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AZ-5104

Name: AZ-5104
Brand: TargetMol
SKU: T2491
Price: 48 USD
Availability: InStock
Rating: 5 (10 reviews)

🥰Excellent

Catalog No. T2491Cas No. 1421373-98-9

AZ5104 is a potent EGFR inhibitor.

AZ-5104

🥰Excellent

Purity: 99.59%

Catalog No. T2491Cas No. 1421373-98-9

AZ5104 is a potent EGFR inhibitor.

Pack Size	Price	USA Warehouse	Global Warehouse
5 mg	$48	In Stock	In Stock
10 mg	$68	In Stock	In Stock
25 mg	$125	In Stock	In Stock
50 mg	$197	In Stock	In Stock
100 mg	$265	In Stock	In Stock
200 mg	$333	In Stock	In Stock
1 mL x 10 mM (in DMSO)	$53	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Batch Information

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Purity:99.59%

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COA

Product Introduction

Bioactivity

Description	AZ5104 is a potent EGFR inhibitor.
Targets&IC50	EGFR (L858R):6 nM, ERB4:7 nM, EGFR (WT):25 nM, EGFR (L861Q):1 nM, EGFR (L858R/T790M):<1 nM
In vitro	AZ5104 shows great potency against ex19del (2 nM in PC-9), T790M (2 nM in H1975), and wild-type EGFR (33 nM in LOVO) cell lines. AZ5104 causes inhibition of cell viability with IC50 of 3.3 nM, 2.6 nM, 80 nM, and 53 nM for H1975 (T790M/L858R), PC-9 (ex19del), Calu 3 (WT), and NCI-H2073 (WT), respectively. [1]
In vivo	In both C/L858R and C/L+T mice, AZ5104 (5 mg/kg/d, p.o.) induces significant and sustained tumor regression. [1]
Kinase Assay	Recombinant Kinase assays: Kinase assays are performed using peptide or protein substrates in a filter-binding radioactive ATP transferase assay for protein kinases, or lipid substrates and HTRF assay for lipid kinase.

Chemical Properties

Molecular Weight	485.58
Formula	C₂₇H₃₁N₇O₂
Cas No.	1421373-98-9
Smiles	COc1cc(N(C)CCN(C)C)c(NC(=O)C=C)cc1Nc1nccc(n1)-c1c[nH]c2ccccc12
Relative Density.	1.267 g/cm3 (Predicted)

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

Ethanol: 16 mg/mL (32.95 mM), Sonication is recommended.

H2O: < 1 mg/mL (insoluble or slightly soluble)

DMSO: 90 mg/mL (185.35 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 3.3 mg/mL (6.8 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

Ethanol/DMSO

	1mg	5mg	10mg	50mg
1 mM	2.0594 mL	10.2970 mL	20.5939 mL	102.9696 mL
5 mM	0.4119 mL	2.0594 mL	4.1188 mL	20.5939 mL
10 mM	0.2059 mL	1.0297 mL	2.0594 mL	10.2970 mL
20 mM	0.1030 mL	0.5148 mL	1.0297 mL	5.1485 mL

DMSO

	1mg	5mg	10mg	50mg
50 mM	0.0412 mL	0.2059 mL	0.4119 mL	2.0594 mL
100 mM	0.0206 mL	0.1030 mL	0.2059 mL	1.0297 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:

For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . TargetMol | Animal experiments

A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent

DMSO+30% PEG300+5% Tween 80+60% Saline/PBS/ddH₂O. So your working solution concentration is 2 mg/mL。

Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSO TargetMol | reagent

(mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.

Preparation method for in vivo formula: Take 50 μL DMSO TargetMol | reagent

main solution, add 300 μLPEG300 TargetMol | reagent

mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLSaline/PBS/ddH₂O TargetMol | reagent

mix well and clarify

For Reference Only. Please develop an appropriate dissolution method based on your laboratory animals and route of administration.

All types of co-solvents required for the protocol, such asDMSO, PEG300/ PEG400, Tween 80, SBE-β-CD, corn oil are available for purchase on the TargetMol website with a simple click.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc