2) the supernatants and cell pellets are collected (1200 rpm, 5 min). THP-1 cell viability is tested using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT). MTT is dissolved at 2 mg/mL in PBS and aliquots are stored at -20°C. The MTT assay is performed according to the suppliers instructions. Absorbance of MTT converted into formazan is measured at a wavelength of 570 nm with background subtraction at 630 nm.
Synonyms	Zithromax, XZ-450, CP 62993

Chemical Properties

Molecular Weight	748.98
Formula	C₃₈H₇₂N₂O₁₂
Cas No.	83905-01-5
Smiles	O([C@@H]1[C@@H](C)[C@H](O[C@H]2C[C@](OC)(C)[C@@H](O)[C@H](C)O2)[C@@H](C)C(=O)O[C@H](CC)[C@@](C)(O)[C@H](O)[C@@H](C)N(C)C[C@H](C)C[C@@]1(C)O)[C@H]3[C@H](O)[C@@H](N(C)C)C[C@@H](C)O3
Relative Density.	1.18 g/cm3 (Predicted)

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 18.33 mg/mL (24.47 mM), Sonication is recommended.

Ethanol: 74.9 mg/mL (100 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 2 mg/mL (2.67 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO/Ethanol

	1mg	5mg	10mg	50mg
1 mM	1.3351 mL	6.6757 mL	13.3515 mL	66.7575 mL
5 mM	0.2670 mL	1.3351 mL	2.6703 mL	13.3515 mL
10 mM	0.1335 mL	0.6676 mL	1.3351 mL	6.6757 mL
20 mM	0.0668 mL	0.3338 mL	0.6676 mL	3.3379 mL

Ethanol

	1mg	5mg	10mg	50mg
50 mM	0.0267 mL	0.1335 mL	0.2670 mL	1.3351 mL
100 mM	0.0134 mL	0.0668 mL	0.1335 mL	0.6676 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc